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Role of phytoestrogenic oils in alleviating osteoporosis associated with ovariectomy in rats

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Abstract

The objective of this study was to elucidate the effect of soybean oil (SbO) and sesame oil (SO) supplemented diets on bone biomarkers changes in OVX (ovariectomized) rats. The current data exhibited significant decrease in BMD (bone mineral density), accompanied with marked depletion in the level of Ca, P and Mg in both serum and bone of OVX rats. Also, serum estrogen, total protein, HDL-C (high density lipoprotein cholesterol), bone NO levels were decreased in OVX rats. However, a significant increase in the level of serum TL (total lipids), TC (total cholesterol), TG (triglycerides), LDL-C (low density lipoprotein cholesterol), VLDL-C (very low density lipoprotein cholesterol), urine minerals (Ca, P, Mg), as well as serum, bone and urine ALP (alkaline phosphatase) and ACP (acid phosphatase) activity were recorded in OVX rats. Further changes were also detected by the increased level of urine hydroxyproline, serum parathyroid hormone and osteocalcin, as well as urea and creatinine level in both serum and urine. On the other hand, when OVX rats were fed on SbO (soy bean oil) (15 % w/w) or SO (sesame oil) (10 % w/w) supplemented diets, the data recorded a significant improvement in all the above mentioned parameters. So, it can be concluded that consumption of SbO or SO supplemented diets might be considered as a functional food for retarding risks of osteoporosis associated with estrogen deficiency in OVX states.

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Abbreviations

NC:

Normal control

SbO:

Soy bean oil

SO:

Sesame oil

OVX:

Ovariectomized

BMD:

Bone mineral density

TL:

Total lipids

TC:

Total cholesterol

TG:

Triglycerides

HDL-C:

High density lipoprotein cholesterol

LDL-C:

Low density lipoprotein cholesterol

VLDL-C:

Very low density lipoprotein cholesterol

ALP:

Alkaline phosphatase

ACP:

Acid phosphatase

NO:

Nitric oxide

Ca:

Calcium

P:

Phosphorus

Mg:

Magnesium

References

  • Adlercreutz H, Goldin BR, Gorbach SL, Hockerstedt KAV, Watanabe S, Hamalainen EK, Markkanen MH, Makela TH, Wahala KT, Hase TA, Fotsis T (1995) Soybean phytoestrogen intake and cancer risk. J Nutr 125:757–770

    Google Scholar 

  • Ahmedi SA, BorouMand MA, Moghddam KG, Tajik P, Dibaj SM (2008) The impact of low serum triglyceride on LDL-cholesterol estimation. Arch Iran Med 11:318–321

    Google Scholar 

  • An YH, Freidman RJ (1998) Animal models in orthopaedic. Research. Boca Raton: CRC press and bone health: a brief overview. J Ren Nutr 10:63–68

    Google Scholar 

  • Anwar A, McTernan PG, Anderson LA, Askaa J, Moody CG, Barnett AH (2001) Site-specific regulation of oestrogen receptor-alpha and—beta by oestradiol in human adipose tissue. Diabetes Obes Metab 5:338–349

    Article  Google Scholar 

  • Arjmandi BH, Birnbaum R, Goyal NV, Getlinger MJ, Juma S, Alekel L, Hasler CM, Drum ML, Hollis BW, Kukreja SC (1998) Bone-sparing effect of soy protein in ovarian hormone-deficient rats is related to its isoflavone content. J Am Clin Nutr 68:1364–1368

    Google Scholar 

  • Barnes S (2004) Supplement: fifth internat’l symposium on the role of soy in preventing and treating chronic disease soy isoflavones-phytoestrogens and what else? J Nutr 134:1225–1228

    Google Scholar 

  • Binbin LI, Shifeng YU (2003) Genistein prevents bone resorption diseases by inhibiting bone resorption and stimulating bone formation. Biol Pharm Bull 26:780–786

    Article  Google Scholar 

  • Boulbaroud S, Mesfioui A, Arfaoui A, Ouichou A, El- Hessni A (2008) Preventive effects of flaxseed and sesame oil on bone loss in ovariectomized rats. J Pak Biol Sci 11:1696–1701

    Article  CAS  Google Scholar 

  • Briante R, La Cara F, Tonziello MP, Febbraio F, Nucci R (2001) Antioxidant activity of the main bioactive derivatives from oleuropein hydrolysis by hyperthermophilic beta-glycosidase. J Agric Food Chem 49:3198–3203

