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Sub-population analysis of deformability distribution in heterogeneous red blood cell population

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Abstract

We present a method for sub-population analysis of deformability distribution using single-cell microchamber array (SiCMA) technology. It is a unique method allowing the correlation of overall cellular characteristics with surface and cytosolic characteristics to define the distribution of individual cellular characteristics in heterogeneous cell populations. As a proof of principle, reticulocytes, the immature sub-population of red blood cells (RBC), were recognized from RBC population by a surface marker and different characteristics on deformability between these populations were characterized. The proposed technology can be used in a variety of applications that would benefit from the ability to measure the distribution of cellular characteristics in complex populations, especially important to define hematologic disorders.

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Acknowledgments

This work was supported by the US National Institutes of Health grants 5R21HL092535-02, 1R24TW008822-01, and 1R43GM090515-01, and by the Converging Research Center Program funded by the Ministry of Education, Science and Technology (Project No. 2012K001442).

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Authors and Affiliations

  1. Central R&D Institute, Samsung Electro-mechanics Co., Ltd., Suwon, Republic of Korea

    Dong Woo Lee

  2. Center for Medical Metrology, Korea Research Institute of Standards and Science (KRISS), Daejeon, Republic of Korea

    Il Doh

  3. Department of Hematology, Children’s Hospital Oakland Research Institute, Oakland, CA, USA

    Frans A. Kuypers

  4. Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea

    Young-Ho Cho

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  1. Dong Woo Lee
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  2. Il Doh
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  3. Frans A. Kuypers
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  4. Young-Ho Cho
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Correspondence to Il Doh.

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Lee, D.W., Doh, I., Kuypers, F.A. et al. Sub-population analysis of deformability distribution in heterogeneous red blood cell population. Biomed Microdevices 17, 102 (2015). https://doi.org/10.1007/s10544-015-0007-1

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  • DOI: https://doi.org/10.1007/s10544-015-0007-1

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