Biomedical Microdevices

, Volume 12, Issue 3, pp 555–568

Hydrogel cell patterning incorporating photocaged RGDS peptides

Authors

  • Catherine A. Goubko
    • Department of Chemical and Biological EngineeringUniversity of Ottawa
  • Swapan Majumdar
    • Ottawa Health Research Institute, Loeb Building, Chronic Diseases ProgramThe Ottawa Hospital
  • Ajoy Basak
    • Ottawa Health Research Institute, Loeb Building, Chronic Diseases ProgramThe Ottawa Hospital
    • Department of Chemical and Biological EngineeringUniversity of Ottawa
    • Ottawa-Carleton Institute for Biomedical EngineeringUniversity of Ottawa
Article

DOI: 10.1007/s10544-010-9412-7

Cite this article as:
Goubko, C.A., Majumdar, S., Basak, A. et al. Biomed Microdevices (2010) 12: 555. doi:10.1007/s10544-010-9412-7

Abstract

As we aim towards enhancing our knowledge of complex cell behaviors and developing intricate cell-based devices and improved therapeutics, it becomes imperative that we be able to control and manipulate the spatial localization of cells. Here we have developed a novel strategy to pattern cells using a hyaluronic acid hydrogel material and photocaged RGDS (Arg-Gly-Asp-Ser) peptides. In this report, we discuss the chemical synthesis and photoactive properties of the caged peptides as well as the subsequent binding of these peptides to our hydrogel base. We further demonstrate the ability of this modified hydrogel material to pattern fibroblast cells on the micron scale using near-UV light exposure through a patterned photomask to selectively switch areas of the hydrogel surface from cell non-adhesive to cell adhesive. The cells are found to adhere and proliferate along the developed line patterns for at least 2.5 days, demonstrating significantly enhanced pattern longevity in comparison with previously reported studies.

Keywords

Cell adhesion Extracellular matrix Micropatterning Hyaluronic acid Peptide

Supplementary material

10544_2010_9412_MOESM1_ESM.doc (10.4 mb)
Supplemental Fig. 1S 1H NMR spectrum of caged-R[G]DS tetrapeptide in D2O with expanded portions within the insets (DOC 10628 kb)
10544_2010_9412_MOESM2_ESM.doc (10.4 mb)
Supplemental Fig. 2S 1H NMR spectrum of caged R[G]DS tetrapeptide in D2O following UV-irradiation with expanded portions within the insets (DOC 10663 kb)

Copyright information

© Springer Science+Business Media, LLC 2010