Biomedical Microdevices

, Volume 12, Issue 5, pp 887–896

An integrated microfluidic chip for non-immunological determination of urinary albumin

  • Chun-Che Lin
  • Chin-Chung Tseng
  • Chao-June Huang
  • Jung-Hao Wang
  • Gwo-Bin Lee
Article

DOI: 10.1007/s10544-010-9443-0

Cite this article as:
Lin, C., Tseng, C., Huang, C. et al. Biomed Microdevices (2010) 12: 887. doi:10.1007/s10544-010-9443-0

Abstract

This study presents an integrated microfluidic chip for non-immunologically determining the concentrations of albumin in clinical urine samples. This microchip integrates membrane-type micromixers and a fan-shaped micropump capable of simultaneously and precisely delivering assay reagents to react with 6 urine samples in one single operation. The experimental results show that the coefficient of variation in the pumping rate is 2.42%. More importantly, using this unique chip design, only 2 electromagnetic valves are required for the actuation of the micromixer and the micropump. The working range of the proposed microchip is 2–200 mg/L of albumin, which covers the range of interest for the determination of microalbuminuria. Moreover, statistical analysis show that the results obtained by the proposed microchip are in good agreement with the conventional detection method, based on immunological assays. This simple, inexpensive and microchip-based platform presents a promising alternative to conventional immunological assays for measurement of urinary albumin, and is well suited for clinical applications.

Keywords

MicroalbuminuriaImmunoassayMicrofluidicsMicropump

Nomenclature

AB

albumin blue

AER

albumin excretion rate

BioFET

biological field-effect transistor

CVs

coefficients of variation

CNC

computer-numerically controlled

CI

confidence interval

DS

dextran sulfate

EMVs

electromagnetic valves

ELISA

enzyme-linked immunosorbent assay

HSA

human serum albumin

LOD

limit of detection

MAU

microalbuminuria

MOSFET

metal oxide semiconductor field effect transistor

MIP

molecularly imprinted polymer

NaOH

sodium hydroxide

NCVs

normally closed valves

PB

polybrene

PDMS

poly(dimethylsiloxane)

PMMA

polymethylmethacrylate

QCM

quartz crystal microbalance

SAM

self-assembled monolayer

SD

standard deviation

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Chun-Che Lin
    • 1
  • Chin-Chung Tseng
    • 2
  • Chao-June Huang
    • 1
  • Jung-Hao Wang
    • 1
  • Gwo-Bin Lee
    • 1
  1. 1.Department of Engineering ScienceNational Cheng Kung UniversityTainanTaiwan
  2. 2.Division of Nephrology, Department of Internal Medicine, College of MedicineNational Cheng Kung UniversityTainanTaiwan