Abstract
In this work, antitumor compounds, lactoferrin [recombinant iron-free (Apo-rLf)], cisplatin (Cis) or their combination were embedded within a biodegradable polycaprolactone (PCL) polymer thin film, by a modified approach of a laser-based technique, matrix-assisted pulsed laser evaporation (MAPLE). The structural and morphological properties of the deposited hybrid films were analyzed by Fourier-transform infrared spectroscopy (FTIR) and atomic force microscopy (AFM). The in vitro effect on the cells’ morphology and proliferation of murine melanoma B16-F10 cells was investigated and correlated with the films’ surface chemistry and topography. Biological assays revealed decreased viability and proliferation, lower adherence, and morphological modifications in the case of melanoma cells cultured on both Apo-rLf and Cis thin films. The antitumor effect was enhanced by deposition of Apo-rLf with Cis within the same film. The unique capability of the new approach, based on MAPLE, to embed antitumor active factors within a biodegradable matrix for obtaining novel biodegradable hybrid platform with increased antitumor efficiency has been demonstrated.
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Acknowledgments
This work was supported by the Romanian Academy project 4/2012 of IBAR and a grant of the Romanian National Authority for Scientific Research, CNCS–UEFISCDI, project number PN-II-RU-TE-2011-3-0289. The financial support is gratefully acknowledged. We thank Ventria Bioscience for providing the recombinant lactoferrin for this study. Sima LE gratefully acknowledges the European Social Fund POSDRU 2007–2013 through the contract POSDRU/89/1.5/S/60746.
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Dinca, V., Florian, P.E., Sima, L.E. et al. MAPLE-based method to obtain biodegradable hybrid polymeric thin films with embedded antitumoral agents. Biomed Microdevices 16, 11–21 (2014). https://doi.org/10.1007/s10544-013-9801-9
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DOI: https://doi.org/10.1007/s10544-013-9801-9