Biomedical Microdevices

, Volume 12, Issue 3, pp 505–511

A micro-channel-well system for culture and differentiation of embryonic stem cells on different types of substrate

  • Li Liu
  • Chunxiong Luo
  • Xiaofeng Ni
  • Li Wang
  • Kaori Yamauchi
  • Shinichi M. Nomura
  • Norio Nakatsuji
  • Yong Chen
Article

DOI: 10.1007/s10544-010-9407-4

Cite this article as:
Liu, L., Luo, C., Ni, X. et al. Biomed Microdevices (2010) 12: 505. doi:10.1007/s10544-010-9407-4

Abstract

We have developed a combined micro-channel and micro-well system for easy cell loading, culture and post-culture operation on a chip. To demonstrate the reliability of the system, on chip cell culture and differentiation were performed with different types of substrates made of culture dish, glass cover slide and polydimethylsiloaxe (PDMS). As expected, mouse embryo fibroblasts (MEF) showed different adhesion and growth rate on different substrates. When embryonic stem (ES) cells were co-cultured with MEFs, the formation of ES colonies is efficient on both glass and Petri dish, although PDMS could also be used. Finally, ES cell differentiation with neuron growth factors was performed on different substrates, showing clear advantages of using culture Petri dish over both glass and PDMS.

Keywords

MicrofluidicsEmbryonic stem cellsCell cultureCell differentiationSubstrate

Abbreviations

ES

embryonic stem

EB

embryo bodies

mES

mouse embryonic stem

MEF

mouse embryo fibroblasts

LIF

leukemia inhibitory factor

PS

polystyrene

RA

retinoic acid

DAPI

4′-6-Diamidino-2-phenylindole

DMEM

Dulbecco’s Modified Eagle’s Medium

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Li Liu
    • 1
  • Chunxiong Luo
    • 1
    • 2
  • Xiaofeng Ni
    • 1
    • 3
  • Li Wang
    • 1
    • 3
  • Kaori Yamauchi
    • 1
  • Shinichi M. Nomura
    • 1
    • 4
  • Norio Nakatsuji
    • 1
  • Yong Chen
    • 1
    • 3
  1. 1.Institute for Integrated Cell-Material Sciences (iCeMS)Kyoto UniversityKyotoJapan
  2. 2.Center for Microfluidic and NanotechnologyPeking UniversityBeijingChina
  3. 3.Ecole Normale SupérieureCNRS-ENS-UPMC UMR 8640ParisFrance
  4. 4.PRESTOJapan Science and Technology Agency (JST)TokyoJapan