Abstract
Tissues from 78 musculoskeletal donors were concurrently tested for microorganisms using both a swab and liquid culture method. An aggregate total of 20 organisms were detected by both methods. The swab detected 4/20 organisms while the liquid culture detected 18/20 organisms. The swab method yielded sensitivity and negative predictive values of 20 and 92.3%, respectively. Comparatively, the liquid culture displayed a sensitivity of 90% and a negative predictive value of 99%. These results clearly demonstrate that the liquid culture method is superior to swab cultures in microbial detection. Additional studies are necessary to determine the optimal culture conditions for different types of tissues when utilizing the liquid culture method.
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References
American Association of Tissue Banks (2008) Standards for tissue banking, 12th edn. AATB, Mclean
Eastlund T (2006) Bacterial infection transmitted by human tissue allograft transplantation. Cell Tissue Banking 7(3):147–166
Fideler BM, Vangsness CT Jr, Lu B, Orlando C, Moore T (1995) Gamma irradiation: effects on biomechanical properties of human bone-patellar tendon-bone allografts. Am J Sports Med 23(5):643–646
Mankin HJ, Hornicek FJ, Raskin KA (2005) Infection in massive bone allografts. Clin Ortho Relat Res 432:210–216
Mills A, Roberts M (2001) Evaluation of culturing methods at predicting graft sterility for aseptically processed tissue [Abstract]. In: Proceedings of the 25th Annual Meeting American Association of Tissue Banks; 2001 Aug 25–29; Washington, DC p 49. Abstract S-9
Rasmussen TJ, Feder SM, Butler DL, Noyes FR (1994) The effects of 4 mrad of gamma irradiation on the initial mechanical properties of bone-patellar tendon-bone grafts. Arthroscopy 10(2):188–197
Roberts TS, Drez D Jr, McCarthy W, Paine R (1991) Anterior cruciate ligament reconstruction using freeze-dried, ethylene oxide-sterilized, bone-patellar tendon-bone allografts. Two year results in thirty-six patients. Am J Sports Med 19(1):35–41
Tomford WW, Thongphasuk J, Mankin HJ, Ferraro MJ (1990) Frozen musculoskeletal allografts. A study of the clinical incidence and causes of infection associated with their use. J Bone Joint Surg (American Volume) 72(8):1137–1143
Veen MR, Bloem RM, Petit PL (1994) Sensitivity and negative predictive value of swab cultures in musculoskeletal allograft procurement. Clin Orthop Relat Res 300:259–263
Vehmeyer SB, Bloem RM, Deijkers RL, Veen MR, Petit PL (1999) A comparative study of blood and bone marrow cultures in cadaveric bone donation. J Hosp Infect 43(4):305–308
Vehmeyer SB, Bloem RM, Petit PL (2001) Microbiological screening of post-mortem bone donors—two case reports. J Hosp Infect 47(3):193–197
Wang S, Zinderman C, Wise R, Braun M (2007) Infections and human tissue transplants: review of FDA MedWatch reports 2001–2004. Cell Tissue Banking 8(3):211–219
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Octavio V. Martinez and David C. Landy contributed equally to this study.
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Dennis, J.A., Martinez, O.V., Landy, D.C. et al. A comparison of two microbial detection methods used in aseptic processing of musculoskeletal allograft tissues. Cell Tissue Bank 12, 45–50 (2011). https://doi.org/10.1007/s10561-009-9158-8
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DOI: https://doi.org/10.1007/s10561-009-9158-8