Brief Report

Cytotechnology

, Volume 64, Issue 1, pp 1-7

Isolation and differentiation potential of an equine amnion-derived stromal cell line

  • Stefania VioliniAffiliated withParco Tecnologico Padano, CERSA
  • , Chiara GorniAffiliated withParco Tecnologico Padano, CERSA
  • , Laura Francesca PisaniAffiliated withParco Tecnologico Padano, CERSADepartment of Veterinary Pathology, Hygiene and Public Health, Faculty of Veterinary Medicine, Università degli Studi di Milano
  • , Paola RamelliAffiliated withParco Tecnologico Padano, CERSA
  • , Mario CaniattiAffiliated withDIPAV, Veterinary Anatomo-Pathology and Avian Pathology Unit, Università degli Studi di Milano
  • , Paola MarianiAffiliated withParco Tecnologico Padano, CERSA Email author 

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Abstract

Stem cells represent an important tool in veterinary therapeutic field such as tissue engineering. In the present study, equine amnion-derived mesenchymal stromal cells were investigated for applications in veterinary science as an alternative source to bone marrow mesenchymal stem cells and adipose stem cells. Amnion stromal cells isolation and characterization protocol is described; the in vitro cell growth rate was calculated by measuring viable cell number over 20 days. The expression of stem cell markers such as Oct-4, Nanog, Sox-2 and CD105 was assessed by retrotranscription quantitative PCR (RT-qPCR) and differentiation into adipocytes, osteocytes and chondrocytes precursors was analyzed by cytochemical staining. This study showed that amnion stromal cells expressing stem cell markers can differentiate into mesoderm lineage and may be an alternative source to mesenchymal stem cells derived from adipose tissue and bone marrow for the use in tissue repair.

Keywords

Equine Amnion Gene expression Differentiation