Article

Biomedical Microdevices

, Volume 12, Issue 6, pp 967-975

Handheld impedance biosensor system using engineered proteinaceous receptors

  • Ebrahim Ghafar-ZadehAffiliated withDepartment of Electrical and Computer Engineering, McGill University Email author 
  • , Shafinaz F. ChowdhuryAffiliated withLady Davis Institute for Medical Research, McGill University
  • , Amir AliakbarAffiliated withDepartment of Electrical and Computer Engineering, McGill University
  • , Vamsy ChodavarapuAffiliated withDepartment of Electrical and Computer Engineering, McGill University
  • , Rosemond LambroseAffiliated withLady Davis Institute for Medical Research, McGill University
  • , Lenord BeitalAffiliated withLady Davis Institute for Medical Research, McGill University
  • , Mohamad SawanAffiliated withDepartment of Electrical Engineering, Ecole Polytechnique de Montreal
  • , Mark TrifiroAffiliated withLady Davis Institute for Medical Research, McGill University

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Abstract

We put forward an impedometric protein-based biosensor platform suitable for point-of-care diagnostics. A hand-held scale impedance reader system is described for the detection of corresponding physiochemical changes as the immobilized proteins bind to the analyte molecules in the proximity of the microfabricated electrodes. Specifically, we study the viability of this approach for glucose biosensing purposes using genetically engineered glucokinase as receptor proteins. The proposed reagent-less biosensor offers a high sensitivity of 0.5 mM glucose concentration level in the physiologically relevant range of 0.5 mM to 7.5 mM with less than 10 s response time.

Keywords

Protein-based biosensor Impedometric readout system Microfabricated gold electrodes Glucokinase Glucose