Article

Biomedical Microdevices

, Volume 12, Issue 5, pp 887-896

An integrated microfluidic chip for non-immunological determination of urinary albumin

  • Chun-Che LinAffiliated withDepartment of Engineering Science, National Cheng Kung University
  • , Chin-Chung TsengAffiliated withDivision of Nephrology, Department of Internal Medicine, College of Medicine, National Cheng Kung University
  • , Chao-June HuangAffiliated withDepartment of Engineering Science, National Cheng Kung University
  • , Jung-Hao WangAffiliated withDepartment of Engineering Science, National Cheng Kung University
  • , Gwo-Bin LeeAffiliated withDepartment of Engineering Science, National Cheng Kung University Email author 

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Abstract

This study presents an integrated microfluidic chip for non-immunologically determining the concentrations of albumin in clinical urine samples. This microchip integrates membrane-type micromixers and a fan-shaped micropump capable of simultaneously and precisely delivering assay reagents to react with 6 urine samples in one single operation. The experimental results show that the coefficient of variation in the pumping rate is 2.42%. More importantly, using this unique chip design, only 2 electromagnetic valves are required for the actuation of the micromixer and the micropump. The working range of the proposed microchip is 2–200 mg/L of albumin, which covers the range of interest for the determination of microalbuminuria. Moreover, statistical analysis show that the results obtained by the proposed microchip are in good agreement with the conventional detection method, based on immunological assays. This simple, inexpensive and microchip-based platform presents a promising alternative to conventional immunological assays for measurement of urinary albumin, and is well suited for clinical applications.

Keywords

Microalbuminuria Immunoassay Microfluidics Micropump