Skip to main content

Advertisement

SpringerLink
Log in

Isolation of human dermis derived mesenchymal stem cells using explants culture method: expansion and phenotypical characterization

  • Published:
Cell and Tissue Banking Aims and scope Submit manuscript

Abstract

Recent studies have reported that stem cells can be isolated from a wide range of tissues including bone marrow, fatty tissue, adipose tissue and placenta. Moreover, several studies also suggest that skin dermis could serve as a source of stem cells, but are of unclear phenotype. Therefore, we isolated and investigated to determine the potential of stem cell within human skin dermis. We isolated cells from human dermis, termed here as human dermis-derived mesenchymal stem cells (hDMSCs) which is able to be isolated by using explants culture method. Our method has an advantage over the enzymatic method as it is easier, less expensive and less cell damage. hDMSCs were maintained in basal culture media and proliferation potential was measured. hDMSCs were highly proliferative and successfully expanded with no additional growth factor. In addition, hDMSCs revealed normal karyotype and expressed high levels of CD90, CD73 and CD105 while did not express the surface markers for CD34, CD45 and HLA-DR. Also, we confirmed that hDMSCs possess the capacity to differentiate into multiple lineage including adipocyte, osteocyte, chondrocyte and precursor of hepatocyte lineage. Considering these results, we suggest that hDMSCs might be a valuable source of stem cells and could potentially be a useful source of clinical application.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Crigler L, Kazhanie A, Yoon TJ, Zakhari J, Anders J, Taylor B, Virador VM (2007) Isolation of a mesenchymal cell population from murine dermis that contains progenitors of multiple cell lineages. FASEB J 21:2050–2063

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Dhanasekaran M, Indumathi S, Poojitha R, Kanmani A, Rajkumar JS, Sudarsanam D (2013) Plasticity and banking potential of cultured adipose tissue derived mesenchymal stem cells. Cell Tissue Bank 14:303–315

    Article  CAS  PubMed  Google Scholar 

  • Gao Y, Bai C, Xiong H, Li Q, Shan Z, Huang L, Ma Y, Guan W (2013) Isolation and characterization of chicken dermis-derived mesenchymal stem/progenitor cells. Biomed Res Int 2013:626258

    PubMed Central  PubMed  Google Scholar 

  • Giordano A, Galderisi U, Marino IR (2007) From the laboratory bench to the patient’s bedside: an update on clinical trials with mesenchymal stem cells. J Cell Physiol 211:27–35

    Article  CAS  PubMed  Google Scholar 

  • Herzog EL, Chai L, Krause DS (2003) Plasticity of marrow-derived stem cells. Blood 102:3483–3493

    Article  CAS  PubMed  Google Scholar 

  • Huard J, Cao B, Qu-Petersen Z (2003) Muscle-derived stem cells: potential for muscle regeneration. Birth Defects Res C Embryo Today 69:230–237

    Article  CAS  PubMed  Google Scholar 

  • Kanazawa H, Fujimoto Y, Teratani T, Iwasaki J, Kasahara N, Negishi K, Tsuruyama T, Uemoto S, Kobayashi E (2011) Bone marrow-derived mesenchymal stem cells ameliorate hepatic ischemia reperfusion injury in a rat model. PLoS ONE 6:e19195

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Kheolamai P, Dickson AJ (2009) Liver-enriched transcription factors are critical for the expression of hepatocyte marker genes in mES-derived hepatocyte-lineage cells. BMC Mol Biol 10:35

    Article  PubMed Central  PubMed  Google Scholar 

  • Kim SW, Han H, Chae GT, Lee SH, Bo S, Yoon JH, Lee YS, Lee KS, Park HK, Kang KS (2006) Successful stem cell therapy using umbilical cord blood-derived multipotent stem cells for Buerger’s disease and ischemic limb disease animal model. Stem Cells 24:1620–1626

    Article  PubMed  Google Scholar 

  • Li Y, Chen J, Chen XG, Wang L, Gautam SC, Xu YX, Katakowski M, Zhang LJ, Lu M, Janakiraman N, Chopp M (2002) Human marrow stromal cell therapy for stroke in rat: neurotrophins and functional recovery. Neurology 59:514–523

    Article  CAS  PubMed  Google Scholar 

  • Ogawa R, Mizuno H, Watanabe A, Migita M, Shimada T, Hyakusoku H (2004) Osteogenic and chondrogenic differentiation by adipose-derived stem cells harvested from GFP transgenic mice. Biochem Biophys Res Commun 313:871–877

    Article  CAS  PubMed  Google Scholar 

  • Park JR, Jung JW, Lee YS, Kang KS (2008) The roles of Wnt antagonists Dkk1 and sFRP4 during adipogenesis of human adipose tissue-derived mesenchymal stem cells. Cell Prolif 41:859–874

    Article  CAS  PubMed  Google Scholar 

  • Park SB, Seo KW, So AY, Seo MS, Yu KR, Kang SK, Kang KS (2012) SOX2 has a crucial role in the lineage determination and proliferation of mesenchymal stem cells through Dickkopf-1 and c-MYC. Cell Death Differ 19:534–545

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Pountos I, Corscadden D, Emery P, Giannoudis PV (2007) Mesenchymal stem cell tissue engineering: techniques for isolation, expansion and application. Injury 38(Suppl 4):S23–S33

    Article  PubMed  Google Scholar 

  • Riekstina U, Cakstina I, Parfejevs V, Hoogduijn M, Jankovskis G, Muiznieks I, Muceniece R, Ancans J (2009) Embryonic stem cell marker expression pattern in human mesenchymal stem cells derived from bone marrow, adipose tissue, heart and dermis. Stem Cell Rev 5:378–386

