Abstract
We present a method for sub-population analysis of deformability distribution using single-cell microchamber array (SiCMA) technology. It is a unique method allowing the correlation of overall cellular characteristics with surface and cytosolic characteristics to define the distribution of individual cellular characteristics in heterogeneous cell populations. As a proof of principle, reticulocytes, the immature sub-population of red blood cells (RBC), were recognized from RBC population by a surface marker and different characteristics on deformability between these populations were characterized. The proposed technology can be used in a variety of applications that would benefit from the ability to measure the distribution of cellular characteristics in complex populations, especially important to define hematologic disorders.
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Acknowledgments
This work was supported by the US National Institutes of Health grants 5R21HL092535-02, 1R24TW008822-01, and 1R43GM090515-01, and by the Converging Research Center Program funded by the Ministry of Education, Science and Technology (Project No. 2012K001442).
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Lee, D.W., Doh, I., Kuypers, F.A. et al. Sub-population analysis of deformability distribution in heterogeneous red blood cell population. Biomed Microdevices 17, 102 (2015). https://doi.org/10.1007/s10544-015-0007-1
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DOI: https://doi.org/10.1007/s10544-015-0007-1