Biomedical Microdevices

, Volume 12, Issue 1, pp 145–151

Patterning alginate hydrogels using light-directed release of caged calcium in a microfluidic device

Authors

  • Bor-han Chueh
    • Biomedical EngineeringUniversity of Michigan
    • Department of ChemistryStanford University
  • Ying Zheng
    • Biomedical EngineeringUniversity of Michigan
  • Yu-suke Torisawa
    • Biomedical EngineeringUniversity of Michigan
  • Amy Y. Hsiao
    • Biomedical EngineeringUniversity of Michigan
  • Chunxi Ge
    • Periodontics and Oral MedicineUniversity of Michigan
  • Susan Hsiong
    • School of Engineering and Applied ScienceHarvard University
  • Nathaniel Huebsch
    • School of Engineering and Applied ScienceHarvard University
    • Harvard-MIT Division of Health Sciences and Technology
  • Renny Franceschi
    • Periodontics and Oral MedicineUniversity of Michigan
  • David J. Mooney
    • School of Engineering and Applied ScienceHarvard University
    • Biomedical EngineeringUniversity of Michigan
    • Macromolecular Science & Engineering programUniversity of Michigan
Article

DOI: 10.1007/s10544-009-9369-6

Cite this article as:
Chueh, B., Zheng, Y., Torisawa, Y. et al. Biomed Microdevices (2010) 12: 145. doi:10.1007/s10544-009-9369-6

Abstract

This paper describes a simple reversible hydrogel patterning method for 3D cell culture. Alginate gel is formed in select regions of a microfluidic device through light-triggered release of caged calcium. In the pre-gelled alginate solution, calcium is chelated by DM-nitrophen (DM-n) to prevent cross-linking of alginate. After sufficient UV exposure the caged calcium is released from DM-n causing alginate to cross-link. The effect of using different concentrations of calcium and chelating agents as well as the duration of UV exposure is described. Since the cross-linking is based on calcium concentration, the cross-linked alginate can easily be dissolved by EDTA. We also demonstrate application of this capability to patterned microscale 3D co-culture using endothelial cells and osteoblastic cells in a microchannel.

Keywords

MicrofluidicsAlginateHydrogelEndothelial cellsOsteoblastic cellsCaged calcium

Copyright information

© Springer Science+Business Media, LLC 2009