    Article  CAS  PubMed  Google Scholar 

  • Shiojiri N, Lemire JM, Fausto N (1991) Cell lineages and oval cell progenitors in rat liver development. Cancer Res 51:2611–2620

    CAS  PubMed  Google Scholar 

  • Snykers S, De Kock J, Rogiers V, Vanhaecke T (2009) In vitro differentiation of embryonic and adult stem cells into hepatocytes: state of the art. Stem Cells 27:577–605

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Studeny M, Marini FC, Dembinski JL, Zompetta C, Cabreira-Hansen M, Bekele BN, Champlin RE, Andreeff M (2004) Mesenchymal stem cells: potential precursors for tumor stroma and targeted-delivery vehicles for anticancer agents. J Natl Cancer Inst 96:1593–1603

    Article  CAS  PubMed  Google Scholar 

  • Tilghman SM, Belayew A (1982) Transcriptional control of the murine albumin/alpha-fetoprotein locus during development. Proc Natl Acad Sci USA 79:5254–5257

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Toma JG, Akhavan M, Fernandes KJ, Barnabe-Heider F, Sadikot A, Kaplan DR, Miller FD (2001) Isolation of multipotent adult stem cells from the dermis of mammalian skin. Nat Cell Biol 3:778–784

    Article  CAS  PubMed  Google Scholar 

  • Tuli R, Seghatoleslami MR, Tuli S, Wang ML, Hozack WJ, Manner PA, Danielson KG, Tuan RS (2003) A simple, high-yield method for obtaining multipotential mesenchymal progenitor cells from trabecular bone. Mol Biotechnol 23:37–49

    Article  CAS  PubMed  Google Scholar 

  • Vaculik C, Schuster C, Bauer W, Iram N, Pfisterer K, Kramer G, Reinisch A, Strunk D, Elbe-Burger A (2012) Human dermis harbors distinct mesenchymal stromal cell subsets. J Invest Dermatol 132:563–574

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Yen BL, Huang HI, Chien CC, Jui HY, Ko BS, Yao M, Shun CT, Yen ML, Lee MC, Chen YC (2005) Isolation of multipotent cells from human term placenta. Stem Cells 23:3–9

    Article  CAS  PubMed  Google Scholar 

  • Yu BD, Mukhopadhyay A, Wong C (2008) Skin and hair: models for exploring organ regeneration. Hum Mol Genet 17:R54–R59

    Article  CAS  PubMed  Google Scholar 

  • Yu KR, Lee JY, Kim HS, Hong IS, Choi SW, Seo Y, Kang I, Kim JJ, Lee BC, Lee S, Kurtz A, Seo KW, Kang KS (2014) A p38 MAPK-mediated alteration of COX-2/PGE2 regulates immunomodulatory properties in human mesenchymal stem cell aging. PLoS ONE 9:e102426

    Article  PubMed Central  PubMed  Google Scholar 

  • Yucel G, Altindag B, Gomez-Ospina N, Rana A, Panagiotakos G, Lara MF, Dolmetsch R, Oro AE (2013) State-dependent signaling by Cav1.2 regulates hair follicle stem cell function. Genes Dev 27:1217–1222

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, Benhaim P, Lorenz HP, Hedrick MH (2001) Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng 7:211–228

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This research was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2013R1A1A2057787), (NRF-2014R1A2A2A01003737) and iPET (Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries), Ministry of Food, Agriculture, Forestry and Fisheries (112008-5).

Conflict of interest

The authors have declared that there is no conflict of interests.

Author information

Authors and Affiliations

  1. Department of Thoracic and Cardiovascular Surgery, School of Medicine, Kangwon National University, Chuncheon, 200-701, Republic of Korea

    Jeong-Ran Park, Eunjeong Kim, Jungwon Yang, Hanbyeol Lee, Sung-Min Park & Se-Ran Yang

  2. Department of Pulmonary Medicine, School of Medicine, Kangwon National University, Chuncheon, 200-701, Republic of Korea

    Seok-Ho Hong

  3. College of Veterinary Medicine, Kangwon National University, Chuncheon, 200-701, Republic of Korea

    Heung-Myong Woo

  4. School of Medicine, Stem Cell Institute, Kangwon National University, 1 Kangwondaehak-gil, Chuncheon, Gangwon, 200-701, Republic of Korea

    Jeong-Ran Park, Eunjeong Kim, Jungwon Yang, Hanbyeol Lee, Seok-Ho Hong, Heung-Myong Woo, Sung-Min Park & Se-Ran Yang

  5. Institute of Medical Science, Kangwon National University, Chuncheon, 200-701, Republic of Korea

    Jeong-Ran Park

  6. Department of Obstetrics and Gynecology, School of Medicine, Kangwon National University, Chuncheon, 200-701, Republic of Korea

    Sunghun Na

Authors
  1. Jeong-Ran Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Eunjeong Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jungwon Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hanbyeol Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Seok-Ho Hong
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Heung-Myong Woo
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Sung-Min Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Sunghun Na
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Se-Ran Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Se-Ran Yang.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (PPT 599 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Park, JR., Kim, E., Yang, J. et al. Isolation of human dermis derived mesenchymal stem cells using explants culture method: expansion and phenotypical characterization. Cell Tissue Bank 16, 209–218 (2015). https://doi.org/10.1007/s10561-014-9471-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10561-014-9471-8

Keywords

Search

Navigation