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Issues of banking breast cancer cells to generate mammospheres

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Abstract

Identification of breast cancer stem cells, within the context of the tumor tissue, requires an efficient and standardized method to preserve the functional features of living cells. Although isolating cancer stem cells can be laborious and time-consuming, minimal processing may be advantageous for the banking of specimens from which cultures are not immediately needed. Homogenization of banking procedures will result in a reliable network of biorepositories for cooperative studies and several research groups are focusing on the issues of tissue banks for translational medicine. Most tissue banks collect and freeze unprocessed cancer specimens, which cannot therefore be used to generate viable cells. We discuss the principal issues of biobanking breast cancer living cells and protocols for mammospheres formation from single cell suspension of tumor cells.

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Acknowledgments

We declare no conflict of interest. This work was supported by: Progetto BiOnCam, Biobanca Oncologica Campania, CRPO Centro Regionale di Prevenzione Oncologica, Regione Campania, Associazione Italiana per la Ricerca sul Cancro, Ministero dell’Universita` e Ricerca, and Ministero della Salute, Italia.

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Authors and Affiliations

  1. Dipartimento di Biologia e Patologia Cellulare e Molecolare, Università degli Studi di Napoli Federico II, via Pansini 5, 80131, Naples, Italy

    Bianca Maria Veneziani

  2. Dipartimento di Endocrinologia e Oncologia Molecolare e Clinica, Università degli Studi di Napoli Federico II, via Pansini 5, 80131, Naples, Italy

    Sabino De Placido

  3. Oncotech, Facoltà di Medicina e Chirurgia, Università degli Studi di Napoli Federico II, via Pansini 5, 80131, Naples, Italy

    Bianca Maria Veneziani & Sabino De Placido

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  1. Bianca Maria Veneziani
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  2. Sabino De Placido
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Correspondence to Bianca Maria Veneziani.

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Veneziani, B.M., De Placido, S. Issues of banking breast cancer cells to generate mammospheres. Cell Tissue Bank 14, 153–158 (2013). https://doi.org/10.1007/s10561-012-9320-6

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  • DOI: https://doi.org/10.1007/s10561-012-9320-6

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