Article

Biomedical Microdevices

, Volume 12, Issue 3, pp 505-511

A micro-channel-well system for culture and differentiation of embryonic stem cells on different types of substrate

  • Li LiuAffiliated withInstitute for Integrated Cell-Material Sciences (iCeMS), Kyoto University
  • , Chunxiong LuoAffiliated withInstitute for Integrated Cell-Material Sciences (iCeMS), Kyoto UniversityCenter for Microfluidic and Nanotechnology, Peking University
  • , Xiaofeng NiAffiliated withInstitute for Integrated Cell-Material Sciences (iCeMS), Kyoto UniversityEcole Normale Supérieure, CNRS-ENS-UPMC UMR 8640
  • , Li WangAffiliated withInstitute for Integrated Cell-Material Sciences (iCeMS), Kyoto UniversityEcole Normale Supérieure, CNRS-ENS-UPMC UMR 8640
  • , Kaori YamauchiAffiliated withInstitute for Integrated Cell-Material Sciences (iCeMS), Kyoto University
  • , Shinichi M. NomuraAffiliated withInstitute for Integrated Cell-Material Sciences (iCeMS), Kyoto UniversityPRESTO, Japan Science and Technology Agency (JST)
  • , Norio NakatsujiAffiliated withInstitute for Integrated Cell-Material Sciences (iCeMS), Kyoto University
  • , Yong ChenAffiliated withInstitute for Integrated Cell-Material Sciences (iCeMS), Kyoto UniversityEcole Normale Supérieure, CNRS-ENS-UPMC UMR 8640 Email author 

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Abstract

We have developed a combined micro-channel and micro-well system for easy cell loading, culture and post-culture operation on a chip. To demonstrate the reliability of the system, on chip cell culture and differentiation were performed with different types of substrates made of culture dish, glass cover slide and polydimethylsiloaxe (PDMS). As expected, mouse embryo fibroblasts (MEF) showed different adhesion and growth rate on different substrates. When embryonic stem (ES) cells were co-cultured with MEFs, the formation of ES colonies is efficient on both glass and Petri dish, although PDMS could also be used. Finally, ES cell differentiation with neuron growth factors was performed on different substrates, showing clear advantages of using culture Petri dish over both glass and PDMS.

Keywords

Microfluidics Embryonic stem cells Cell culture Cell differentiation Substrate