Biomedical Microdevices

, Volume 12, Issue 3, pp 485-497

First online:

Fast and continuous plasma extraction from whole human blood based on expanding cell-free layer devices

  • Elodie SollierAffiliated withDepartment of Technology for Biology and Health, CEA-LETI-Minatec Email author 
  • , Myriam CubizollesAffiliated withDepartment of Technology for Biology and Health, CEA-LETI-Minatec
  • , Yves FouilletAffiliated withDepartment of Technology for Biology and Health, CEA-LETI-Minatec
  • , Jean-Luc AchardAffiliated withLEGI, Laboratoire des Ecoulements Géophysiques et Industriels, Domaine Universitaire

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This paper presents promising microfluidic devices designed for continuous and passive extraction of plasma from whole human blood. These designs are based on red cells lateral migration and the resulting cell-free layer locally expanded by geometric singularities such as an enlargement of the channel or a cavity adjacent to the channel. After an explanation of flow patterns, different tests are described that confirm the advantages of both proposed singularities, providing a 1.5 and 2X increase in extraction yield compared to a reference device, for 1:20 diluted blood at 100 µL/min. Devices have also been successively optimized, with extraction yields up to 17.8%, and biologically validated for plasma extraction, with no protein loss or denaturation, no hemolysis and with excellent cell purity. Finally, the dilution effect has been experimentally investigated.


Plasma extraction Cell-free layer Singularities Recirculation flows Blood