Biomedical Microdevices

, Volume 12, Issue 3, pp 499–503

An automatic microturbidostat for bacterial culture at constant density

Article

DOI: 10.1007/s10544-010-9406-5

Cite this article as:
Luo, X., Shen, K., Luo, C. et al. Biomed Microdevices (2010) 12: 499. doi:10.1007/s10544-010-9406-5

Abstract

We have developed a microturbidostat for long time bacterial culture at constant density controlled by optical detection and integrated pneumatic valves. The device was fabricated by multilayer soft lithography and in-situ formation of an agarose filter. The culture chamber of bacteria was connected in one side to a single bacterial input-output channel and in another side to a nutrient channel in which the agarose filter was formed to ensure the diffusion of nutrients and metabolites without bacterial loss. The bacterial number in the culture chamber was determined by measuring the fluorescence intensity of GFP proteins of the bacteria and the redundant bacteria could be exported automatically through the input-output channel with integrated micro-valves. In order to optimize the operation performance, we investigated the bacterial exportation efficiency with different input-output channel widths. As expected, the bacterial sorting coefficient was proportional to the input-output channel width. The results also showed that with a 20 µm channel-width, a long time culture was possible with a constant bacterial number in the chamber in the range from 400 to 700.

Keywords

MicrofluidicsMicroturbidostatBacterial growth

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Center for Microfluidics and Nanotechnology and School of Physics, The State Key Laboratory for Artificial Microstructures and Mesoscopic PhysicsPeking UniversityBeijingChina
  2. 2.Ecole Normale SuperieureCNRS-ENS-UPMC UMR 8640ParisFrance
  3. 3.Center for Theoretical Biology, Academy for Advanced Interdisciplinary StudiesPeking UniversityBeijingChina