Skip to main content

Advertisement

SpringerLink
Log in

Genetic diversity and population genetic structure in three threatened Ocotea species (Lauraceae) from Brazil’s Atlantic Rainforest and implications for their conservation

  • Research Article
  • Published:
Conservation Genetics Aims and scope Submit manuscript

Abstract

The Atlantic Rainforest in Brazil is currently comprised of small fragments due to the history of conversion and degradation in the last five centuries. The rainforest trees, Ocotea catharinensis, O. odorifera and O. porosa have been heavily harvested because of the high economic value of their timber and essential oils. Their respective habitats have undergone substantial reduction in area due to continuing anthropogenic pressures. As a consequence, these species have suffered large declines in population size and are now considered to be potentially vulnerable to extinction. This study investigated the patterns and levels of genetic diversity and inbreeding of these species using eight microsatellite markers in order to define priority populations for conservation management actions focusing on population enhancement and ex situ germplasm collections. High genetic diversity was found for each of the species with moderate genetic differentiation among populations. Most populations displayed significant inbreeding and isolation by distance. The results provide important information to choose priority populations for both in situ and ex situ conservation measures.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Aparicio A, Hampe A, Fernández-Carrillo L, Albaladej RG (2012) Fragmentation and comparative genetic structure of four mediterranean woody species: complex interactions between life-history traits and the landscape context. Divers Distrib 18:226–235

    Article  Google Scholar 

  • BirdLife International (2012) Pipile jacutinga. In: IUCN 2012. IUCN Red List of Threatened Species. Version 2012.2. [www.iucnredlist.org]. Downloaded on March 2013

  • Bittencourt R (2004) Caracterização da Diversidade Genética de Populações Naturais de Ocotea porosa (Lauraceae) no Estado de Santa Catarina. Graduation Monograph, Universidade Federal de Santa Catarina

  • Bohonak AJ (2002) IBD (isolation by distance): a program for analyzes of isolation by distance. J Hered 93(2):153–154

    Article  CAS  PubMed  Google Scholar 

  • Brotto ML, Santos EP, Baitello JB (2009) Lauraceae no morro dos perdidos (Floresta Atlântica), Paraná, Brasil. Rodriguésia 60(2):445–459

    Google Scholar 

  • Carvalho PER (1994) Espécies florestais brasileiras: recomendações silviculturais, potencialidades e uso da madeira. EMBRAPA-CNPF, Brasília, p 640p

    Google Scholar 

  • Carvalho PER (2003) Espécies arbóreas brasileiras. Brasília: embrapa informação tecnológica; Colombo: embrapa florestas, 1:1039 p

  • Carvalho PER (2005) Canela-Sassafrás. Circular técnica. Embrapa CNPF, Brasília, 10:1-12

  • Danieli-Silva A, Varassin IG (2012) Breeding system and thrips (Thysanoptera) pollination in the endangered tree Ocotea porosa (Lauraceae): implications for conservation. Plant Species Biol 28:31–40

    Article  Google Scholar 

  • Daros TL (2006) Sistema reprodutivo e estrutura genética de uma população natural de imbuia (Ocotea porosa) (Nees & C. Mart.) Barroso—Lauraceae). Dissertation, Universidade Federal do Paraná

  • Dick CW (2001) Genetic rescue of remnant tropical trees by an alien pollinator. Proc R Soc Lond 268:2391–2396

    Article  CAS  Google Scholar 

  • Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for a small amount of fresh leaf tissue. Phytochem Bull 19:11–15

    Google Scholar 

  • Earl DA, VonHoldt BM (2012) STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method. Conserv Genet Resour 4(2):359–361

    Article  Google Scholar 

  • Evanno G, Regnaut S, Goudet J (2005) Detecting the number of clusters of individuals using the software Structure: a simulation study. Mol Ecol 14:2611–2620

    Article  CAS  PubMed  Google Scholar 

  • Ferreira ME, Grattapaglia D (1998) Introdução ao uso de marcadores moleculares em análise genética. 3a edn. Brasília, Embrapa CENARGEN, p220

  • Frankham R (2003) Genetics and conservation biology. C.R Biol 326:S22–S29

    Article  PubMed  Google Scholar 

  • Frankham R, Ralls K (1998) Inbreeding leads to extinction. Nature 392(2):441–442

    Article  CAS  Google Scholar 

  • Fuchs EJ, Hamrick JLH (2011) Mating system and pollen flow between remnant populations of the endangered tropical tree, Guaiacum sanctum (Zygophyllaceae). Conserv Genet 12(1):175–185

