Biomedical Microdevices

, Volume 12, Issue 6, pp 967–975

Handheld impedance biosensor system using engineered proteinaceous receptors

Authors

    • Department of Electrical and Computer EngineeringMcGill University
  • Shafinaz F. Chowdhury
    • Lady Davis Institute for Medical ResearchMcGill University
  • Amir Aliakbar
    • Department of Electrical and Computer EngineeringMcGill University
  • Vamsy Chodavarapu
    • Department of Electrical and Computer EngineeringMcGill University
  • Rosemond Lambrose
    • Lady Davis Institute for Medical ResearchMcGill University
  • Lenord Beital
    • Lady Davis Institute for Medical ResearchMcGill University
  • Mohamad Sawan
    • Department of Electrical EngineeringEcole Polytechnique de Montreal
  • Mark Trifiro
    • Lady Davis Institute for Medical ResearchMcGill University
Article

DOI: 10.1007/s10544-010-9451-0

Cite this article as:
Ghafar-Zadeh, E., Chowdhury, S.F., Aliakbar, A. et al. Biomed Microdevices (2010) 12: 967. doi:10.1007/s10544-010-9451-0

Abstract

We put forward an impedometric protein-based biosensor platform suitable for point-of-care diagnostics. A hand-held scale impedance reader system is described for the detection of corresponding physiochemical changes as the immobilized proteins bind to the analyte molecules in the proximity of the microfabricated electrodes. Specifically, we study the viability of this approach for glucose biosensing purposes using genetically engineered glucokinase as receptor proteins. The proposed reagent-less biosensor offers a high sensitivity of 0.5 mM glucose concentration level in the physiologically relevant range of 0.5 mM to 7.5 mM with less than 10 s response time.

Keywords

Protein-based biosensor Impedometric readout system Microfabricated gold electrodes Glucokinase Glucose

Copyright information

© Springer Science+Business Media, LLC 2010