Abstract
Traditional clinical methods for separating whole blood into blood cells and cell-free plasma are labor intensive and time consuming. Accordingly, the present study proposes a simple compact disk (CD) microfluidic platform for the rapid separation of plasma from whole human blood and the subsequent mixing of the plasma with a suitable reagent. The performance of three CD microfluidic platforms incorporating square-wave mixing channels with different widths is evaluated both numerically and experimentally. The results show that given an appropriate specification of the microchannel geometry and a CD rotation speed of 2000 rpm, a separation efficiency of 95 % can be achieved within 5 ~ 6 s given a diluted blood sample with a hematocrit concentration of 6 %. Moreover, a mixing efficiency of more than 96 % can be obtained within 5 s given a CD rotation speed of 2200 rpm. The practical feasibility of the proposed device is demonstrated by performing a prothrombin time (PT) test. It is shown that while the time required to perform the PT test using a conventional bench top system is around 15 min, the proposed CD microfluidic platform allows the test to be completed within 1 min.
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Acknowledgments
The authors gratefully acknowledge the financial support provided to this study by the National Science Council of Taiwan under Grant No. NSC 101-2221-E-150-036. In addition, the access provided to fabrication equipment by the Common Lab for Micro/Nano Science and Technology of National Formosa University is also greatly appreciated.
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Kuo, JN., Li, BS. Lab-on-CD microfluidic platform for rapid separation and mixing of plasma from whole blood. Biomed Microdevices 16, 549–558 (2014). https://doi.org/10.1007/s10544-014-9857-1
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DOI: https://doi.org/10.1007/s10544-014-9857-1