Biomedical Microdevices

, Volume 12, Issue 1, pp 145–151

Patterning alginate hydrogels using light-directed release of caged calcium in a microfluidic device

  • Bor-han Chueh
  • Ying Zheng
  • Yu-suke Torisawa
  • Amy Y. Hsiao
  • Chunxi Ge
  • Susan Hsiong
  • Nathaniel Huebsch
  • Renny Franceschi
  • David J. Mooney
  • Shuichi Takayama
Article

DOI: 10.1007/s10544-009-9369-6

Cite this article as:
Chueh, B., Zheng, Y., Torisawa, Y. et al. Biomed Microdevices (2010) 12: 145. doi:10.1007/s10544-009-9369-6

Abstract

This paper describes a simple reversible hydrogel patterning method for 3D cell culture. Alginate gel is formed in select regions of a microfluidic device through light-triggered release of caged calcium. In the pre-gelled alginate solution, calcium is chelated by DM-nitrophen (DM-n) to prevent cross-linking of alginate. After sufficient UV exposure the caged calcium is released from DM-n causing alginate to cross-link. The effect of using different concentrations of calcium and chelating agents as well as the duration of UV exposure is described. Since the cross-linking is based on calcium concentration, the cross-linked alginate can easily be dissolved by EDTA. We also demonstrate application of this capability to patterned microscale 3D co-culture using endothelial cells and osteoblastic cells in a microchannel.

Keywords

MicrofluidicsAlginateHydrogelEndothelial cellsOsteoblastic cellsCaged calcium

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Bor-han Chueh
    • 1
    • 6
  • Ying Zheng
    • 1
  • Yu-suke Torisawa
    • 1
  • Amy Y. Hsiao
    • 1
  • Chunxi Ge
    • 2
  • Susan Hsiong
    • 3
  • Nathaniel Huebsch
    • 3
    • 4
  • Renny Franceschi
    • 2
  • David J. Mooney
    • 3
  • Shuichi Takayama
    • 1
    • 5
  1. 1.Biomedical EngineeringUniversity of MichiganAnn ArborUSA
  2. 2.Periodontics and Oral MedicineUniversity of MichiganAnn ArborUSA
  3. 3.School of Engineering and Applied ScienceHarvard UniversityCambridgeUSA
  4. 4.Harvard-MIT Division of Health Sciences and TechnologyCambridgeUSA
  5. 5.Macromolecular Science & Engineering programUniversity of MichiganAnn ArborUSA
  6. 6.Department of ChemistryStanford UniversityStanfordUSA