Biomedical Microdevices

, Volume 13, Issue 3, pp 585–595

A suction-type microfluidic immunosensing chip for rapid detection of the dengue virus

Authors

  • Chen-Hsun Weng
    • Department of Microbiology and ImmunologyNational Cheng Kung University
  • Tze-Bin Huang
    • Department of Engineering ScienceNational Cheng Kung University
  • Chih-Chia Huang
    • Department of ChemistryNational Cheng Kung University
  • Chen-Sheng Yeh
    • Department of ChemistryNational Cheng Kung University
  • Huan-Yao Lei
    • Department of Microbiology and ImmunologyNational Cheng Kung University
    • Department of Power Mechanical EngineeringNational Tsing Hua University
Article

DOI: 10.1007/s10544-011-9529-3

Cite this article as:
Weng, C., Huang, T., Huang, C. et al. Biomed Microdevices (2011) 13: 585. doi:10.1007/s10544-011-9529-3

Abstract

The enzyme-linked immunosorbent assay (ELISA) is widely used in medical diagnostics. In order to reduce the diagnosis time and to lower the consumption of sample/reagents in an ELISA assay, a suction-type, automatic, pneumatically-driven microfluidic chip has been designed and fabricated in this study. The microfluidic chip integrates a multi-functional micro-transport/mixing unit, for transporting metering and mixing of samples and reagents in order to automatically perform the entire ELISA protocol. A new surface modification has been adopted which allows for a high processing capacity. The detection sensitivity for the dengue virus is found to be 101 PFU/ml, which is much better than a conventional ELISA assay (103 PFU/ml). The entire assay time is only 30 min, which is much faster than with 96-well microtiter plates (4 h). The consumed sample and reagent volume is only 12 μl, which is less than a conventional assay (100 μl). The development of this microfluidic chip may be promising for other immunosensing applications.

Keywords

MicrofluidicsSuctionImmunosensingELISADengue virusSurface modification

Nomenclature

BSA

Bovine serum albumin

CCD

Charge-coupled device

DI

Deionized

DV

Dengue virus

ELISA

Enzyme-linked immunosorbent assay

EMV

Electromagnetic valve

EV

Enterovirus

GA

Glutaraldehyde

HAU

Hemagglutination unit

HBV

Hepatitis B virus

HRP

Horse reddish peroxidase

IgG

Immunoglobulin G

IU

International unit

LBL

Layer-by-layer

OD

Optical density

PAA

Poly(acrylic acid)

PBS

Phosphate buffer saline

PDMS

Polydimethylsiloxane

PEI

Poly(ethyleneimine)

PEM

Polyelectrolyte multilayer

PFU

Plaque forming unit

Pluronic P123

Poly (ethylene oxide)-poly (propylene oxide)-poly (ethylene oxide) triblock copolymer

RT-PCR

Reverse-transcription polymerase chain reaction

TMB

Tetramethylbenzidin

UV

Ultraviolet

Copyright information

© Springer Science+Business Media, LLC 2011