Article

Biomedical Microdevices

, Volume 15, Issue 4, pp 635-643

Proliferation and migration of tumor cells in tapered channels

  • Yuan WanAffiliated withNano-Bio Lab, University of Texas at ArlingtonDepartment of Bioengineering, University of Texas at ArlingtonNanotechnology Research and Education Center, University of Texas at ArlingtonMawson Institute, University of South Australia
  • , Deepika TamulyAffiliated withDepartment of Bioengineering, University of Texas at Arlington
  • , Peter B. AllenAffiliated withInstitute for Cell and Molecular Biology, University of Texas at Austin
  • , Young-tae KimAffiliated withDepartment of Bioengineering, University of Texas at ArlingtonNanotechnology Research and Education Center, University of Texas at Arlington
  • , Robert BachooAffiliated withAnnette G. Strauss Center for Neuro-Oncology, University of Texas Southwestern Medical CenterDepartment of Neurology, University of Texas Southwestern Medical CenterDepartment of Internal Medicine, University of Texas Southwestern Medical Center
  • , Andrew D. EllingtonAffiliated withInstitute for Cell and Molecular Biology, University of Texas at Austin
  • , Samir M. IqbalAffiliated withNano-Bio Lab, University of Texas at ArlingtonDepartment of Bioengineering, University of Texas at ArlingtonNanotechnology Research and Education Center, University of Texas at ArlingtonDepartment of Electrical Engineering, University of Texas at ArlingtonJoint Graduate Committee of Bioengineering Program, University of Texas at Arlington and University of Texas Southwestern Medical Center at Dallas, University of Texas at Arlington Email author 

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Abstract

Tumor cells depict two deviant tendencies; over-proliferation and vigorous migration. A tapered channel device is designed and fabricated for in vitro studies. We report inhibited proliferation and migration of human glioblastoma (hGBM) cells when exposed to an aptamer that is known to bind epidermal growth factor receptors (EGFR). The device is integrated with controlled ambient and microscope for providing real-time and quantitative characterization of the tumor cell behavior. The results show that hGBM cells loose proliferation and motility when exposed to the anti-EGFR aptamer. The aptamer directly inhibits and blocks EGF-induced EGFR phosphorylation. This also reduces the ability of cells to remodel their internal structure for invasion through narrow constrictions. This provides a framework for possible studies on efficacy of other inhibiting molecules.

Keywords

Inhibition of cancer Microfluidics Tapered channels Aggressive migration