Cytotechnology

, Volume 64, Issue 1, pp 1–7

Isolation and differentiation potential of an equine amnion-derived stromal cell line

Authors

  • Stefania Violini
    • Parco Tecnologico Padano, CERSA
  • Chiara Gorni
    • Parco Tecnologico Padano, CERSA
  • Laura Francesca Pisani
    • Parco Tecnologico Padano, CERSA
    • Department of Veterinary Pathology, Hygiene and Public Health, Faculty of Veterinary MedicineUniversità degli Studi di Milano
  • Paola Ramelli
    • Parco Tecnologico Padano, CERSA
  • Mario Caniatti
    • DIPAV, Veterinary Anatomo-Pathology and Avian Pathology UnitUniversità degli Studi di Milano
    • Parco Tecnologico Padano, CERSA
Brief Report

DOI: 10.1007/s10616-011-9398-x

Cite this article as:
Violini, S., Gorni, C., Pisani, L.F. et al. Cytotechnology (2012) 64: 1. doi:10.1007/s10616-011-9398-x

Abstract

Stem cells represent an important tool in veterinary therapeutic field such as tissue engineering. In the present study, equine amnion-derived mesenchymal stromal cells were investigated for applications in veterinary science as an alternative source to bone marrow mesenchymal stem cells and adipose stem cells. Amnion stromal cells isolation and characterization protocol is described; the in vitro cell growth rate was calculated by measuring viable cell number over 20 days. The expression of stem cell markers such as Oct-4, Nanog, Sox-2 and CD105 was assessed by retrotranscription quantitative PCR (RT-qPCR) and differentiation into adipocytes, osteocytes and chondrocytes precursors was analyzed by cytochemical staining. This study showed that amnion stromal cells expressing stem cell markers can differentiate into mesoderm lineage and may be an alternative source to mesenchymal stem cells derived from adipose tissue and bone marrow for the use in tissue repair.

Keywords

EquineAmnionGene expressionDifferentiation

Copyright information

© Springer Science+Business Media B.V. 2011