    Article  CAS  Google Scholar 

  • Brouns F (2002) Soya isoflavones: a new and promising ingredient for the health food sector. Food Res Int 35:187–193

    Article  CAS  Google Scholar 

  • Chen J, Hui E, Ip T, Thompson LU (2004) Dietary flaxseed enhances the inhibitory effect of tamoxifen on the growth of estrogen dependent human breast cancer (MCF-7) in nude mice. Clin Cancer Res 10:7703–7711

    Article  CAS  Google Scholar 

  • Chen Z, Bassford T, Green SB, Cauley GA, Jackson RD, La Croix AZ (2005) Postmenopausal hormone therapy and body composition – a substudy of the estrogen plus progestin trial of the womens’s health initiative. J Am Clin Nutr 82:651–656

    CAS  Google Scholar 

  • Chen J, Singhal R, Lazarenko OP, Badger TM, Ronis MJ (2008) Promotion of bone growth by dietary soy protein isolate: comparision with dietary casein, whey hydrolysate and rice protein isolate in growing female rats. J FASEB 22:303–305

    Google Scholar 

  • Chiang JY (2003) Bile acid regulation of hepatic physiology III. Bile acids and nuclear receptors. J Am Physiol Gastrointest Liver Physiol 284:349–356

    Google Scholar 

  • Chiechi LM, Micheli L (2005) Utility of dietary phytoestrogens in preventing postmenopausal osteoporosis. Nutraceutic Res 3:15–28

    CAS  Google Scholar 

  • Chiechi LM, Secreto G, Vimercati A, Greco P, Venturelli E, Pansini F, Fanelli M, Loizzi P, Selvaggi L (2002) The effects of a soy rich diet on serum lipids, the Menfis randomized trial. J Matur 41:97–104

    Article  CAS  Google Scholar 

  • Chilibeck PD, Cornish SM (2008) Effect of estrogenic compounds (estrogen or phytorsteogens) combined with exercise on bone and muscle mass in order individuals. Appl Physiol Nutr Metab 33:200–212

    Article  CAS  Google Scholar 

  • Cho SJ, Juillerat MA, Lee CH (2008) Identification of LDL-receptor transcription stimulating peptides from soybean hydrolysate in human hepatocytes. J Agric Food Chem 56:4372–4376

    Article  CAS  Google Scholar 

  • Choi JS, Song J (2009) Effect of genistein on insulin resistance, renal lipid metabolism, and antioxidative activities in ovariectomized rats. J Nutr 25:676–685

    Article  CAS  Google Scholar 

  • Cornwell T, Cohick W, Raskin I (2004) Dietary phytoestrogens and health. Phytoche 65:995–1016

    Article  CAS  Google Scholar 

  • Cynthia KS, Brian CC, Michael JT, Amalia G, Ali A, Stephen B (2008) Effect of a daily supplement of soy protein on body composition and insulin secretion in postmenopausal women. J Rev 88:1609–1617

    Google Scholar 

  • Deshaies Y, Dagnault A, Lalonde J, Richard D (1997) Interaction of corticosterone and gonadal steroids on lipid deposition in the female rat. J Am Physiol 273:355–362

    Google Scholar 

  • Deyhima F, Stoeckerb BJ, Brusewitzc GH, Arjmandib BH (2003) The effects of estrogen depletion and isoflavones on bone metabolism in rats. Nutr Res 23:123–130

    Article  Google Scholar 

  • El-Wakf AM, Ibrahim HA, El-Sawy MR, El-Said FG (2003) Aging-related bone changes in rats: role of antioxidants. Egypt J Zool 40:506

    Google Scholar 

  • Fernandez ML (1995) Distinct mechanisms of plasma LDL lowering by dietary soluble fiber: specific effects of pectin, guar gum and psyllium. J Lipid Res 36:2394–2404

    CAS  Google Scholar 

  • Fernandez N, Renedo M, Garcia-Rodriguez C, Sanchez CM (2002) Activation of monocytic cells through Fc receptors induces the expression of macrophage-inflammatory protein (MIP)-1alpha, MIP-beta, and RANTES. J Immunol 169:3321–3328

