Abstract
In this study we evaluated the biocompatibility of a modified polyurethane nanofiber membrane on a polypropylene spunbond substrate. This material was treated with plasma using diffuse coplanar surface barrier discharge, and subsequent modification was done by continuous spraying of a biologically active chitosan solution (CHIT) containing an inclusion complex of β-cyclodextrin (β-CD) encapsulating berberine (BRB). Biocompatibility was evaluated using several in vitro assays. Human dermal fibroblasts (HDFs) and 3T3 murine fibroblasts were used as biological models. The results of these assays showed that a polyurethane nanofiber membrane modified by CHIT/β-CD/BRB appears to be non-toxic and biocompatible; potentially, it could be used as a wound dressing after further testing.
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References
Akçakoca Kumbasar EP, Akduman Ç, Çay A (2014) Effects of β-cyclodextrin on selected properties of electrospun thermoplastic polyurethane nanofibres. Carbohydr Polym 104:42–49. doi:10.1016/j.carbpol.2013.12.065
Archana D, Dutta J, Dutta PK (2013) Evaluation of chitosan nano dressing for wound healing: characterization, in vitro and in vivo studies. Int J Biol Macromol 57:193–203. doi:10.1016/j.ijbiomac.2013.03.002
Azuma K, Izumi R, Osaki T, Ifuku S, Morimoto M, Saimoto H, Minami S, Okamoto Y (2015) Chitin, chitosan, and its derivatives for wound healing: old and new materials. J Funct Biomater 6:104–142. doi:10.3390/jfb6010104
Boateng JS, Matthews KH, Stevens HN, Eccleston GM (2008) Wound healing dressings and drug delivery systems: a review. J Pharm Sci 97:2892–2923. doi:10.1002/jps.21210
Bodiwala HS, Sabde S, Mitra D, Bhutani KK, Singh IP (2011) Synthesis of 9-substituted derivatives of berberine as anti-HIV agents. Eur J Med Chem 46:1045–1049. doi:10.1016/j.ejmech.2011.01.016
Černák M, Černáková Ľ, Hudec I, Kováčik D, Zahoranová A (2009) diffuse coplanar surface barrier discharge and its applications for in-line processing of low-added-value materials. Eur Phys J Appl Phys 47:22806. doi:10.1051/epjap/2009131
Chen JP, Chang GY, Chen JK (2008) Electrospun collagen/chitosan nanofibrous membrane as wound dressing. Colloids Surf A Physicochem Eng Asp 313:183–188. doi:10.1016/j.colsurfa.2007.04.129
Cherng JY, Hou TY, Shih MF, Talsma H, Hennink WE (2013) Polyurethane-based drug delivery systems. Int J Pharm 450:145–162. doi:10.1016/j.ijpharm.2013.04.063
Dai T, Tanaka M, Huang YY, Hamblin MR (2011) Chitosan preparations for wounds and burns: antimicrobial and wound-healing effects. Expert Rev Anti Infect Ther 9:857–879. doi:10.1586/eri.11.59
Dragúňová J, Kabát P, Koller J (2013) Skin explant cultures as a source of keratinocytes for cultivation. Cell Tissue Bank 14:317–324. doi:10.1007/s10561-012-9330-4
Enoch S, Leaper DJ (2008) Basic science of wound healing. Surgery (Oxford) 26:31–37. doi:10.1016/j.mpsur.2007.11.005
Giordano F, Novak C, Moyano JR (2001) Thermal analysis of cyclodextrins and their inclusion compounds. Thermochim Acta 380:123–151. doi:10.1016/S0040-6031(01)00665-7
Hazra S, Hossain M, Kumar GS (2014) Studies on α-, β-, and γ-cyclodextrin inclusion complexes of isoquinoline alkaloids berberine, palmatine and coralyne. J Incl Phenom Macrocycl Chem 78:311–323. doi:10.1007/s10847-013-0301-6
Ignatius AA, Claes L (1996) In vitro biocompatibility of bioresorbable polymers: poly (l, dl-lactide) and poly (l-lactide–co-glycolide). Biomaterials 17:831–839. doi:10.1016/0142-9612(96)81421-9
Ignatius AA, Schmidt C, Kaspar D, Claes LE (2001) In vitro biocompatibility of resorbable experimental glass ceramics for bone substitutes. J Biomed Mater Res 55:285–294. doi:10.1002/1097-4636(20010605)55:3<285:AID-JBM1016>3.0.CO;2-1
Jayakumar R, Prabaharan M, Sudheesh Kumar PT, Nair SV, Tamura H (2011) Biomaterials based on chitin and chitosan in wound dressing applications. Biotechnol Adv 29:322–337. doi:10.1016/j.biotechadv.2011.01.005
Jia B, Li Y, Wang D, Duan R (2014) Study on the interaction of β-Cyclodextrin and Berberine hydrochloride and its analytical application. PLoS ONE 9:e95498. doi:10.1371/journal.pone.0095498
Kara F, Ayse Aksoy E, Yuksekdag Z, Hasirci N, Aksoy S (2014) Synthesis and surface modification of polyurethanes with chitosan for antibacterial properties. Carbohyd Polym 112:39–47. doi:10.1016/j.carbpol.2014.05.019
