Abstract
Surfactant nanocarriers have received considerable attention in the last several years as interesting alternative to classic liposomes. Different pH-sensitive vesicular colloidal carriers based on Tween 20 derivatives, obtained after functionalization of the head groups of the surfactant with natural, or simply modified, amino acids, were proposed as drug nanocarriers. Dynamic light scattering, Small Angle X-ray Scattering, Trasmission Electron Microscopy and fluorescence studies were used for the physico-chemical characterization of vesicles and mean size, size distribution, zeta potential, vesicle morphology and bilayer properties were evaluated. The pH-sensitivity and the stability of formulations, in absence and in presence of foetal bovine serum, were also evaluated. Moreover, the contact between surfactant vesicles and liposomes designed to model the cellular membrane was investigated by fluorescence studies to preliminary explore the potential interaction between vesicle and cell membranes. Experimental findings showed that physico-chemical and technological features of pH-sensitive vesicles were influenced by the composition of the carriers. Furthermore, proposed carriers are able to interact with mimetic cell membrane and it is reasonable to attribute the observed differences in interaction to the architectural/structural properties of Tween 20 derivatives. The findings reported in this investigation showed that a deep and extensive physico-chemical characterization of the carrier is a fundamental step, according to the evidence that the knowledge of nanocarrier properties is necessary to translate its potentiality to in vitro/in vivo applications.
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R. Abes, H.M. Moulton, P. Clair, S.T. Yang, S. Abes, K. Melikov, P. Prevot, D.S. Youngblood, P.L. Iversen, L.V. Chernomordik, B. Lebleu, Nucleic Acids Res. 36, 6343 (2008)
H. Amenitsch, M. Rappolt, M. Kriechbaum, H. Mio, P. Laggner, S. Bernstorff, J. Synchrotron. Radiat. 5, 506 (1998)
H. Amenitsch, G. Caracciolo, P. Foglia, V. Fuscoletti, P. Giansanti, C. Marianecci, D. Pozzi, A. Laganà, Colloids Surf. B-Biointerfaces 82, 141 (2011)
A.J. Baillie, A.T. Florence, L.R. Hume, G.T. Muirhead, A. Rogerson, J. Pharm. Pharmacol. 37, 863 (1985)
D. Bei, J. Meng, B.B. Youan, Nanomedicine 5, 1385 (2010)
M. Brecht, H. Degroot, Aminoacids 6, 25 (1994)
E. Canel, A. Gultepe, A. Dogan, E. Kiliç, J. Sol. Chem. 5, 5 (2006)
G. Caracciolo, G. Mancini, C. Bombelli, R. Caminiti, Chem. Phys. Lett. 386, 76 (2004)
M. Carafa, C. Marianecci, G. Lucania, E. Marchei, E. Santucci, J. Contr. Rel. 95, 67 (2004)
M. Carafa, L. Di Marzio, C. Marianecci, B. Cinque, G. Lucania, K. Kajiwara, M.G. Cifone, E. Santucci, Eur. J. Pharm. Sci. 28, 385 (2006)
E.J. Carvalho, M.C. dos Santos, ACS Nano. 4, 765 (2010)
J.A. Champion, Y.K. Katare, S. Mitragotri, J. Contr. Rel. 121, 3 (2007)
H.T. Clarke, H.B. Gillespie, S.Z. Weisshauss, J. Am. Chem. Soc. 55, 4571 (1933)
P. Couvreur, C. Vauthier, Pharm. Res. 23, 1417 (2006)
P. Decuzzi, B. Godin, T. Tanaka, S.Y. Lee, C. Chiappini, X. Liu, M. Ferrari, J. Contr. Rel. 141, 320 (2010)
L. Di Marzio, C. Marianecci, B. Cinque, M. Nazzarri, A.C. Cimini, L. Cristiano, M.G. Cifone, F. Alhaique, M. Carafa, Biochim. Biophys. Acta 1778, 2749 (2008)
L. Di Marzio, C. Marianecci, M. Petrone, F. Rinaldi, M. Carafa, Colloids Surf. B-Biointerfaces 82, 18 (2011)
D.E. Discher, A. Eisenberg, Science 297, 967 (2002)
V. Djinović, M. Momčilović, S. Grgurić-Šipka, V. Trajković, M. Mostarica Stojković, D. Miljković, T. Sabo, J. Inorg. Biochem. 98, 2168 (2004)
A. Dogan, N. Aslan, E. Canel, E. Kiliç, J. Sol. Chem. 39, 1589 (2010)
D.C. Drummond, M. Zignani, J.C. Leroux, Current status of pH-sensitive liposomes in drug delivery. Prog. Lip. Res. 39, 409 (2000)
