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High-efficient and high-content cytotoxic recording via dynamic and continuous cell-based impedance biosensor technology

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Abstract

Cell-based bioassays were effective method to assess the compound toxicity by cell viability, and the traditional label-based methods missed much information of cell growth due to endpoint detection, while the higher throughputs were demanded to obtain dynamic information. Cell-based biosensor methods can dynamically and continuously monitor with cell viability, however, the dynamic information was often ignored or seldom utilized in the toxin and drug assessment. Here, we reported a high-efficient and high-content cytotoxic recording method via dynamic and continuous cell-based impedance biosensor technology. The dynamic cell viability, inhibition ratio and growth rate were derived from the dynamic response curves from the cell-based impedance biosensor. The results showed that the biosensors has the dose-dependent manners to diarrhetic shellfish toxin, okadiac acid based on the analysis of the dynamic cell viability and cell growth status. Moreover, the throughputs of dynamic cytotoxicity were compared between cell-based biosensor methods and label-based endpoint methods. This cell-based impedance biosensor can provide a flexible, cost and label-efficient platform of cell viability assessment in the shellfish toxin screening fields.

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Acknowledgments

This work was supported by NSFC (No.61320106002, No.81501553), High-level Project of Cooperation Improvement of Sino-American and Oceania Region of Ministry of Education of China (No.20152026), and China Postdoctoral Science Foundation (No. 2015 M570511).

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Authors and Affiliations

  1. Key Laboratory of Biomedical Engineering of Ministry of Education, Biosensor National Special Laboratory, Department of Biomedical Engineering, Zhejiang University, Hangzhou, Zhejiang, 310027, China

    Ning Hu, Jiaru Fang, Yuxiang Pan, Kaiqi Su, Xi Zhang & Ping Wang

  2. State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai, 200050, China

    Ning Hu & Ping Wang

  3. Division of Biomedical Engineering, Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Cambridge, MA, 02139, USA

    Ning Hu

  4. Harvard-MIT Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA

    Ning Hu

  5. State Key Laboratory of Diagnostic and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Disease, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310006, China

    Ling Zou

  6. Department of Electrical and Computer Engineering, Michigan State University, East Lansing, MI, 48824, USA

    Hao Wan

Authors
  1. Ning Hu
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  2. Jiaru Fang
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  3. Ling Zou
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  4. Hao Wan
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  5. Yuxiang Pan
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  6. Kaiqi Su
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  7. Xi Zhang
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  8. Ping Wang
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Correspondence to Ning Hu.

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Hu, N., Fang, J., Zou, L. et al. High-efficient and high-content cytotoxic recording via dynamic and continuous cell-based impedance biosensor technology. Biomed Microdevices 18, 94 (2016). https://doi.org/10.1007/s10544-016-0118-3

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  • DOI: https://doi.org/10.1007/s10544-016-0118-3

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