    Article  Google Scholar 

  • Galetti M, Martuscelli P, Olmos F, Aleixo A (1997) Ecology and conservation of the jacutinga Pipile jacutinga in the Atlantic forest of Brazil. Biol Conserv 82:31–39

    Article  Google Scholar 

  • Gemballa G. (1955) Contribuição para a caracterização da essência de “Ocotea pretiosa Mez” (essência de sassafrás brasileiro). Tese de doutorado. Faculdade Nacional de Farmácia da Universidade do Brasil, Rio de Janeiro, p181

  • Goudet J. (2002) FSTAT: a program to estimate and test gene diversities and fixation indices (version 2.9.3.2). Lausanne: University of Lausanne, Department of Ecology & Evolution

  • Guichoux E, Lagache L, Wagner S, Chaumeil P, Leger P, Lepais O, Lepoittevin C, Malausa T, Revardel E, Salin F, Petit RJ (2011) Current trends in microsatellite genotyping. Mol Ecol Resour 11(4):591–611

    Article  CAS  PubMed  Google Scholar 

  • Hamilton MB (1999) Tropical tree gene flow and seed dispersal. Nature 401:129–130

    Article  CAS  Google Scholar 

  • Hamrick JL, Godt MJW (1996) Effects of life history traits on genetic diversity in plant species. Philoso Trans R Soc B 351:1291–1298

    Article  Google Scholar 

  • Hamrick JL, Loveless MD (1989) The genetic structure of tropical tree populations: associations with reproductive biology. In: Bock JH, Linhart YB (eds) The evolutionary ecology of plants. Westview Press, San Francisco, pp 129–146

    Google Scholar 

  • Hamrick JL, Murawski DA (1991) Levels of allozyme diversity in populations of uncommon neotropical tree species. J Trop Ecol 7:395–399

    Article  Google Scholar 

  • Hamrick JL, Linhart YB, Mitton JB (1979) Relationships between life history characteristics and electrophoretically detectable genetic variation in plants. Annu Rev Ecol Syst 10:173–200

    Article  Google Scholar 

  • Hartl DL, Clark AG (1989) Principles of Population Genetics. Sinauer Associates, Sunderland, Massachusetts, USA. 2ed, p 682

  • INP—Instituto Nacional do Pinho—(1949–1960) Anuário brasileiro de economia florestal. Rio de Janeiro. v. 2–19

  • JBRJ (Instituto de Pesquisas Jardim Botânico do Rio de Janeiro)/Jabot—Banco de Dados da Flora Brasileira. [http://www.jbrj.gov.br/jabot]. Accessed 10 March 2010

  • Kageyama PY, Cunha GC, Barreto KD, Gandara FB, Camargo FRA, Sebbenn AM (2003) Diversidade e autocorrelação genética espacial em populações de Ocotea odorifera (Lauraceae). Sci For 64:108–119

    Google Scholar 

  • Leimu R, Mutikainen P, Koricheva J, Fischer M (2006) How general are positive relationships between plant population size, fitness and genetic variation? J Ecol 94:942–952

    Article  Google Scholar 

  • Lowe AJ, Boshier D, Ward M, Bacles CFE, Navarro C (2005) Genetic resource impacts of habitat loss and degradation; reconciling empirical evidence and predicted theory for neotropical trees. Heredity 95:255–273

    Article  CAS  PubMed  Google Scholar 

  • Machado RD, Souza AH (1948) Esclarecimentos e sugestões sobre o óleo essencial de sassafrás. Anuário Brasileiro de Economia Florestal, Rio de Janeiro. 1(1):206–214

    Google Scholar 

  • Martinelli G, Moraes MA (Orgs) (2013) Livro Vermelho da Flora do Brasil. Andrea Jakobsson, Instituto de Pesquisas Jardim Botânico do Rio de Janeiro, Brasil. 1 edn, p 1102

  • Martins EM, Martinelli G, Arbetman MP, Lamont RW, Simões-Araújo JL, Powell D, Ciampi-Guillardi M, Baldauf C, Quinet A, Galisa P, Shapcott A (2014) Development and characterization of microsatellite loci for three Ocotea species (Lauraceae) threatened with extinction. Gen Mol Res 13(3):5138–5142

  • Mendes SL, de Oliveira MM, Mittermeier RA, Rylands AB. (2008) Brachyteles arachnoides. In: IUCN 2012. IUCN Red List of Threatened Species. Version 2012.2. [www.iucnredlist.org]. Downloaded on March 2013

