Abstract
Cell-cell interaction is important in numerous biological processes, including cell growth, cell differentiation and migration. The ability to generate pre-determined cell patterns or cell arrays on a study surface is crucial for cell-cell interaction studies. In this paper, we developed a method for fast cell array fabrication using laser sintering and the hydrophobicity of PDMS films. This approach can be easily adopted and is cost-effective. Hydrophobic PDMS films were fabricated into polymeric chips containing hundreds of microwells. The films were then transferred onto tissue culture surfaces to separate cells in the formation of cell arrays (Patterned PDMS based cell array system, PCAS). We used NIH/3T3 fibroblast cells to demonstrate the feasibility of PCAS. The success of fast fabrication of patterned cell arrays was obtained using different initial cell seeding densities. We also used poorly adherent PC-12 cells to demonstrate the cell-cell communication. Results showed that the method is very useful for studying topics such as cell-cell interaction, cell-substrate interaction or cell migration.
Similar content being viewed by others
References
O.C. Attucks, K.S. Katula, FASEB J. 15, A258 (2001)
A. Bernard, J.P. Renault, B. Michel, H.R. Bosshard, E. Delamarche, Adv. Mater. 12, 1067 (2000)
S.N. Bhatia, U.J. Balis, M.L. Yarmush, M. Toner, Biotechnol. Prog. 14, 378 (1998)
C.J. Campbell, N. O’Looney, M. Chong Kwan, J.S. Robb, A.J. Ross, J.S. Beattie, J. Petrik, P. Ghazal, Anal. Chem. 78, 1930 (2006)
S. Fujita, Y. Morita, H. Iwata, Anal. Bioanal. Chem. 391, 2753 (2008)
J. Fukuda, A. Khademhosseini, J. Yeh, G. Eng, J.J. Cheng, O.C. Farokhzad, R. Langer, Biomaterials 27, 1479 (2006)
L.E. Heasley, Oncogene 20, 1563 (2001)
C.H. Hsieh, Y.Y. Huang, U.S. patent pending. Application number 12/468,814. (Date of filing May 19, 2009; Date of publication July 09, 2010)
S. Iwanaga, Y. Akiyama, A. Kikuchi, M. Yamato, K. Sakai, T. Okano, Biomaterials 26, 5395 (2005)
E.H. Javazon, D.C. Colter, E.J. Schwarz, D.J. Prockop, Stem Cells 19, 219 (2001)
R.S. Kane, S. Takayama, E. Ostuni, D.E. Ingber, G.M. Whitesides, Biomaterials 20, 2363 (1999)
K. Kuwahara, Y. Saito, O. Nakagawa, I. Kishimoto, M. Harada, E. Ogawa, Y. Miyamoto, I. Hamanaka, N. Kajiyama, N. Takahashi, T. Izumi, R. Kawakami, N. Tamura, Y. Ogawa, K. Nakao, FEBS Lett. 452, 314 (1999)
R. Langer, J.P. Vacanti, Science 260, 920 (1993)
J.N. Lee, X. Jiang, D. Ryan, G.M. Whitesides, Langmuir 20, 11684 (2004)
J.C. Lotters, W. Olthuis, P.H. Veltink, P. Bergveld, J. Micromech. Microeng. 7, 145 (1997)
J. Magdalena, T.H. Millard, L.M. Machesky, J. Cell Sci. 116, 743 (2003)
J.C. McDonald, D.C. Duffy, J.R. Anderson, D.T. Chiu, H. Wu, O.J. Schueller, G.M. Whitesides, Electrophoresis 21, 27 (2000)
H.C. Moeller, M.K. Mian, S. Shrivastava, B.G. Chung, A. Khademhosseini, Biomaterials 29, 752 (2008)
J. Nakanishi, Y. Kikuchi, S. Inoue, K. Yamaguchi, T. Takarada, M. Maeda, J. Am. Chem. Soc. 129, 6694 (2007)
R. Onuki-Nagasaki, A. Nagasaki, K. Hakamada, T.Q. Uyeda, S. Fujita, M. Miyake, J. Miyake, Lab Chip 8, 1502 (2008)
J.H. Park, K.D. Park, Y.H. Bae, Biomaterials 20, 943 (1999)
N. Patrito, C. McCague, P.R. Norton, N.O. Petersen, Langmuir 23, 715 (2007)
O. Quehenberger, J. Lipid Res. 46, 1582 (2005)
A. Roda, M. Guardigli, C. Russo, P. Pasini, M. Baraldini, Biotechniques 28, 492 (2000)
A. Rosenthal, A. Macdonald, J. Voldman, Biomaterials 28, 3208 (2007)
E.A. Roth, T. Xu, M. Das, C. Gregory, J.J. Hickman, T. Boland, Biomaterials 25, 3707 (2004)
R. Singhvi, A. Kumar, G.P. Lopez, G.N. Stephanopoulos, D.I. Wang, G.M. Whitesides, D.E. Ingber, Science 264, 696 (1994)
D.Y. Wang, Y.C. Huang, H.C. Chiang, A.M. Wo, Y.Y. Huang, J. Biomed. Mater. Res. B Appl. Biomater. 80B, 447 (2007)
K.A. Woodrow, M.J. Wood, J.K. Saucier-Sawyer, C. Solbrig, W.M. Saltzman, Tissue Eng. A 15, 1169 (2009)
Acknowledgement
Financial support from National Science Council of Taiwan, R.O.C. (NSC98-2221-E-002-045-MY3) is greatly acknowledged.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Hsieh, CH., Huang, CJ.C. & Huang, YY. Patterned PDMS based cell array system: a novel method for fast cell array fabrication. Biomed Microdevices 12, 897–905 (2010). https://doi.org/10.1007/s10544-010-9444-z
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10544-010-9444-z