Skip to main content
Log in

Size-based separation and collection of mouse pancreatic islets for functional analysis

  • Published:
Biomedical Microdevices Aims and scope Submit manuscript

Abstract

Islet size has recently been demonstrated to be an important factor in determining human islet transplantation outcomes. In this study, a multi-layered microfluidic device was developed and quantified for size-based separation of a heterogeneous population of mouse islets. The device was fabricated using standard soft lithography and polydimethylsiloxane (PDMS). Size-based separation was first demonstrated via injection of a heterogeneous population of glass beads between 50–300 µm in diameter which were separated into five sub-populations based on their diameter. Next, a heterogeneous population of mouse pancreatic islets, between 50–250 µm in diameter was separated into four sub-populations. Throughout this process the islets remained intact without any signs of damage, as indicated by cell viability staining. Islet glucose-stimulated insulin secretion of each sub-population of islets was also evaluated demonstrating that islets smaller than 150 µm have superior stimulation indexes (SI) compared to islets larger than 150 µm. In this study, we found that islets between 100 µm and 150 µm in diameter had the greatest SI value in a heterogeneous population of islets.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • A.A.S. Bhagat, S.S. Kuntaegowdanahalli, I. Papautsky, Lab Chip 8, 1906 (2008)

    Article  Google Scholar 

  • S. Choi, J.K. Park, Lab Chip 7, 890 (2007)

    Article  Google Scholar 

  • C.B. Fuh, Anal. Chem. 72, 266A (2000)

    Google Scholar 

  • B.S. Hardy, K. Uechi, J. Zhen, H. Pirouz Kavehpour, Lab Chip 9, 935 (2008)

    Article  Google Scholar 

  • L.R. Huang, E.C. Cox, R.H. Austin, J.C. Sturm, Science 304, 987 (2004)

    Article  Google Scholar 

  • D. Huh, J.H. Bahng, Y. Ling, H.-H. Wei, O.D. Kripfgans, J.B. Fowlkes, J.B. Grotberg, S. Takayama, Anal. Chem. 79, 1369 (2007)

    Article  Google Scholar 

  • J. Jo, H. Kang, M.Y. Choi, D.S. Koh, Biophys. J. 89, 1534 (2005)

    Article  Google Scholar 

  • T. Kulrattanarak, R.G.M. van der Sman, C.G.P.H. Schroën, R.M. Boom, Adv. Colloid Interface Sci. 142, 53 (2008)

    Article  Google Scholar 

  • E.P. Lacy, M. Kostianovky, Diabetes 16, 35 (1967)

    Google Scholar 

  • E. Leclere, Y. Sakai, T. Fujii, 2003 IEEE, 415 (2003)

  • R. Lehmann, R.A. Zuellig, P. Kugelmeier, P.B. Baenninger, W. Moritz, A. Perren, P.A. Clavien, M. Weber, G.A. Spinas, Diabetes 56, 594 (2007)

    Article  Google Scholar 

  • P. Lillehoj, N. Li, H. Tsutsui, and C-M. Ho, 2008 IEEE, 292 (2008)

  • R.R. MacGregor, S.J. Williams, P.Y. Tong, K. Kover, W.V. Moore, L. Stehno-Bittel, Am J Physiol Endocrinol Metab. 290, 771 (2006)

    Article  Google Scholar 

  • K. Nam, D.T. Eddington, Size based separation of microparticles in a multilayered microfluidic device, J. Microelectromechanical Systems 19(2), 375–383 (2010)

    Google Scholar 

  • K.K. Papas, R.C. Long Jr., I. Constantinidis, A. Sambanis, Biochim. Biophys. Acta. 1291, 163 (1996)

    Google Scholar 

  • F.R. Phelan Jr., B.J. Bauer, Chem Eng Sci 64, 1747 (2009)

    Article  Google Scholar 

  • Y. Sai, M. Yamada, M. Yasuda, M. Seki, J. Chrom. A 1127, 214 (2006)

    Article  Google Scholar 

  • P. Salehi, M.A. Hansen, J.G. Avila, B. Barbaro, A. Gangemi, T. Romagnoli, Y. Wang, M. Qi, P. Murdock, E. Benedetti, J. Oberholzer, Transplantation 82, 983 (2006)

    Article  Google Scholar 

  • J. Takagi, M. Yamada, M. Yasuda, M. Seki, Lab Chip 5, 778 (2005)

    Article  Google Scholar 

  • H. Tsutsui, C.-M. Ho, Mech Res Comm 36, 92 (2009)

    Article  Google Scholar 

  • M.A. Unger, H.-P. Chou, T. Thorsen, A. Scherer, S.R. Quake, Science 288, 113 (2000)

    Article  Google Scholar 

  • M.D. Vahey, J. Voldman, Anal. Chem. 80, 3135 (2008)

    Article  Google Scholar 

  • X.B. Wang, J. Vykoukal, F.F. Becker, P.R. Gascoyne, Biophys. J. 74, 2689 (1998)

    Article  Google Scholar 

  • Y. Xia, G.M. Whitesides, Annu. Rev. Mater. Sci. 28, 153 (1998)

    Article  Google Scholar 

  • M. Yamada, M. Nakashima, M. Seki, Anal. Chem. 76, 5465 (2004)

    Article  Google Scholar 

  • M. Yamada, K. Kano, Y. Tsuda, J. Kobayashi, M. Yamato, M. Seki, T. Okano, Biomed. Microdevices 9, 637 (2007)

    Article  Google Scholar 

  • J. Yang, Y. Huang, X.-B. Wang, F.F. Becker, P.R.C. Gascoyne, Anal. Chem. 71, 911 (1999)

    Article  Google Scholar 

  • S. Yang, A. Undar, J.D. Zahn, Lab Chip 6, 871 (2006)

    Article  Google Scholar 

  • Y. Zhang, R.W. Barber, D.R. Emerson, Curr. Anal. Chem. 1, 345 (2005)

    Article  MATH  Google Scholar 

Download references

Acknowledgement

This work was supported by the NIH (JO) and the Alfred P. Sloan Foundation (DTE).

Author information

Authors and Affiliations

Authors

Corresponding authors

Correspondence to Jose Oberholzer or David T. Eddington.

Additional information

Equal contribution: Ki-Hwan Nam and Wang Yong.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nam, KH., Yong, W., Harvat, T. et al. Size-based separation and collection of mouse pancreatic islets for functional analysis. Biomed Microdevices 12, 865–874 (2010). https://doi.org/10.1007/s10544-010-9441-2

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10544-010-9441-2

Keywords

Navigation