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Homoeology of Thinopyrum junceum and Elymus rectisetus chromosomes to wheat and disease resistance conferred by the Thinopyrum and Elymus chromosomes in wheat

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Abstract

Thirteen common wheat “Chinese Spring” (CS)-Thinopyrum junceum addition lines and three common wheat “Fukuhokomuji”(Fuku)-Elymus rectisetus addition lines were characterized and verified as disomic additions of a Th. junceum or E. rectisetus chromosome in the wheat backgrounds by fluorescent genomic in situ hybridization. Another Fuku-E. rectisetus addition line, A1048, was found to contain multiple segregating E. rectisetus chromosomes. Seven partial CS-Th. junceum amphiploids were identified to combine 12–16 Th. junceum chromosomes with CS wheat chromosomes. The disomic addition lines AJDAj5, 7, 8, 9, and HD3508 were identified to contain a Th. junceum chromosome in homoeologous group 1. Two of them, AJDAj7 and AJDAj9, had the same Th. junceum chromosome. AJDAj2, 3, and 4 contained a Th. junceum chromosome in group 2, HD3505 in group 4, AJDAj6 and AJDAj11 in group 5, and AJDAj1 probably in group 6. The disomic addition lines A1026 and A1057 were identified to carry an E. rectisetus chromosome in group 1 and A1034 in group 5. E. rectisetus chromosomes in groups 1–6 were detected in A1048. The homoeologous group of the Th. junceum chromosome in HD3515 could not be determined in this study. Several Th. junceum and E. rectisetus chromosomes in the addition lines were found to contain genes for resistance to Fusarium head blight, tan spot, Stagonospora nodorum blotch, and stem rust (Ug99 races). Understanding of the homoeology of the Th. junceum and E. rectisetus chromosomes with wheat will facilitate utilization of the favorable genes on these alien chromosomes in wheat improvement.

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Abbreviations

FGISH:

Fluorescent genomic in situ hybridization

FITC:

Fluorescein isothiocyanate

RFLPs:

Restriction fragment length polymorphisms

SDS–PAGE:

Sodium dodecyl sulfate–polyacrylamide gel electrophoresis

A-PAGE:

Acid-polyacrylamide gel electrophoresis

HMW:

High molecular weight glutenin subunits

LMW:

Low molecular weight glutenin subunits

FHB:

Fusarium head blight

PIS:

Percentage of infected spike

NIS:

Number of infected spike

SNB:

Stagonospora nodorum blotch

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Acknowledgments

We thank members of the Cai and Xu labs for their help in this research and Drs. Lili Qi and G. Francois Marais for their critical review of this manuscript. Also, we would like to thank Drs. Mark Sorrells and Shahryar Kianian for maintaining and generously providing the RFLP probes for this study. This research was supported by the US Wheat & Barley Scab Initiative and North Dakota Wheat Commission.

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Authors and Affiliations

  1. Department of Plant Sciences, North Dakota State University, Fargo, ND, 58108, USA

    Rachel I. McArthur, Xianwen Zhu, Rebekah E. Oliver & Xiwen Cai

  2. USDA-ARS, Northern Crop Science Laboratory, Fargo, ND, 58102, USA

    Daryl L. Klindworth & Steven S. Xu

  3. Department of Plant Pathology, North Dakota State University, Fargo, ND, 58108, USA

    Robert W. Stack

  4. USDA-ARS, Forage and Range Research Laboratory, Logan, UT, 84322, USA

    Richard R.-C. Wang

Authors
  1. Rachel I. McArthur
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  2. Xianwen Zhu
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  3. Rebekah E. Oliver
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  4. Daryl L. Klindworth
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  5. Steven S. Xu
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  6. Robert W. Stack
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  7. Richard R.-C. Wang
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  8. Xiwen Cai
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Correspondence to Xiwen Cai.

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Responsible Editor: Jiming Jiang

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McArthur, R.I., Zhu, X., Oliver, R.E. et al. Homoeology of Thinopyrum junceum and Elymus rectisetus chromosomes to wheat and disease resistance conferred by the Thinopyrum and Elymus chromosomes in wheat. Chromosome Res 20, 699–715 (2012). https://doi.org/10.1007/s10577-012-9307-y

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