Article

Biomedical Microdevices

, Volume 12, Issue 1, pp 145-151

Patterning alginate hydrogels using light-directed release of caged calcium in a microfluidic device

  • Bor-han ChuehAffiliated withBiomedical Engineering, University of MichiganDepartment of Chemistry, Stanford University
  • , Ying ZhengAffiliated withBiomedical Engineering, University of Michigan
  • , Yu-suke TorisawaAffiliated withBiomedical Engineering, University of Michigan
  • , Amy Y. HsiaoAffiliated withBiomedical Engineering, University of Michigan
  • , Chunxi GeAffiliated withPeriodontics and Oral Medicine, University of Michigan
  • , Susan HsiongAffiliated withSchool of Engineering and Applied Science, Harvard University
  • , Nathaniel HuebschAffiliated withSchool of Engineering and Applied Science, Harvard UniversityHarvard-MIT Division of Health Sciences and Technology
  • , Renny FranceschiAffiliated withPeriodontics and Oral Medicine, University of Michigan
  • , David J. MooneyAffiliated withSchool of Engineering and Applied Science, Harvard University
    • , Shuichi TakayamaAffiliated withBiomedical Engineering, University of MichiganMacromolecular Science & Engineering program, University of Michigan Email author 

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Abstract

This paper describes a simple reversible hydrogel patterning method for 3D cell culture. Alginate gel is formed in select regions of a microfluidic device through light-triggered release of caged calcium. In the pre-gelled alginate solution, calcium is chelated by DM-nitrophen (DM-n) to prevent cross-linking of alginate. After sufficient UV exposure the caged calcium is released from DM-n causing alginate to cross-link. The effect of using different concentrations of calcium and chelating agents as well as the duration of UV exposure is described. Since the cross-linking is based on calcium concentration, the cross-linked alginate can easily be dissolved by EDTA. We also demonstrate application of this capability to patterned microscale 3D co-culture using endothelial cells and osteoblastic cells in a microchannel.

Keywords

Microfluidics Alginate Hydrogel Endothelial cells Osteoblastic cells Caged calcium