Kara F, Ayse Aksoy E, Yuksekdag Z, Aksoy S, Hasirci N (2015) Enhancement of antibacterial properties of polyurethanes by chitosan and heparin immobilization. Appl Surf Sci 357:1692–1702. doi:10.1016/j.apsusc.2015.09.227
Khil MS, Cha DI, Kim HY, Kim IS, Bhattarai N (2003) Electrospun nanofibrous polyurethane membrane as wound dressing. J Biomed Mater Res B Appl Biomater 67:675–679. doi:10.1002/jbm.b.10058
Küpeli E, Koşar M, Yeşilada E, Hüsnü K, Başer C, Başer C (2002) A comparative study on the anti-inflammatory, antinociceptive and antipyretic effects of isoquinoline alkaloids from the roots of Turkish Berberis species. Life Sci 72:645–657. doi:10.1016/S0024-3205(02)02200-2
Li N, Xu L (2010) Thermal analysis of β-cyclodextrin/Berberine chloride inclusion compounds. Thermochim Acta 499:166–170. doi:10.1016/j.tca.2009.10.014
Lv W, Luo J, Deng Y, Sun Y (2013) Biomaterials immobilized with chitosan for rechargeable antimicrobial drug delivery. J Biomed Mater Res A 101A:447–455. doi:10.1002/jbm.a.34350
Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63. doi:10.1016/0022-1759(83)90303-4
Park KD, Lee JH, Kim SH, Kang TH, Moon JS, Kim SU (2006) Synthesis of 13-(substituted benzyl) berberine and berberrubine derivatives as antifungal agents. Bioorg Med Chem Lett 16:3913–3916. doi:10.1016/j.bmcl.2006.05.033
Patrulea V, Ostafe V, Borchard G, Jordan O (2015) Chitosan as a starting material for wound healing applications. Eur J Pharm Biopharm 97(Pt B):417–426. doi:10.1016/j.ejpb.2015.08.004
Samosorn S, Tanwirat B, Muhamad N, Casadei G, Tomkiewicz D, Lewis K, Suksamrarn A, Prammananan T, Gornall KC, Beck JL, Bremner JB (2009) Antibacterial activity of berberine-NorA pump inhibitor hybrids with a methylene ether linking group. Bioorg Med Chem 17:3866–3872. doi:10.1016/j.bmc.2009.04.028
Schmalz G (1988) Agar overlay method. Int Endod J 21:59–66. doi:10.1111/j.1365-2591.1988.tb00956.x
Sjögren G, Sletten G, Dahl J (2000) Cytotoxicity of dental alloys, metals, and ceramics assessed by Millipore filter, agar overlay, and MTT tests. J Prosthet Dent 84:229–236. doi:10.1067/mpr.2000.107227
Thakur RA, Florek CA, Kohn J, Michniak BB (2008) Electrospun nanofibrous polymeric scaffold with targeted drug release profiles for potential application as wound dressing. Int J Pharm 364:87–93. doi:10.1016/j.ijpharm.2008.07.033
Vitteková M, Dragúňová J, Kabát P, Božiková M, Bakoš D, Koller J (2014) Cytotoxicity testing of scaffolds potentially suitable for the preparation of three-dimensional skin substitutes. Cell Tissue Bank 15:345–355. doi:10.1007/s10561-013-9390-0
Vojtaššák J, Bakoš D, Danihel Ľ, Krištín J, Böhmer D, Danišovič Ľ, Blaško M (2001) In vitro cytotoxicity testing of coladerm membrane. Cell Tissue Bank 2:225–233. doi:10.1023/A:1021146217176
Vuddanda PR, Chakraborty S, Singh S (2010) Berberine: a potential phytochemical with multispectrum therapeutic activities. Expert Opin Investig Drugs 19:1297–1307. doi:10.1517/13543784.2010.517745
Wang Y, Hong Q, Chen Y, Lian X, Xiong Y (2012) Surface properties of polyurethanes modified by bioactive polysaccharide-based polyelectrolyte multilayers. Colloids Surf B Biointerfaces 100:77–83. doi:10.1016/j.colsurfb.2012.05.030
Yu JS, Wei FD, Gao W, Zhao CC (2002) Thermodynamic study on the effects of β-cyclodextrin inclusion with berberine. Spectrochim Acta Part A 58:249–256. doi:10.1016/S1386-1425(01)00536-4
Zdrahala RJ, Zdrahala IJ (1999) Biomedical applications of polyurethanes: a review of past promises, present realities, and a vibrant future. J Biomater Appl 14:67–90. doi:10.1177/088532829901400104
Acknowledgments
The authors thank Dr. Jacob Bauer for his critical comments on the manuscript and the language editing. This work was supported by the Slovak Research and Development Agency under contract No. APVV 0294-14, by the Slovak Ministry of Education, Science, Research and Sport under contract No: VEGA 1/0297/14 and by Research and Development Operational Programme funded by the ERDF Grant No.: ITMS 26240220086.
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Klempaiová, M., Dragúňová, J., Kabát, P. et al. Cytotoxicity testing of a polyurethane nanofiber membrane modified with chitosan/β-cyclodextrin/berberine suitable for wound dressing application: evaluation of biocompatibility. Cell Tissue Bank 17, 665–675 (2016). https://doi.org/10.1007/s10561-016-9585-2
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DOI: https://doi.org/10.1007/s10561-016-9585-2