Article

Cytotechnology

, Volume 46, Issue 1, pp 49-56

Oil red-O stains non-adipogenic cells: a precautionary note

  • Adrienne D. KinkelAffiliated withMuscle Biology Laboratory, Department of Animal Sciences , Washington State University
  • , Melinda  E. FernyhoughAffiliated withMuscle Biology Laboratory, Department of Animal Sciences , Washington State University
  • , Deri L. HelterlineAffiliated withMuscle Biology Laboratory, Department of Animal Sciences , Washington State University
  • , Janet L. VierckAffiliated withMuscle Biology Laboratory, Department of Animal Sciences , Washington State University
  • , Karen S. ObergAffiliated withMuscle Biology Laboratory, Department of Animal Sciences , Washington State University
  • , Tyler J. VanceAffiliated withMuscle Biology Laboratory, Department of Animal Sciences , Washington State University
  • , Gary J. HausmanAffiliated withRichard B. Russell Agricultural Research Center, USDA-ARS
  • , Rodney A. HillAffiliated withDepartment of Animal and Veterinary Science, University of Idaho
  • , Michael V. DodsonAffiliated withMuscle Biology Laboratory, Department of Animal Sciences , Washington State University Email author 

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Abstract

Bovine adipofibroblasts, 3T3-L1 cells, L-6 myogenic cells, and sheep satellite cells were allowed to proliferate for 48 h. Oil red-O (ORO) was dissolved in three different solvents isopropanol, propylene glycol and triethyl phosphate. At 48 h, the proliferative cultures were stained with the three stains. ORO stain prepared in both propylene glycol and triethyl phosphate resulted in bright red droplets appearing in all cultures, whereas ORO dissolved in isopropanol was not taken up by any of the cells. These data suggest that certain preparations of ORO may stain cells in non-adipogenic lineages as well as undifferentiated pre-adipocytes. Caution must be exercised when choosing solvents for ORO in differentiation studies using cells of the fat/adipose lineage.

Keywords

Adipocyte Adipofibroblasts Cell culture Oil red-O Satellite cell Staining