, Volume 8, Issue 5, pp 1239-1241
Date: 14 Dec 2006

Characterization of 37 microsatellite loci in mountain lions (Puma concolor) for use in forensic and population applications

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The primary uses of these microsatellite markers will be to aid wildlife forensics investigators in the protection of California’s mountain lions from poaching pressures and in the identification of the offending animal involved in attacks on humans. In addition, these loci will be useful in quantifying genetic relationships between and within the different geographic regions of California for wildlife management purposes and should also be of use to researchers in the many other states and countries where mountain lions exist.

Microsatellite loci were isolated using a proprietary magnetic bead-based enrichment procedure that selects for genomic DNA restriction fragments containing [AAAG]n, [CATC]n, [TACA]n, and [TAGA]n, repeat motifs, as described in detail in Jones et al. (2000). Briefly, high molecular weight DNA was extracted from muscle tissue using chloroform/phenol/isopropanol extraction (Sambrook et al. 1989). The DNA was incompletely digested with a cocktail of seven blunt-end ...