Article

Biomedical Microdevices

, Volume 12, Issue 3, pp 555-568

Hydrogel cell patterning incorporating photocaged RGDS peptides

  • Catherine A. GoubkoAffiliated withDepartment of Chemical and Biological Engineering, University of Ottawa
  • , Swapan MajumdarAffiliated withOttawa Health Research Institute, Loeb Building, Chronic Diseases Program, The Ottawa Hospital
  • , Ajoy BasakAffiliated withOttawa Health Research Institute, Loeb Building, Chronic Diseases Program, The Ottawa Hospital
  • , Xudong CaoAffiliated withDepartment of Chemical and Biological Engineering, University of OttawaOttawa-Carleton Institute for Biomedical Engineering, University of Ottawa Email author 

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Abstract

As we aim towards enhancing our knowledge of complex cell behaviors and developing intricate cell-based devices and improved therapeutics, it becomes imperative that we be able to control and manipulate the spatial localization of cells. Here we have developed a novel strategy to pattern cells using a hyaluronic acid hydrogel material and photocaged RGDS (Arg-Gly-Asp-Ser) peptides. In this report, we discuss the chemical synthesis and photoactive properties of the caged peptides as well as the subsequent binding of these peptides to our hydrogel base. We further demonstrate the ability of this modified hydrogel material to pattern fibroblast cells on the micron scale using near-UV light exposure through a patterned photomask to selectively switch areas of the hydrogel surface from cell non-adhesive to cell adhesive. The cells are found to adhere and proliferate along the developed line patterns for at least 2.5 days, demonstrating significantly enhanced pattern longevity in comparison with previously reported studies.

Keywords

Cell adhesion Extracellular matrix Micropatterning Hyaluronic acid Peptide