Cellular and Molecular Neurobiology

, Volume 29, Issue 1, pp 55–67

DIXDC1 Promotes Retinoic Acid-Induced Neuronal Differentiation and Inhibits Gliogenesis in P19 Cells

Authors

  • Xiao-Tang Jing
    • Department of Brain Protection & Plasticity ResearchBeijing Institute of Basic Medical Sciences
  • Hai-Tao Wu
    • Department of Brain Protection & Plasticity ResearchBeijing Institute of Basic Medical Sciences
  • Yan Wu
    • Department of Brain Protection & Plasticity ResearchBeijing Institute of Basic Medical Sciences
  • Xin Ma
    • Department of Brain Protection & Plasticity ResearchBeijing Institute of Basic Medical Sciences
  • Shu-Hong Liu
    • Department of Brain Protection & Plasticity ResearchBeijing Institute of Basic Medical Sciences
  • Yan-Rui Wu
    • Department of Brain Protection & Plasticity ResearchBeijing Institute of Basic Medical Sciences
  • Xue-Feng Ding
    • Department of Brain Protection & Plasticity ResearchBeijing Institute of Basic Medical Sciences
  • Xiao-Zhong Peng
    • State Key Lab of Biochemistry & Molecular Biology, Institute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical College
  • Bo-Qin Qiang
    • State Key Lab of Biochemistry & Molecular Biology, Institute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical College
  • Jian-Gang Yuan
    • State Key Lab of Biochemistry & Molecular Biology, Institute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical College
    • Department of Brain Protection & Plasticity ResearchBeijing Institute of Basic Medical Sciences
    • Department of Brain Protection & Plasticity ResearchBeijing Institute of Basic Medical Sciences
Original Paper

DOI: 10.1007/s10571-008-9295-9

Cite this article as:
Jing, X., Wu, H., Wu, Y. et al. Cell Mol Neurobiol (2009) 29: 55. doi:10.1007/s10571-008-9295-9

Abstract

Human DIXDC1 is a member of Dishevelled-Axin (DIX) domain containing gene family which plays important roles in Wnt signaling and neural development. In this report, we first confirmed that expression of Ccd1, a mouse homologous gene of DIXDC1, was up-regulated in embryonic developing nervous system. Further studies showed that Ccd1 was expressed specifically in neurons and colocalized with early neuronal marker Tuj1. During the aggregation induced by RA and neuronal differentiation of embryonic carcinoma P19 cells, expressions of Ccd1 as well as Wnt-1 and N-cadherin were dramatically increased. Stable overexpression of DIXDC1 in P19 cells promoted the neuronal differentiation. P19 cells overexpressing DIXDC1 but not the control P19 cells could differentiate into Tuj1 positive cells with RA induction for only 2 days. Meanwhile, we also found that overexpression of DIXDC1 facilitated the expression of Wnt1 and bHLHs during aggregation and differentiation, respectively, while inhibited gliogenesis by down-regulating the expression of GFAP in P19 cells. Thus, our finding suggested that DIXDC1 might play an important role during neurogenesis, overexpression of DIXDC1 in embryonic carcinoma P19 cells promoted neuronal differentiation, and inhibited gliogenesis induced by retinoic acid.

Keywords

DIXDC1 Ccd1 P19 Cells Retinoic acid Neuronal differentiation Gliogenesis

Supplementary material

10571_2008_9295_MOESM1_ESM.eps (162 kb)
MOESM1 [Identification of the expression of Ccd1 in mouse multiple tissues by Western blot. Nine adult mice tissues were isolated and disassociated with lysis buffer. Expression of Ccd1 was detected by Western blot analysis. A major band about 53 kDa and a weak band about 40 kDa were both detected which appeared to correspond to Ccd1B and Ccd1C protein, respectively.] (EPS 162 kb)
10571_2008_9295_MOESM2_ESM.eps (310 kb)
MOESM2 [Overexpression of DIXDC1 facilitates the expression of Wnt1a during RA-induced aggregation in P19 cells. Two selected DIXDC1 overexpression clones and pcDNA4-P19 control cells were induced with RA for 4 days to aggregate. RNA at different aggregation stages is isolated and analyzed. RNA expression of exogenous DIXDC1, endogenous Ccd1, and Wnt1a were detected by reverse transcription PCR. Besides, Zeocin resistance gene contained in pcDNA4 vector was also amplified to confirm the positive clones.] (EPS 310 kb)

Copyright information

© Springer Science+Business Media, LLC 2008