Cytotechnology

, Volume 46, Issue 1, pp 49–56

Oil red-O stains non-adipogenic cells: a precautionary note

Authors

  • Adrienne D. Kinkel
    • Muscle Biology Laboratory, Department of Animal Sciences Washington State University
  • Melinda  E. Fernyhough
    • Muscle Biology Laboratory, Department of Animal Sciences Washington State University
  • Deri L. Helterline
    • Muscle Biology Laboratory, Department of Animal Sciences Washington State University
  • Janet L. Vierck
    • Muscle Biology Laboratory, Department of Animal Sciences Washington State University
  • Karen S. Oberg
    • Muscle Biology Laboratory, Department of Animal Sciences Washington State University
  • Tyler J. Vance
    • Muscle Biology Laboratory, Department of Animal Sciences Washington State University
  • Gary J. Hausman
    • Richard B. Russell Agricultural Research CenterUSDA-ARS
  • Rodney A. Hill
    • Department of Animal and Veterinary ScienceUniversity of Idaho
    • Muscle Biology Laboratory, Department of Animal Sciences Washington State University
Article

DOI: 10.1007/s10616-004-3903-4

Cite this article as:
Kinkel, A.D., Fernyhough, M.E., Helterline, D.L. et al. Cytotechnology (2004) 46: 49. doi:10.1007/s10616-004-3903-4

Abstract

Bovine adipofibroblasts, 3T3-L1 cells, L-6 myogenic cells, and sheep satellite cells were allowed to proliferate for 48 h. Oil red-O (ORO) was dissolved in three different solvents isopropanol, propylene glycol and triethyl phosphate. At 48 h, the proliferative cultures were stained with the three stains. ORO stain prepared in both propylene glycol and triethyl phosphate resulted in bright red droplets appearing in all cultures, whereas ORO dissolved in isopropanol was not taken up by any of the cells. These data suggest that certain preparations of ORO may stain cells in non-adipogenic lineages as well as undifferentiated pre-adipocytes. Caution must be exercised when choosing solvents for ORO in differentiation studies using cells of the fat/adipose lineage.

Keywords

Adipocyte Adipofibroblasts Cell culture Oil red-O Satellite cell Staining

Abbreviation

DMEM

Dulbecco’s modified Eagle’s medium

FBS

Fetal bovine serum

ORO

Oil red-O

PBS

Phosphate-buffered saline, pH 7.08

PEG

Propylene glycol

PSG

Pig skin gelatin

SC

Satellite cell

TEP

Triethyl phosphate

Copyright information

© Springer 2005