Biomedical Microdevices

, Volume 12, Issue 6, pp 1043–1049

An all-in-one microfluidic device for parallel DNA extraction and gene analysis

  • Yi Zhang
  • Seungkyung Park
  • Samuel Yang
  • Tza-Huei Wang
Article

DOI: 10.1007/s10544-010-9458-6

Cite this article as:
Zhang, Y., Park, S., Yang, S. et al. Biomed Microdevices (2010) 12: 1043. doi:10.1007/s10544-010-9458-6

Abstract

We have developed a microfluidic device capable of fully integrated sample preparation and gene analysis from crude biosamples such as whole blood. Our platform takes the advantage of the silica superparamagnetic particle based solid phase extraction to develop an all-in-one scheme that performs cell lysis, DNA binding, washing, elution and the PCR in the same reaction chamber. The device also employs a unique reagent loading scheme, allowing efficient preparation of multiple reactions via a single injection channel. In addition, PCR is performed in a droplet-in-oil manner, eliminating the need for chamber sealing. The combination of these design features greatly reduces the complexity in implementing fully integrated lab-on-a-chip systems for genetic detection, facilitating parallel analysis of multiple samples or genes on a single microchip. The capability of the device is demonstrated by performing DNA isolation from the human whole blood sample and analyzing the Rsf-1 gene using the TaqMan probe based gene specific PCR assays.

Keywords

Sample preparationSolid phase extractionAll-in-oneSilica superparamagnetic particlesPCRPDMS

Supplementary material

10544_2010_9458_MOESM1_ESM.doc (720 kb)
ESM 1(DOC 720 kb)

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Yi Zhang
    • 1
  • Seungkyung Park
    • 2
  • Samuel Yang
    • 2
  • Tza-Huei Wang
    • 1
    • 3
    • 4
  1. 1.Department of Biomedical Engineering, Johns Hopkins School of MedicineJohns Hopkins UniversityBaltimoreUSA
  2. 2.Department of Emergency MedicinesJohns Hopkins School of MedicineBaltimoreUSA
  3. 3.Department of Mechanical EngineeringJohns Hopkins UniversityBaltimoreUSA
  4. 4.The Sidney Kimmel Comprehensive Cancer CenterJohns Hopkins School of MedicineBaltimoreUSA