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An implantable Teflon chip holding lithium naphthalocyanine microcrystals for secure, safe, and repeated measurements of pO2 in tissues

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Abstract

Lithium naphthalocyanine (LiNc) is a crystalline material that has significant potential as a probe for EPR (electron paramagnetic resonance)-based biological oximetry (Pandian et al. J. Mater. Chem. 19:4138–4147, 2009a). However, implantation of LiNc crystals in tissues in raw or neat form is undesirable since dispersion of crystals in tissue may lead to loss of EPR signal, while also exacerbating biocompatibility concerns due to tissue exposure. To overcome these concerns, we have encapsulated LiNc crystals in an oxygen-permeable polymer, Teflon AF 2400 (TAF). Fabrication of TAF films incorporating LiNc particles (denoted as LiNc:TAF chip) was carried out using solvent-evaporation techniques. The EPR linewidth of LiNc:TAF chip was linearly dependent on oxygen-partial pressure (pO2) and did not change significantly relative to neat LiNc crystals. LiNc:TAF chip responded to changes in pO2 reproducibly, enabling dynamic measurements of oxygenation in real time. The LiNc:TAF chips were stable in tissues for more than 2 months and were capable of providing repeated measurements of tissue oxygenation for extended periods of time. The results demonstrated that the newly fabricated, highly oxygen-sensitive LiNc:TAF chip will enhance the applicability of EPR oximetry for long-term and clinical applications.

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References

  • A.Y. Alentiev, Y.P. Yampolskii, V.P. Shantarovich, S.M. Nemser, N.A. Plate, J. Membr. Sci. 126, 123–132 (1997)

    Article  Google Scholar 

  • A. Bratasz, R.P. Pandian, G. Ilangovan, P. Kuppusamy, Adv. Exp. Med. Biol. 578, 375–380 (2006)

    Article  Google Scholar 

  • M. Dinguizli, S. Jeumont, N. Beghein, J. He, T. Walczak, P.N. Lesniewski, H. Hou, O.Y. Grinberg, A. Sucheta, H.M. Swartz, B. Gallez, Biosens. Bioelectron. 21(7), 1015–1022 (2006)

    Article  Google Scholar 

  • M. Dinguizli, N. Beghein, B. Gallez, Physiol. Meas. 29(11), 1247–1254 (2008)

    Article  Google Scholar 

  • E. Eteshola, R.P. Pandian, S.C. Lee, P. Kuppusamy, Biomed. Microdevices 11(2), 379–387 (2009)

    Article  Google Scholar 

  • G. Ilangovan, A. Bratasz, H. Li, P. Schmalbrock, J.L. Zweier, P. Kuppusamy, Magn. Reson. Med. 52(3), 650–657 (2004)

    Article  Google Scholar 

  • A.C. Kulkarni, P. Kuppusamy, N. Parinandi, Antioxid. Redox Signal. 9(10), 1717–1730 (2007)

    Article  Google Scholar 

  • V.K. Kutala, N.L. Parinandi, R.P. Pandian, P. Kuppusamy, Antioxid. Redox Signal. 6(3), 597–603 (2004)

    Article  Google Scholar 

  • K.J. Liu, P. Gast, M. Moussavi, S.W. Norby, N. Vahidi, T. Walczak, M. Wu, H.M. Swartz, Proc. Natl Acad. Sci. USA 90(12), 5438–5442 (1993)

    Article  Google Scholar 

  • G. Meenakshisundaram, E. Eteshola, R.P. Pandian, A. Bratasz, S.C. Lee, P., Kuppusamy. Biomed. Microdevices 11(4), 773–782 (2009a)

  • G. Meenakshisundaram, E. Eteshola, R.P. Pandian, A. Bratasz, K. Selvendiran, S.C. Lee, M.C. Krishna, H.M. Swartz, P. Kuppusamy, Biomed. Microdevices 11(4), 817–826 (2009b)

  • T.C. Merkel, I. Pinnav, R.S. Prabhakar, B.D. Freeman, Gas and vapor transport properties of perfluropolymers, in Materials science of membranes for gas and vapor separation, ed. by Y. Yampolskii, I. Pinnav, B.D. Freeman (Wiley, Chichester, 2006)

    Google Scholar 

  • R.P. Pandian, N.L. Parinandi, G. Ilangovan, J.L. Zweier, P. Kuppusamy, Free Radic. Biol. Med. 35(9), 1138–1148 (2003)

    Article  Google Scholar 

  • R.P. Pandian, Y. Kim, P.M. Woodward, J.M. Zweier, P.T. Manoharan, P. Kuppusamy, J. Mater. Chem. 16(36), 3609–3618 (2006)

    Article  Google Scholar 

  • R.P. Pandian, M. Dolgos, V. Dang, J.Z. Sostaric, P.M. Woodward, P. Kuppusamy, Chem. Mater. 19(14), 3545–3552 (2007)

    Article  Google Scholar 

  • R.P. Pandian, M. Dolgos, C. Marginean, P.M. Woodward, P. Chris Hammel, P.T. Manoharan, P. Kuppusamy, J. Mater. Chem. 19, 4138–4147 (2009a)

    Article  Google Scholar 

  • R.P. Pandian, S.M. Chacko, M.L. Kuppusamy, B.K. Rivera, P. Kuppusamy, Adv. Exp. Med. Biol. (In press) (2009b)

  • R. Springett, H.M. Swartz, Antioxid. Redox Signal. 9(8), 1295–1301 (2007)

    Article  Google Scholar 

  • H.M. Swartz, N. Khan, Biol Magn Reson 23, 197–228 (2005)

    Article  Google Scholar 

Download references

Acknowledgements

The study was supported by NIH grant EB004031. We would like to thank Dr. Gunjan Agarwal at the AFM Core Lab, The Ohio State University Medical Center for AFM analysis.

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Correspondence to Periannan Kuppusamy.

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Pandian, R.P., Meenakshisundaram, G., Bratasz, A. et al. An implantable Teflon chip holding lithium naphthalocyanine microcrystals for secure, safe, and repeated measurements of pO2 in tissues. Biomed Microdevices 12, 381–387 (2010). https://doi.org/10.1007/s10544-009-9394-5

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  • DOI: https://doi.org/10.1007/s10544-009-9394-5

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