Article

Biomedical Microdevices

, Volume 12, Issue 5, pp 809-819

Adhesion and proliferation of skeletal muscle cells on single layer poly(lactic acid) ultra-thin films

  • Leonardo RicottiAffiliated withScuola Superiore Sant’AnnaCRIM & ARTS Lab—Scuola Superiore Sant’Anna Email author 
  • , Silvia TaccolaAffiliated withScuola Superiore Sant’Anna
  • , Virginia PensabeneAffiliated withItalian Institute of Technology (IIT)
  • , Virgilio MattoliAffiliated withItalian Institute of Technology (IIT)
  • , Toshinori FujieAffiliated withFaculty of Science and Engineering, Waseda Universtiy (TWIns)
  • , Shinji TakeokaAffiliated withFaculty of Science and Engineering, Waseda Universtiy (TWIns)
  • , Arianna MenciassiAffiliated withScuola Superiore Sant’AnnaItalian Institute of Technology (IIT)
  • , Paolo DarioAffiliated withScuola Superiore Sant’AnnaItalian Institute of Technology (IIT)

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Abstract

An increasing interest in bio-hybrid systems and cell-material interactions is evident in the last years. This leads towards the development of new nano-structured devices and the assessment of their biocompatibility. In the present study, the development of free-standing single layer poly(lactic acid) (PLA) ultra-thin films is described, together with the analysis of topography and roughness properties. The biocompatibility of the PLA films has been tested in vitro, by seeding C2C12 skeletal muscle cells, and thus assessing cells shape, density and viability after 24, 48 and 72 h. The results show that free-standing flexible PLA nanofilms represent a good matrix for C2C12 cells adhesion, spreading and proliferation. Early differentiation into myotubes is also allowed. The biocompatibility of the novel ultra-thin films as substrates for cell growth promotes their application in the fields of regenerative medicine, muscle tissue engineering, drug delivery, and—in general—in the field of bio-hybrid devices.

Keywords

Nanofilms Biocompatibility Poly(lactic acid) Skeletal muscle cells C2C12 Bio-hybrid devices