    CAS  Google Scholar 

  • Gennari L, Becherini L, Falchetti A, Masi L, Massart F, Brandi ML (2002) Genetics of osteoporosis: role of steroid hormone receptor gene polymorphisms. J Steroid Biochem Biol 81:1–24

    Article  CAS  Google Scholar 

  • Guillemant J, Taupin P, Le H, Taright N, Allemandou A, Pérès G, Guillemant S (1999) Vitamin D status during puberty in French healthy male adolescents. Osteoporos Int 10:222–225

    Article  CAS  Google Scholar 

  • Hassan HA, Abdel-Wahhab MA (2012) Effect of soybean oil on atherogenic metabolic risks associated with estrogen deficiency in ovariectomized rats. J Physiol Biochem 68:247–253

    Article  CAS  Google Scholar 

  • He C, Hui R, Tezuka Y, Kadota S, Li J (2010) Osteoprotective effect of extract from achyranthes bidentata in ovariectomized rats. J Ethnopharmacol 127:229–234

    Article  Google Scholar 

  • Ima-Nirwana S, Ahmad NS, Yee LJ, Loh HC, Yew SF, Norazlina M, Abdul GMT, Kamsiah JS (2007) Reheating of soy oil is detrimental to bone metabolism in oestrogen deficient rats. J Med 48:200–206

    CAS  Google Scholar 

  • Ishimi Y, Miyaura C, Jin CH, Akatsu T, Abe E, Nakamura Y, Yamaguchi A, Yoshiki S, Matsuda T, Hirano T, Kishimoto T, Suda T (1990) IL-6 is produced by osteoblasts and induces bone resorption. J Immunol 145:3297–3303

    CAS  Google Scholar 

  • Johnson-Lynn SE, Francis R, McCaskie A (2008) Osteoporosis treatments and their effect on fracture healing. Curr Orthopaed 22:336–340

    Article  Google Scholar 

  • Kalu DN (1991) The ovariectomized rat model of postmenopausal bone loss. Bone Miner 15:175–191

    Article  CAS  Google Scholar 

  • Kasugai Y, Ikegami A, Matsuo K, Ohashi M, Sukamoto T, Hosoi T, Ouchi Y, Orimo H (1998) Effects of tibolone (Org OD14) treatment for 3 months on ovariectomy-induced osteopenia in 8-month-old rats on a low-calcium diet: preventive testing for 3 months. Bone 22:119–124

    Article  CAS  Google Scholar 

  • Keller ET, Binkley NC, Stebler BA, Hall DM, Johnston GM, Zhang J, Ershler WB (2000) Ovariectomy does not induce osteopenia through interleukin-6 in rhesus monkeys (Macaca mulatta). Bone 26:55–62

    Article  CAS  Google Scholar 

  • Knight DC, Eden JA (1996) A review of the clinical effects of phytoestrogens. Obst Gynecol 87:897–904

    CAS  Google Scholar 

  • Krivosíková Z, Krajcovicová-Kudlácková M, Spustová V, Stefíková K, Valachovicová M, Blazícek P, Nĕmcová T (2010) The association between high plasma homocysteine levels and lower bone mineral density in Slovak women: the impact of vegetarian diet. J Eur Nutr 49:147–153

    Article  Google Scholar 

  • Kuiper GG, Lemmen JG, Carlsson B, Corton JC, Safe SH, Van der Saag PT, Van der Burg B, Gustafsson JA (1998) Interaction of estrogenic chemicals and phytoestrogens with estrogen receptor β. J Endocrinol 139:4252–4263

    Article  CAS  Google Scholar 

  • Lau EM, Cooper C (1996) The epidemiology of osteoporosis: the oriental perspective in a world context. Clin Orthop Relat Res 323:65–74

    Article  Google Scholar 

  • Lerner UH (2006) Bone remodeling in post-menopausal osteoporosis. Dent J Res 85:584–595

    Article  CAS  Google Scholar 

  • Li S, He H, Ding M, He C (2010) The correlation of osteoporosis to clinical features: a study of 4382 female cases of a hospital cohort with musculoskeletal symptoms in southwest China BMC. Musculoskeletal 11:1471–2474

    Google Scholar 

  • Lien T, Hsu Y, Lo D, Chiou RY (2009) Supplementary health benefits of soy aglycons of isoflavone by improvement of serum biochemical attributes, enhancement of liver antioxidative capacities and protection of vaginal epithelium of ovariectomized rats. Nutr Metabol 6:6–15

