Conservation Genetics

, Volume 11, Issue 3, pp 795–802

Isolation by landscape in populations of a prized edible mushroom Tricholoma matsutake

Authors

    • Department of Plant and Microbial BiologyUniversity of California Berkeley
  • Matteo Garbelotto
    • Department of Environmental ScienceUniversity of California Berkeley, Policy and Management
  • Zhendong Fang
    • Shangri-La Alpine Botanical Garden
  • Sterling Keeley
    • Department of BotanyUniversity of Hawaii at Manoa
Research Article

DOI: 10.1007/s10592-009-9894-0

Cite this article as:
Amend, A., Garbelotto, M., Fang, Z. et al. Conserv Genet (2010) 11: 795. doi:10.1007/s10592-009-9894-0

Abstract

Tricholoma matsutake, a wild edible ectomycorrhizal mushroom, is revered for its distinguished flavor and iconic significance. Here, we test for landscape effects on T. matsutake gene flow and population structure in the Eastern Himalayas. Using single-nucleotide polymorphic (SNP) DNA markers, isolation by distance patterns were tested on eight populations within and between watersheds. We find that high, treeless ridgelines are effective barriers to gene flow, even at distances less than 65 km, whereas populations located within watersheds are structured at greater distances. Mantel tests demonstrated a significant positive correlation between Fst and a “landscape distance” measured as the shortest distance between population pairs below treeline r = 0.574, P = 0.002, whereas strict euclidian distances do not correlate. AMOVA analysis revealed significant partitioning with 91% of the genetic variance found within populations and 7% found between watersheds, indicative of sexually recombining populations with limited gene flow between watersheds. We show that landscape is an important determinant of air-dispersed ectomycorrhizal species population structure in heterogeneous landscapes.

Keywords

MatsutakePopulation structureIsolation by distanceEctomycorrhizaEastern HimalayaLandscape genetics

Copyright information

© Springer Science+Business Media B.V. 2009