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Production of single-cell protein with two-step fermentation for treatment of potato starch processing waste

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Abstract

Potato starch processing waste is causing serious environmental problems. This study aimed to convert potato starch processing waste into single-cell protein as high-quality feed using a two-step fermentation process. The mutant strain Aspergillus niger H3 was selected after UV irradiation and ethyl methyl sulfone mutagenesis for more cellulase production. The activities of sodium carboxymethyl cellulase and filter paperase of strain H3 were 8.86 and 4.79 U, respectively, which were much higher than the parent strain (1.18 and 0.62 U). After treatment with strain H3, the cellulose degradation rate of potato residue was 80.54 %. A liquid fermentation using Bacillus licheniformis was performed as the second step. The optimized fermentation conditions were temperature of 32.8 °C, pH 6.67, and inoculum concentration of 1.78 % using the response surface method. Results of this study showed a potential application in large-scale industrial conversion.

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Abbreviations

CMCase:

Sodium carboxymethyl cellulase

CMC-Na:

Sodium carboxymethyl cellulose

CP:

Crude protein

CCD:

Central composite design

D:

Diameter of the colony

EMS:

Ethyl methyl sulfone

FPase:

Filter paperase

H:

Diameter of the halo

PDA:

Potato dextrose agar

RSM:

Response surface method

SCP:

Single-cell protein

TP:

True protein

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Acknowledgments

The authors wish to thank the Chinese government for the financial support of this study under the National High Technology Research and Development Program 2012AA021404 and Heilongjiang Province Technological Project GA08C201.

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Authors and Affiliations

  1. Bioengineering Center, School of Life Science and Technology, Harbin Institute of Technology, Harbin, 150001, People’s Republic of China

    Bingnan Liu, Ying Li, Jinzhu Song, Lihong Zhang, Jiacheng Dong & Qian Yang

  2. State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150090, People’s Republic of China

    Qian Yang

Authors
  1. Bingnan Liu
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  2. Ying Li
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  3. Jinzhu Song
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  4. Lihong Zhang
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  5. Jiacheng Dong
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  6. Qian Yang
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Corresponding author

Correspondence to Qian Yang.

Additional information

Bingnan Liu and Ying Li have contributed equally to this work.

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Liu, B., Li, Y., Song, J. et al. Production of single-cell protein with two-step fermentation for treatment of potato starch processing waste. Cellulose 21, 3637–3645 (2014). https://doi.org/10.1007/s10570-014-0400-6

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