    Article  Google Scholar 

  • Lund CO, Mortensen A, Nilas L, Breinholt VM, Larsen J (2007) Bent Ottesen estrogen and phytoestrogens: effect on eNOS expression and in vitro vasodilation in cerebral arteries in ovariectomized Watanabe heritable hyperlipidemic rabbits. Eur J Obst Gynecol Reprod Biol 130:84–92

    Article  CAS  Google Scholar 

  • Mancini L, Moradi-Bidhendi N, Brandi M, MacIntyre I (1998) Nitric oxide, superoxide and peroxynitrite modulate osteoclast activity. Biochem Biophys Res Commun 243:785–790

    Article  CAS  Google Scholar 

  • Matsumura Y, Kita S, Morimoto S, Akimoto K, Furuya M, Oka N, Tanaka T (1995) Antihypertensive effect of sesamin: protection against deoxycorticosterone acetate-salt-induced hypertension and cardiovascular hypertrophy. Biol Pharm Bull 18:1016–1019

    Article  CAS  Google Scholar 

  • Messina MJ, Persky V, Setchell KDR, Barnes S (1994) Soy intake and cancer risk: a review of the in vitro and in vivo data. J Nutr Can 21:113–131

    Article  CAS  Google Scholar 

  • Namkung-Matthai H, Appleyard R, Jansen J, Hao LJ, Maastricht S, Swain M, Mason RS, Murrell GA, Diwan AD, Diamond T (2001) Osteoporosis influences the early period of fracture healing in a rat osteoporotic model. Bone 28:80–86

    Article  CAS  Google Scholar 

  • Norazlina M, Ima-Nirwana S, Gapor MT, Khalid BA (2000) Palm vitamin E is comparable to alpha-tocopherol in maintaining bone mineral density in ovariectomised female rats. Exp Clin Endocrinol Diabetes 108:305–310

    Article  CAS  Google Scholar 

  • Ososki A, Kennelly EJ (2003) Phytoestrogens, a review of the present state of research. Phytother Res 17:845–869

    Article  CAS  Google Scholar 

  • Potter JD, Steinmetz K (1996) Vegetables, fruit and phytoestrogens as preventive agents. IARC Sci Publ 139:61–90

    CAS  Google Scholar 

  • Reinwald S, Weaver CM (2006) Soy isoflavones and bone health: a double- edged sword? J Nat Prod 69:450–459

    Article  CAS  Google Scholar 

  • Rodwell EW (1979) A review of physiological chemistery. In: Harper HA, Rodwell EW, Mayes P (eds), 17th edn. Lange Medical Publications, California, pp 401–404

  • Sacco SM, Power KA, Chen J, Ward WE, Thompson LU (2007) Interaction of sesame seed and tamoxifen on tumor growth and bone health in athymic mice. Exp Biol Med 232:754–761

    CAS  Google Scholar 

  • Sakai T, Kogiso M (2008) Soy isoflavones and immunity. Med J Invest 55:167–173

    Article  Google Scholar 

  • Sasaki Y, Ono H, Seki J, Giddings JC, Yamamoto J (2002) Effect of estrogen on the microcirculation and thrombus formation un pial vessels of the rat. Int Cong 1235:413–418

    Article  CAS  Google Scholar 

  • Satheesh M, Pari L (2008) Effect of pterostilbene on lipids and lipid profiles in streptozotocin–nicotinamide induced type 2 diabetes mellitus. J Appl Biomed 6:31–37

    CAS  Google Scholar 

  • Setchell KD (2001) Soy isoflavones-benefits and risks from nature’s selective estrogen receptor modulators (SERMs). J Am Coll Nutr 20:354–362

    Article  Google Scholar 

  • Shuid AN, Chuan LH, Mohamed N, Jaarin K, Fong YS, Soelaiman IN (2007) Recycled palm oil is better than soy oil in maintaining bone properties in a menopausal syndrome model of ovariectomized rat. J Asia Pac Clin Nutr 16:393–402

    CAS  Google Scholar 

  • Sok D, Cui HS, Kim MR (2009) Isolation and boactivities of furfuran type lignan compounds from edible plants. Nutr Agric 1:87–95

