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Effect of new rotenoid glycoside from the fruits of Amorpha fruticosa LINNE on the growth of human immune cells

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Abstract

A new compound, rotenoid isoflavone glycoside named, 6′-O-β-d-glucopyranosyl-12a-hydroxydalpanol was isolated from the methanolic (MeOH) fruit extract of Amorpha fruticosa LINNE by means of multi-stage column chromatography. Immuno-modulatory activities of this new glycoside were compared with the partitioned fractions of Amorpha fruticosa LINNE. Both of the fractions and purified single compound showed a 19% relatively low cytotoxicity at a maximum concentration of 1.0 g/L in a cultivated normal human lung cell line (HEL299). The purified single compound showed less cytotoxicity than the crude extracts, possibly because residual toxicants were eliminated during purification processes. Cell growth of human T cells was increased by about 15% by adding 0.5 g/L of the fractions compared to the control. Specific production rates of interleukin-6 (IL-6) and tumor necrosis factor (TNF-α) from T cell were higher as 1.16 × 10−4 and 1.86 × 10−4 pg/cell, respectively, in the purified compound, compared to 1.38 × 10−4 and 2.22 × 10−4 pg/cell, respectively, by adding 0.5 g/L of the dichloromethane fraction. Natural killer cell-92MI (NK-92MI) growth supplemented with the supernatant of human T cell was up to 19% higher with the dichloromethane fraction compared with a new single compound at a concentration of 0.5 g/L. Overall, the dichloromethane fraction showed relatively higher immuno-modulatory activities compared with a new single compound, probably due to the synergic effect given by other substances existing in the fractions.

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Abbreviations

NK cell:

Natural killer cell

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Acknowledgements

Special thanks to Mr. Albert Lee who devotedly assisted with technical support. This work was (in part) supported by the Ministry of Commerce, Industry and Energy through the Center for Efficacy Assessment and Development of Functional Foods and Drugs at Hallym University, Korea.

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Authors and Affiliations

  1. Division of Wood Chemistry, Korea Forest Research Institute, Seoul, 130-712, Korea

    Hak Ju Lee & Ha Young Kang

  2. School of Biotechnology and Bioengineering, Kangwon National University, Chunchon, 200-701, Korea

    Cheol Hee Kim, Hyo Sung Kim, Min Chul Kwon & Hyeon Yong Lee

  3. Faculty of Marine Bioscience and Technology, Kangnung National University, Kangnung, 210-702, Korea

    Sang Moo Kim & Il Shik Shin

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  1. Hak Ju Lee
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  2. Ha Young Kang
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  3. Cheol Hee Kim
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  6. Sang Moo Kim
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  8. Hyeon Yong Lee
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Correspondence to Hyeon Yong Lee.

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Lee, H.J., Kang, H.Y., Kim, C.H. et al. Effect of new rotenoid glycoside from the fruits of Amorpha fruticosa LINNE on the growth of human immune cells. Cytotechnology 52, 219–226 (2006). https://doi.org/10.1007/s10616-006-9040-5

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