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Analytical performance of paper electro-biosensor detection platform for point-of-care diagnosis

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Abstract

A whole paper electro-biosensor detection platform for detecting glucose is reported. Glucose oxidase was immobilized on paper-based screen-printed carbon electrodes. The developed biosensor has an excellent electrochemical characteristic and high heterogeneous electron transfer rate constant (ks = 4.23 × 10−4). This new detection platform exhibited good amperometric responses toward glucose, with a wide linear range up to 10 mM with R2 is 0.9973 and the sensitivity is 2.07 μA mM−1. The parallel measurements of glucose by different biosensors had a low relative standard deviation of 4.26 % and demonstrated it had good reproducibility. In the interference studies, there was no significant difference with ascorbic acid or uric acid. For stability, it retained 93.9 % of its initial response after a storage period of 31 days in 4 °C. Besides, the Parkes error grid analysis was indicated that paper-based electrochemical biosensor has a good accuracy on the glucose detection.

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Acknowledgments

This work was supported by the State Key Project of Fundamental Research (Grant 2014CB931900), UCAS Young teacher research fund (Grant Y55103NY00, Y55103EY00 and Y25102TN00).

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Authors and Affiliations

  1. College of Materials Science and Opto-Electronic Technology, University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing, 100049, China

    ChangBing Tao, Ching-Shu Yen & Jen-Tsai Liu

  2. School of Electronic, Electrical and Communication Engineering, University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing, 100049, China

    Ching-Jung Chen

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  1. ChangBing Tao
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  2. Ching-Shu Yen
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  3. Jen-Tsai Liu
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  4. Ching-Jung Chen
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Correspondence to Jen-Tsai Liu or Ching-Jung Chen.

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Tao, C., Yen, CS., Liu, JT. et al. Analytical performance of paper electro-biosensor detection platform for point-of-care diagnosis. Cellulose 23, 3799–3808 (2016). https://doi.org/10.1007/s10570-016-1046-3

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  • DOI: https://doi.org/10.1007/s10570-016-1046-3

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