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Lutein protects against β-amyloid peptide-induced oxidative stress in cerebrovascular endothelial cells through modulation of Nrf-2 and NF-κb

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Abstract

In the present study, we determined the protective role of lutein against Aβ 25–35 peptide-induced oxidative stress and apoptosis in bEND.3 cells. Cell viability was determined through MTT assay. Reactive oxygen species, lipid peroxides, and antioxidant enzyme activities were evaluated to analyze the oxidative stress status. NF-κB and Nrf-2 downstream target protein expressions were determined through western blot. Apoptosis was analyzed through caspase activities and subG1 accumulation. The results showed that Aβ 25–35 significantly increased (p < 0.001) oxidative stress biomarkers. Aβ 25–35 significantly up-regulated NF-κB nuclear expression and down-regulated Nrf-2 levels and HO-1 and, NQO-1 expressions. Aβ 25–35 induced apoptosis through decreasing mitochondrial membrane potential and increasing caspase 9 and 3 activities. Lutein pre-treatment significantly (p < 0.001) improved cell viability and decreased ROS levels (p < 0.001) and lipid peroxidation (p < 0.01). Lutein prevented Aβ 25–35-induced NF-κB nuclear expressions and up-regulated Nrf-2 expressions. Further, lutein also improved mitochondrial membrane potential and down-regulated caspase activities and subG1 accumulation. The present study shows the protective role of lutein against Aβ 25–35-induced toxicity by modulating Nrf-2 and NF-κB expressions in cerebrovascular endothelial cells.

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Authors and Affiliations

  1. Department of Neurology, Tianjin Nankai Hospital, No.122, Sanwei Road, Nankai District, Tianjin, 300100, People’s Republic of China

    Tao Liu, Jin-sheng Zhao, Fan-zheng Meng & Heng Wang

  2. Department of Rehabilitation, Tianjin Fourth Central Hospital, Tianjin, 300140, China

    Wei-hong Liu

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  1. Tao Liu
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  2. Wei-hong Liu
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  3. Jin-sheng Zhao
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  4. Fan-zheng Meng
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  5. Heng Wang
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Correspondence to Tao Liu.

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Liu, T., Liu, Wh., Zhao, Js. et al. Lutein protects against β-amyloid peptide-induced oxidative stress in cerebrovascular endothelial cells through modulation of Nrf-2 and NF-κb. Cell Biol Toxicol 33, 57–67 (2017). https://doi.org/10.1007/s10565-016-9360-y

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  • DOI: https://doi.org/10.1007/s10565-016-9360-y

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