E.J. Dufourc, E.J. Parish, S. Chitrakorn, I.C.P. Smith, Biochemistry 23, 6062 (1984)
A. El-Sayed, S. Futaki, H. Harashim, AAPS J. 11, 13 (2009)
W. Eschweiler, Ber. 38, 880 (1905)
O.C. Farokhzad, R. Langer, ACS Nano. 3, 16 (2009)
Y. Hao, F. Zhao, N. Li, Y. Yang, K. Li, Int. J. Pharm. 244, 73 (2002)
H.E. Hofland, R. van der Geest, H.E. Bodde, H.E. Junginger, J.A. Bouwstra, Pharm. Res. 11, 659 (1994)
M. Hossann, M. Wiggenhorn, A. Schwerdt, K. Wachholz, N. Teichert, H. Eibl, R.D. Issels, L.H. Lindner, Biochim. Biophys. Acta 1768, 2491 (2007)
G. Hungerford, E.M. Castanheira, A.L. Baptista, P.J. Coutinho, M.E. Oliveira, J. Fluoresc. 15, 835 (2005)
K. Ishihara, R. Tsujino, M. Hamada, N. Toyoda, Y. Iwasaki, Colloids Surf. B-Biointerfaces 25, 325 (2002)
M. Johnsson, A. Wagenaar, J.B.F.N. Engberts, J. Am. Chem. Soc. 125, 757 (2003)
K. Kano, J.H. Fendler, Biochim. Biophys. Acta 509, 289 (1978)
J.M. Lehn, Science 295, 2400 (2002)
B.R. Lentz, Chem. Phys. Lipids 50, 171 (1989)
A. Manconi, A.O. Vila, C. Sinico, J. Figueruelo, F. Molina, A.M. Fadda, J. Drug Deliv. Sci. Technol. 16, 141 (2006)
A. Manosroi, P. Wongtrakul, J. Manosroi, H. Sakai, F. Sugawara, M. Yuasa, M. Abe, Colloids Surf. B-Biointerfaces 30, 129 (2003)
C. Marianecci, D. Paolino, C. Celia, M. Fresta, M. Carafa, F. Alhaique, J. Contr. Rel. 147, 127 (2010)
A. Masotti, P. Vicennati, A. Alisi, C. Marianecci, F. Rinaldi, M. Carafa, G. Ortaggi, Bioorg. Med. Chem. Lett. 20, 3021 (2010)
F.M. Menger, K.D. Gabrielson, Angew Chem. Int. Ed. Engl. 34, 2091 (1995)
B. Mukherjee, B. Patra, B. Layek, A. Mukherjee, Int. J. Nanomedicine 2, 213 (2007)
R.H. Müller, C. Jacobs, O. Kayser, Adv. Drug Deliv. Rev. 47, 3 (2001)
R. Muzzalupo, L. Tavano, S. Trombino, R. Cassano, N. Picci, C. La Mesa, Colloid Surf. B-Biointerfaces 64, 200 (2008)
S. Nie, Y. Xing, G.J. Kim, J.W. Simons, Annu. Rev. Biomed. Eng. 9, 257 (2007)
T. Nyholm, J.P. Slotte, Langmuir 17, 4724 (2001)
G. Pabst, R. Koschuch, B. Pozo-Navas, M. Rappolt, K. Lohner, P. Laggner, J. Appl. Crystallogr. 36, 1378 (2003)
A. Polozova, F.M. Winnik, Langmuir 15, 4222 (1999)
A. Ribier, R.M. Handjani-Vila, E. Bardez, B. Valeur, Colloids Surf. 10, 155 (1984)
E.S. Rowe, F. Zhang, T.W. Leung, J.S. Parr, P.T. Guy, Biochemistry 37, 2430 (1998)
M. Shinitzky, Y. Barenholz, Biochim. Biophys. Acta 515, 367 (1978)
A.M. Smith, H. Duan, A.M. Mohs, S. Nie, Adv. Drug Deliv. Rev. 60, 1226 (2008)
M.C.A. Stuart, J.C. Van De Pas, J.B.F.N. Engberts, J. Phys. Org. Chem. 18, 929 (2005)
C. Terzano, L. Allegra, F. Alhaique, C. Marianecci, M. Carafa, Eur. J. Pharm. Biopharm. 59, 57 (2005)
I.F. Uchegbu, A.T. Florence, Adv. Colloid Interface Sci. 58, 1 (1995)
I.F. Uchegbu, S.P. Vyas, Int. J. Pharm. 172, 33 (1998)
D.A. Van Hal, J.A. Bouwstra, A. Van Rensen, E. Jeremiasse, T. De Vringer, H.E. Junginger, J. Colloid Interface Sci. 178, 263 (1996)
P. Ventakasen, Y. Cheng, D. Kahne, J. Am. Chem. Soc. 116, 6955 (1994)
M.R. Vist, J.H. Davis, Biochemistry 29, 451 (1990)
A.T. Yoshioka, B.B. Sternberg, A.T. Florence, Int. J. Pharm. 105, 1 (1994)
X. Zhao, Curr. Opin. Colloid Interface Sci. 14, 340 (2009)
Acknowledgements
The authors are grateful to Dr. Heinz Amenitsch, at the Institute of Biophysics and Nanosystems Research, Austrian Academy of Sciences (Graz—Austria), for his support in SAXS experiments, as well as for stimulating suggestions and discussion.
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Carlotta Marianecci and Federica Rinaldi equally contributed to the work.
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Marianecci, C., Rinaldi, F., Di Marzio, L. et al. Interaction of pH-sensitive non-phospholipid liposomes with cellular mimetic membranes. Biomed Microdevices 15, 299–309 (2013). https://doi.org/10.1007/s10544-012-9731-y
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DOI: https://doi.org/10.1007/s10544-012-9731-y