Article

Biomedical Microdevices

, Volume 13, Issue 3, pp 585-595

A suction-type microfluidic immunosensing chip for rapid detection of the dengue virus

  • Chen-Hsun WengAffiliated withDepartment of Microbiology and Immunology, National Cheng Kung University
  • , Tze-Bin HuangAffiliated withDepartment of Engineering Science, National Cheng Kung University
  • , Chih-Chia HuangAffiliated withDepartment of Chemistry, National Cheng Kung University
  • , Chen-Sheng YehAffiliated withDepartment of Chemistry, National Cheng Kung University
  • , Huan-Yao LeiAffiliated withDepartment of Microbiology and Immunology, National Cheng Kung University
  • , Gwo-Bin LeeAffiliated withDepartment of Power Mechanical Engineering, National Tsing Hua University Email author 

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Abstract

The enzyme-linked immunosorbent assay (ELISA) is widely used in medical diagnostics. In order to reduce the diagnosis time and to lower the consumption of sample/reagents in an ELISA assay, a suction-type, automatic, pneumatically-driven microfluidic chip has been designed and fabricated in this study. The microfluidic chip integrates a multi-functional micro-transport/mixing unit, for transporting metering and mixing of samples and reagents in order to automatically perform the entire ELISA protocol. A new surface modification has been adopted which allows for a high processing capacity. The detection sensitivity for the dengue virus is found to be 101 PFU/ml, which is much better than a conventional ELISA assay (103 PFU/ml). The entire assay time is only 30 min, which is much faster than with 96-well microtiter plates (4 h). The consumed sample and reagent volume is only 12 μl, which is less than a conventional assay (100 μl). The development of this microfluidic chip may be promising for other immunosensing applications.

Keywords

Microfluidics Suction Immunosensing ELISA Dengue virus Surface modification