  • MMA—Ministério do Meio Ambiente (2007) Revisão Áreas Prioritárias para a Conservação da Biodiversidade MMA. Brasília, Brasil

    Google Scholar 

  • MMA—Ministério do Meio Ambiente—(2008) Instrução Normativa no.6 de 23/09/2008, p 55

  • Moraes PLR, Paoli AAS (1999) Morfologia e Estabelecimento de Plântulas de Cryptocarya moschata Nees, Ocotea cathariensis Mez e Endlicheria paniculata (Spreng.) MacBride-Lauraceae. Revista Brasileira de Botânica 22(2):287–295

  • Myers N, Mittermeier RA, Mittermeier CG, Fonseca GAB, Kent J (2000) Biodiversity hotspots for conservation priorities. Nature 403:858–863

    Article  Google Scholar 

  • Nakaoka Sakita M, Yatagai M (1992) Óleo Essencial da Casca de Ocotea catharinensis Mez. (Lauraceae). Anais 2o Congresso Nacional sobre Essências Nativas, p 684–687

  • Oliveira-Filho AT, Scolforo JRS, Mello JM (1994) Composição florística e estrutura comunitária de um remanescente de floresta semidecídua em Lavras, MG. Revista Brasileira de Botânica 17(2):167–182

    Google Scholar 

  • Oltramari AC, Silva JMOD, Pedrotti EL, Maraschin M (2002) Análise Histórica e de Mercado da Atividade Extrativista da Madeira e do Óleo da Canela-Sassafrás (Ocotea odorifera (Vell.) Rohwer) no Estado de Santa Catarina. Revista Árvore 1:99–103

    Google Scholar 

  • Oostermeijer JGB, Luijten SH, den Nijs JCM (2003) Integrating demographic and genetic approaches in plant conservation. Biol Conserv 113:389–398

    Article  Google Scholar 

  • Orloci L (1978) Multivariate analysis in vegetation research. Dr W. Junk B. V, The Hague

    Google Scholar 

  • Peakall R, Smouse PE (2006) GenAlEx 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295

    Article  Google Scholar 

  • Pedroso O, Mattos JR (1987) Estudos sobre Madeiras do Rio Grande do Sul. Publicação do Instituto de Pesquisa de Recursos Naturais Renováveis (IPRNR). Governo do Estado do Rio Grande do Sul 20:61–63

    Google Scholar 

  • Petit RJ, Mousadik AE, Pons O (1998) Identifying populations for conservation on the basis of genetic markers. Conserv Biol 12:844–855

    Article  Google Scholar 

  • Pillar VD, Quadros FLF (1997) Grassland-forest boundaries in southern Brazil. Coenoses 12:119–126

    Google Scholar 

  • Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945–959

    CAS  PubMed Central  PubMed  Google Scholar 

  • Quinet A (2008) O Gênero Ocotea Aubl. (Lauraceae) no Sudeste do Brasil. Thesis, Universidade Federal do Rio de Janeiro

  • Raoul W, Iachan A (1948) Óleo essencial de Sassafrás. Anuário Brasileiro de Economia Florestal, Rio de Janeiro 1(1): 122–127

  • Reitz R, Klein RM, Reis A (1978) Projeto madeira de santa catarina. Sellowia 28(30):1–320

    Google Scholar 

  • Reitz R, Klein RM, Reis A (1988) Projeto madeira do rio grande do sul. Sellowia 34(35):233–239

    Google Scholar 

  • Ribeiro MC, Metzger JP, Martensen AC, Ponzoni F, Hirota MM (2009) Brazilian Atlantic forest: how much is left and how is the remaining forest distributed? Implications for conservation. Biol Conserv 142:1141–1153

    Article  Google Scholar 

  • Rousset F (1997) Genetic differentiation and estimation of gene flow from statistics under isolation by distance. Genetics 145:1219–1228

    CAS  PubMed Central  PubMed  Google Scholar 

  • Shapcott A, Rakotoarinivo M, Smith RJ, Lysaková G, Fay MF, Dransfield J (2007) Can we bring Madagascar’s critically endangered palms back from the brink? Using an understanding on genetics and ecology to guide a conservation and recovery programme for the iconic and critically endangered palm Beccariophoenix madagascariensis. Bot J Linn Soc 154:589–608

    Article  Google Scholar 

  • Silva A, Aguiar IB (1998) Germinação de Sementes de Canela-Preta (Ocotea catharinensis Mez—Lauraceae) sob Diferentes Condições de Luz e Temperatura. Revista do Inst Florest 10(1):17–22

