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Metanephrine neuroendocrine tumor marker detection by SERS using Au nanoparticle/Au film sandwich architecture

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Abstract

Neuroendocrine tumors, such as pheochromocytoma or paraganglioma, are dangerous tumors that constitute a potential threat for a large number of patients. Currently, the biochemical diagnosis of neuroendocrine tumors is based on measurement of the direct secretory products of the adrenomedullary-sympathetic system or of their metabolites, such as catecholamines or their metanephrine derivatives, from plasma or urine. The techniques used for analysis of plasma free metanephrines, i.e. high-performance liquid chromatography or high-performance liquid chromatography coupled with mass-spectrometry are technically-demanding and time consuming, which limit their availability. Here we demonstrate a simple, fast and low-cost method for detecting metanephrine by Surface Enhanced Raman Scattering (SERS). The protocol consists in using evaporation-induced self-assembly of gold (Au) nanoparticles incubated with the analyte, on planar gold films. The assembly process produces regions with a dense distribution of both inter-particle gaps and particle-film gaps. Finite-difference time-domain simulations confirm that both kinds of gaps are locations of enhanced electromagnetic fields resulting from inter-particle and particle-film plasmonic coupling, useful for SERS amplification. Metanephrine vibrational bands assignment was performed according to density functional theory calculations. Metanephrine metabolite was detected in liquid at concentration levels lower than previously reported for other similar metabolites. The obtained results demonstrate that the Au nanoparticle/Au film exhibits noticeable SERS amplification of the adsorbed metabolite and can be used in the design of efficient, stable SERS-active substrates for the detection and identification of specific tumor markers.

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Abbreviations

HPLC:

High-performance liquid chromatography

SERS:

Surface Enhanced Raman Scattering

FDTD:

Finite-difference time-domain simulations

DFT:

Density functional theory

ELISA:

Enzyme-linked immunosorbent assay

AFM:

Atomic force microscopy

AuNPs:

Gold nanoparticles

TEM:

Transmission Electron Microscopy

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Acknowledgment

This work was supported by Babes-Bolyai University, Cluj-Napoca, Romania under the Research Grant for Young Scientists, Contract GTC-UBB No. 34056/2013. S. Boca acknowledges post-doctoral grant of the Romanian Ministry of Education, CNCS-UEFISCDI, Project number PN-II-RU-PD-2012-3-0111. M. Baia acknowledges COST action BM1401.

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Authors and Affiliations

  1. Nanobiophotonics and Laser Microspectroscopy Center, Interdisciplinary Research Institute on Bio-Nano-Sciences, Babes-Bolyai University, 42 Treboniu Laurian, 400271, Cluj-Napoca, Romania

    Sanda Boca, Cosmin Farcau, Monica Baia & Simion Astilean

  2. Biomolecular Physics Department, Faculty of Physics, Babes-Bolyai University, 1 M. Kogalniceanu, 400084, Cluj-Napoca, Romania

    Sanda Boca, Monica Baia & Simion Astilean

  3. Institute of Oncology Prof. Dr. Ion Chiricuta, 34-36 Republicii, 400015, Cluj-Napoca, Romania

    Sanda Boca

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  1. Sanda Boca
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  2. Cosmin Farcau
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  3. Monica Baia
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  4. Simion Astilean
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Correspondence to Sanda Boca or Simion Astilean.

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Electronic supplementary material

ESM 1

Nanoparticle size distribution by dynamic light scattering (DLS), extinction spectra of colloidal gold nanoparticles mixed with various concentrations of metanephrine, SERS spectra of metanephrine in aged colloid, optical images of circular deposit made by gold nanoparticles on gold film. (DOCX 1130 kb)

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Boca, S., Farcau, C., Baia, M. et al. Metanephrine neuroendocrine tumor marker detection by SERS using Au nanoparticle/Au film sandwich architecture. Biomed Microdevices 18, 12 (2016). https://doi.org/10.1007/s10544-016-0037-3

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  • DOI: https://doi.org/10.1007/s10544-016-0037-3

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