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Comparison of Alpha-Synuclein Immunoreactivity in the Hippocampus Between the Adult and Aged Beagle Dogs

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Abstract

Alpha-synuclein (α-syn), as a neuroprotein, is expressed in neural tissue, and it is related to a synaptic transmission and neuronal plasticity. In this study, we compared the distribution and immunoreactivity of α-syn and related gliosis in hippocampus between young adult (2–3 years) and aged (10–12 years) beagle dogs. In both groups, α-syn immunoreactivity was detected in neuropil of all the hippocampal sub-regions, but not in neuronal somata. In the aged hippocampus, α-syn immunoreactivity was apparently increased in mossy fibers compared to that in the adult dog. In addition, α-syn protein level was markedly increased in the aged hippocampus. On the other hand, GFAP and Iba-1 immunoreactivity in astrocytes and microglia, respectively, were increased in all the hippocampal sub-regions of the aged group compared to that in the adult group: especially, their immunoreactivity was apparently increased around mossy fibers. In addition, in this study, we could not find any expression of α-syn in astrocytes and microglia. These results indicate that α-syn immunoreactivity apparently increases in the aged hippocampus and that GFAP and Iba-1 immunoreactivity are also apparently increased at the regions with increased α-syn immunoreactivity. This increase in α-syn expression might be a feature of normal aging.

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Acknowledgments

The authors would like to thank Mr. Seung Uk Lee for their technical help in this study. This work was supported by and by the Technology Innovation Program funded by the Ministry of Knowledge Economy (MKE, Korea), and by the Regional Core Research Program funded by the Korea Ministry of Education, Science and Technology (Medical & Bio-material Research Center).

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Authors and Affiliations

  1. Laboratory of Neuroscience, Department of Physical Therapy, College of Rehabilitation Science, Daegu University, Gyeongsan, 712-714, South Korea

    Ji Hyeon Ahn & Jin Sang Kim

  2. Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, 200-701, South Korea

    Joon Ha Park, Bing Chun Yan, Jae-Chul Lee & Moo-Ho Won

  3. Department of Anatomy, College of Veterinary Medicine, Kangwon National University, Chuncheon, 200-701, South Korea

    Jung Hoon Choi

  4. Department of Anatomy and Physiology, College of Pharmacy, Dankook University, Cheonan, 330-714, South Korea

    Choong Hyun Lee

  5. Department of Oral Anatomy, College of Dentistry, Research Institute of Oral Sciences, Gangneung-Wonju National University, Gangneung, 210-702, South Korea

    Ki-Yeon Yoo

  6. Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, South Korea

    In Koo Hwang

  7. Department of Physiology, Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University, Chuncheon, 200-702, South Korea

    Hyung-Cheul Shin

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  1. Ji Hyeon Ahn
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Corresponding authors

Correspondence to Hyung-Cheul Shin or Moo-Ho Won.

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Ji Hyeon Ahn and Joon Ha Park contributed equally to this study.

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Ahn, J.H., Park, J.H., Yan, B.C. et al. Comparison of Alpha-Synuclein Immunoreactivity in the Hippocampus Between the Adult and Aged Beagle Dogs. Cell Mol Neurobiol 33, 75–84 (2013). https://doi.org/10.1007/s10571-012-9873-8

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  • DOI: https://doi.org/10.1007/s10571-012-9873-8

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