Biomedical Microdevices

, Volume 13, Issue 3, pp 565–571

Lectin-mediated microfluidic capture and release of leukemic lymphocytes from whole blood

Authors

  • Dwayne A. L. Vickers
    • Department of Chemical EngineeringNortheastern University
  • Marina Hincapie
    • Barnett Institute and Department of Chemistry & Chemical BiologyNortheastern University
  • William S. Hancock
    • Barnett Institute and Department of Chemistry & Chemical BiologyNortheastern University
    • Department of Chemical EngineeringNortheastern University
Article

DOI: 10.1007/s10544-011-9527-5

Cite this article as:
Vickers, D.A.L., Hincapie, M., Hancock, W.S. et al. Biomed Microdevices (2011) 13: 565. doi:10.1007/s10544-011-9527-5

Abstract

Lectins are a group of proteins that bind specifically and reversibly to mono- and oligosaccharide carbohydrate structures that are present on the surfaces of mammalian cells. The use of lectins as capture agents in microfluidic channels was examined with a focus on cells associated with T and B lymphocytic leukemia. In addition to examining the adhesion of Jurkat T and Raji B lymphocytes to a broad panel of lectins, this work also examined the capture of these cells from whole blood. Captured T and B lymphocytes were eluted from the microfluidic devices with a solution of the lectin’s inhibiting sugar. The capture and release steps were accomplished in under 1 h. The significance of this work lies within the realm of low-cost capture of abundant target cells with non-stimulatory elution capability.

Keywords

Microfluidics Lectins Cell capture Cell adhesion Blood Leukemia

Copyright information

© Springer Science+Business Media, LLC 2011