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Ginkgo Biloba Extract Enhances Differentiation and Performance of Neural Stem Cells in Mouse Cochlea

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Abstract

Ginkgo biloba extract (GBE) has been widely used for treatment of neural damage and disorders. Neural stem cells (NSCs) hold promise as a treatment of hearing loss caused by neural damage. However, the biological functions of GBE in modulating NSC behaviors in the cochlea are still largely elusive. In this study, we sought to explore the effects of GBE on the differentiation and performance of NSCs from mouse cochlea. Our data showed that GBE treatment promotes cell survival and NSC proliferation. In addition, GBE treatment also increases NSC differentiation to neurons and enhances the performance of mature neural networks evident by the increased frequency of calcium oscillation. Moreover, neurite outgrowth is also dramatically increased upon GBE treatment. Overall, our study demonstrates the positive regulatory role of GBE in NSC proliferation and differentiation into functional neurons in vitro, supporting the potential therapeutic use of GBE in hearing loss recovery.

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Acknowledgments

This work was funded by the National Natural Science Foundation of China, General Program (no. 81371093).

Conflict of interest

Congpin Wang and Zhao Han declare that they have no conflict of interest.

Ethical approval

All applicable international, national and/or institutional guidelines for the care and use of animals were followed.

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Authors and Affiliations

  1. Department of Pharmacy, Eye and ENT Hospital of Fudan University, 83 Fenyang Road, Shanghai, 200031, China

    Congpin Wang

  2. E.N.T. Department, Eye and ENT Hospital of Fudan University, 83 Fenyang Road, Shanghai, 200031, China

    Zhao Han

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  1. Congpin Wang
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  2. Zhao Han
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Correspondence to Zhao Han.

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Wang, C., Han, Z. Ginkgo Biloba Extract Enhances Differentiation and Performance of Neural Stem Cells in Mouse Cochlea. Cell Mol Neurobiol 35, 861–869 (2015). https://doi.org/10.1007/s10571-015-0180-z

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  • DOI: https://doi.org/10.1007/s10571-015-0180-z

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