Biomedical Microdevices

, Volume 12, Issue 3, pp 435–442

Development of a protein sensing device utilizing interactions between polyaniline and a polymer acid dopant

  • Carolyn L. Bayer
  • Alper A. Konuk
  • Nicholas A. Peppas
Article

DOI: 10.1007/s10544-010-9400-y

Cite this article as:
Bayer, C.L., Konuk, A.A. & Peppas, N.A. Biomed Microdevices (2010) 12: 435. doi:10.1007/s10544-010-9400-y

Abstract

Human disease processes are often characterized by a deviation from the normal physiological concentration of critical biomarkers. The detection of disease biomarkers requires the development of novel sensing methods which are sensitive, specific, efficient and low-cost. To address this need, the ability of a device, which incorporates a film of polymer acid doped polyaniline, to respond to proteins at physiological pH and ionic strength was assessed. The conductive polymer was found to respond by changing conductivity in the presence of biomolecules, demonstrating a direct chemical to electronic transduction method. In future work, specificity can be incorporated into the system by integrating the conductive polymer with a protein selective film. The demonstration of a conductive polymer which is responsive to proteins at physiological conditions is a step towards the integration of these materials into implantable sensing systems.

Keywords

Conductive polymersPolyanilineBiosensorBovine serum albuminLysozyme

Abbreviations

PANI

polyaniline

PAAMPSA

poly(2-acrylamido-2-methylpropanesulfonic acid)

Lys

lysozyme

BSA

bovine serum albumin

APTS

aminopropyltrimethoxysilane

APS

ammonium persulfate

PBS

phosphate buffered solution

NaCl

sodium chloride

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Carolyn L. Bayer
    • 1
  • Alper A. Konuk
    • 2
  • Nicholas A. Peppas
    • 1
    • 2
    • 3
  1. 1.Department of Biomedical Engineering, Center on Biomaterials, Drug Delivery, Bionanotechnology and Molecular RecognitionThe University of Texas at AustinAustinUSA
  2. 2.Department of Chemical Engineering, Center on Biomaterials, Drug Delivery, Bionanotechnology and Molecular RecognitionThe University of Texas at AustinAustinUSA
  3. 3.Department of Pharmaceutics, Center on Biomaterials, Drug Delivery, Bionanotechnology and Molecular RecognitionThe University of Texas at AustinAustinUSA