    CAS  Google Scholar 

  • Sowmya M, Jeyarani T, Indrani D (2009) Effect of replacement of fat with sesame oil and additives on rheological, microstructural, quality characteristics and fatty acid profile of cakes. Food Hydrocoll 23:1827–1836

    Article  CAS  Google Scholar 

  • Torres N, Torre-Villalvazo I, Tovar AR (2006) Regulation of lipid metabolism by soy protein and its implication in diseases mediated by lipid disorders. J Nutr Biochem 17:365–373

    Article  CAS  Google Scholar 

  • Van’t Hof RJ, Ralston SH (2007) Genetics of osteoporosis. J Nutr Soc 66:158–165

    Article  Google Scholar 

  • Van’t Hof RJ, Macphee J, Libouban H, Helfrich MH, Ralston SH (2004) Regulation of bone mass and bone turnover by neuronal nitric oxide synthase. Endocrinology 145:5068–5074

    Article  Google Scholar 

  • Veille JC, Li P, Eisenach JC (1996) Effect of estrogen on nitric oxide biosynthesis and vasorelaxant activity in sheep uterine arteries and renal arteries in vitro. J Am Obstet Gynecol 174:1043–1049

    Article  CAS  Google Scholar 

  • Waller RA, Duncan DB (1969) A Bayes rule for the symmetric multiple comparison problems. J Am Stat Assoc 64:1484–1503

    Google Scholar 

  • Wimalawansa SJ (2008) Nitric oxide: novel therapy for osteoporosis. Exp Pharmacol 9:3025–3044

    CAS  Google Scholar 

  • Wu W (2007) The contents of lignans in commercial sesame oils of Taiwan and their changes during heating. Food Chem 104:341–344

    Article  CAS  Google Scholar 

  • Wu T, Zhang Y, Yuan H, Wu G, Li B, Cui L (2008) Preventive effects of Salvianolic acid B on osteopenia of rats induced by ovariectomy. Bone 43:75–76

    Article  Google Scholar 

  • Ya S, Saga I, Ichimura I, Nagai T, Shinoda M, Matsuzki S (2003) Coumesterol as well as isoflavones in soybean extract prevent bone resorption in ovariectomized rats. Endocrin Regul 37:145–152

    Google Scholar 

  • Yamaguchi M, Sugimoto E (2000) Stimulatory effect of genistein and daidzein on protein synthesis in osteoblastic MC3T3-E1 cells, activation of aminoacyl-t-RNA synthetase. Mol Cell Biochem 214:97–102

    Article  CAS  Google Scholar 

  • Yamashita K, Iizuka Y, Imai T, Namiki M (1995) Sesame seed and its lignans produce marked enhancement of vitamin E activity in rats fed a low alpha-tocopherol diet. J Lipids 30:1019–10028

    Article  CAS  Google Scholar 

  • You L, Sheng Z, Tang C, Chen L, Pan L, Chen J (2011) High cholesterol diet increases osteoporosis risk via inhibiting bone formation in rats. Acta Pharmacol Sinica 32:1498–1504

    Article  CAS  Google Scholar 

  • Younes H, Garleb KA, Behr SR, Demigné C, Rémésy C (1998) Dietary fiber stimulates the extra-renal route of nitrogen excretion in partially nephrectomized rats. J Nutr Biochem 9:613–620

    Article  CAS  Google Scholar 

  • Zhang Y, Dong X, Leung P, Wong M (2009) Differential mRNA expression profiles in proximal tibia of aged rats in response to ovariectomy and low-Ca diet. J Bone 44:46–52

    Article  CAS  Google Scholar 

  • Zung A, Chalew SA (1997) Effect of age on the response to parathyroid hormone. Metabol 46:1246–1251

    Article  CAS  Google Scholar 

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  1. Physiology Division, Zoology Department, Faculty of Science, Mansoura University, Mansoura, Egypt

    Hanaa A. Hassan, Azza M. EL Wakf & Nermin E. EL Gharib

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  1. Hanaa A. Hassan
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  2. Azza M. EL Wakf
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  3. Nermin E. EL Gharib
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Correspondence to Hanaa A. Hassan.

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Hassan, H.A., EL Wakf, A.M. & EL Gharib, N.E. Role of phytoestrogenic oils in alleviating osteoporosis associated with ovariectomy in rats. Cytotechnology 65, 609–619 (2013). https://doi.org/10.1007/s10616-012-9514-6

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