Biomedical Microdevices

, Volume 12, Issue 3, pp 371–379

Internalization and cytotoxicity analysis of silicon-based microparticles in macrophages and embryos

Authors

  • Elisabet Fernández-Rosas
    • Departament Biologia Cel·lular, Fisiologia i ImmunologiaUniversitat Autònoma de Barcelona
    • Instituto de Microelectrónica de Barcelona (CNM-CSIC)
  • Rodrigo Gómez
    • Instituto de Microelectrónica de Barcelona (CNM-CSIC)
  • Elena Ibañez
    • Departament Biologia Cel·lular, Fisiologia i ImmunologiaUniversitat Autònoma de Barcelona
  • Lleonard Barrios
    • Departament Biologia Cel·lular, Fisiologia i ImmunologiaUniversitat Autònoma de Barcelona
  • Marta Duch
    • Instituto de Microelectrónica de Barcelona (CNM-CSIC)
  • Jaume Esteve
    • Instituto de Microelectrónica de Barcelona (CNM-CSIC)
  • José A. Plaza
    • Instituto de Microelectrónica de Barcelona (CNM-CSIC)
    • Departament Biologia Cel·lular, Fisiologia i ImmunologiaUniversitat Autònoma de Barcelona
Article

DOI: 10.1007/s10544-009-9393-6

Cite this article as:
Fernández-Rosas, E., Gómez, R., Ibañez, E. et al. Biomed Microdevices (2010) 12: 371. doi:10.1007/s10544-009-9393-6

Abstract

Microchips can be fabricated, using semiconductor technologies, at microscopic level to be introduced into living cells for monitoring of intracellular parameters at a single cell level. As a first step towards intracellular chips development, silicon and polysilicon microparticles of controlled shape and dimensions were fabricated and introduced into human macrophages and mouse embryos by phagocytosis and microinjection, respectively. Microparticles showed to be non-cytotoxic for macrophages and were found to be localized mainly inside early endosomes, in tight association with endosomal membrane, and more rarely in acidic compartments. Embryos with microinjected microparticles developed normally to the blastocyst stage, confirming the non-cytotoxic effect of the particles. In view of these results silicon and polysilicon microparticles can serve as the frame for future intracellular chips development and this technology opens the possibility of real complex devices to be used as sensors or actuators inside living cells.

Keywords

SiliconPolysiliconMicroparticleMEMSCytotoxicity

Abbreviations

CLSM

confocal laser scanning microscopy

FIB

focus ion beam

ICCs

IntraCellular Chips

MEMS

MicroElectroMechanical Systems

MP

microparticle

NP

nanoparticle

PF-MP

polystyrene fluorescent microspheres

pSi-MP

polysilicon MP

SEM

scanning electron microscope

Si-MP

Silicon MP

TEM

transmission electron microscopy

Supplementary material

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Copyright information

© Springer Science+Business Media, LLC 2009