    Google Scholar 

  • Silva A, Aguiar IB, Damião Filho CF, Durigan JF (1998) Caracterização Morfológica e Química de Frutos e Sementes de Canela-Preta (Ocotea catharinensis Mez-Lauraceae). Revista do Inst Florest 10(2):217–228

    Google Scholar 

  • Stefenon VM, Gailing O, Finkeldey R (2007) Genetic structure of Araucaria angustifolia (Araucariaceae) populations in Brazil: implications for the in situ Conservation of genetic resources. Plant Biol. 9:516–525

    Article  CAS  PubMed  Google Scholar 

  • Tarazi R, Montovani A, Reis MS (2010) Fine-scale spatial genetic structure and allozymic diversity in natural populations of Ocotea catharinensis Mez. (Lauraceae). Conserv Genet 11:965–976

    Article  CAS  Google Scholar 

  • Teixeira ML, Barros LM de (1992) Avaliação do Teor de Óleo Essencial da Canela Sassafrás (Ocotea pretiosa (Nees) Mez) na Região do Sul do Estado de Minas Gerais. Anais 2 Congresso Nacional sobre Essências Nativas. Lavras, Minas Gerais

  • Varty N (1998) Ocotea pretiosa. In: IUCN 2011. IUCN red list of threatened species. Version 2011.2. [www.iucnredlist.org]. Accessed 17 April 2012

  • Varty N, Guadagnin DL (1998a) Ocotea catharinensis. In: IUCN 2011. IUCN red list of threatened species. Version 2011.2. [www.iucnredlist.org]. Accessed 17 April 2012

  • Varty N, Guadagnin DL (1998b) Ocotea porosa. In: IUCN 2011. IUCN red list of threatened species. Version 2011.2. [www.iucnredlist.org]. Accessed 17 April 2012

  • Wright S (1965) The interpretation of population structure by F-statistics with special regard to systems of mating. Evolution 19:395–420

    Article  Google Scholar 

  • Efeito da caça sobre abundância de aves e mamíferos na Reserva Biológica do Tinguá, Rio de Janeiro, Brasil. Congresso de Zoologia. Universidade Estadual de Santa Cruz, Ilheús, Bahia

  • Young A, Boyle T, Brown T (1996) The populationg genetic consequences of habitat fragmentation for plants. Tree 11(10):413–418

    CAS  PubMed  Google Scholar 

  • Zalapa JE, Cuevas H, Zhu H, Steffan S, Senalik D, Zeldin E, McCown B, Harbut R, Simon P (2012) Using next-generation sequencing approaches to isolate simple sequence repeat (SSR) loci in the plant sciences. Am J Bot 99(2):193–208

    Article  CAS  PubMed  Google Scholar 

  • Zhu Y, Strassmann JE, Queller DC (2000) Insertions, substitutions, and the origin of microsatellites. Genet Res Camb 76:227–236

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We would like to thank Centro Nacional de Conservação da Flora (CNCFlora) for financial support. Thanks also to the GeneCology Research Centre (University of the Sunshine Coast, Australia) and Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (JBRJ, Rio de Janeiro, Brazil) for technical assistance and the use of their respective laboratory facilities. The first author is grateful for the sandwich scholarship provided by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).

Author information

Authors and Affiliations

  1. Diretoria de Pesquisa, Instituto de Pesquisas Jardim Botânico do Rio de Janeiro, Rua Pacheco Leão 915, Jardim Botânico, Rio de Janeiro, RJ, 22460-030, Brazil

    E. M. Martins, G. Martinelli, C. F. Lira-Medeiros & A. Quinet

  2. Escola Nacional de Botânica Tropical (ENBT), Rio de Janeiro, RJ, Brazil

    E. M. Martins

  3. GeneCology Research Centre, University of the Sunshine Coast, Maroochydore, QLD, Australia

    R. W. Lamont & A. Shapcott

Authors
  1. E. M. Martins
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. W. Lamont
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. Martinelli
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C. F. Lira-Medeiros
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. Quinet
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. Shapcott
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to E. M. Martins.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOC 188 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Martins, E.M., Lamont, R.W., Martinelli, G. et al. Genetic diversity and population genetic structure in three threatened Ocotea species (Lauraceae) from Brazil’s Atlantic Rainforest and implications for their conservation. Conserv Genet 16, 1–14 (2015). https://doi.org/10.1007/s10592-014-0635-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10592-014-0635-7

Keywords

Search

Navigation