Article

Biomedical Microdevices

, Volume 12, Issue 1, pp 107-114

Water-assisted CO2 laser ablated glass and modified thermal bonding for capillary-driven bio-fluidic application

  • C. K. ChungAffiliated withDepartment of Mechanical Engineering, Center for Micro/Nano Science and Technology, National Cheng Kung UniversityInstitute of Nanotechnology and Microsystems Engineering, National Cheng Kung University Email author 
  • , H. C. ChangAffiliated withInstitute of Bio-medical Engineering, National Cheng Kung University
  • , T. R. ShihAffiliated withDepartment of Mechanical Engineering, Center for Micro/Nano Science and Technology, National Cheng Kung University
  • , S. L. LinAffiliated withDepartment of Mechanical Engineering, Center for Micro/Nano Science and Technology, National Cheng Kung University
  • , E. J. HsiaoAffiliated withDepartment of Mechanical Engineering, Center for Micro/Nano Science and Technology, National Cheng Kung University
  • , Y. S. ChenAffiliated withDepartment of Mechanical Engineering, Center for Micro/Nano Science and Technology, National Cheng Kung University
  • , E. C. ChangAffiliated withInstitute of Nanotechnology and Microsystems Engineering, National Cheng Kung University
  • , C. C. ChenAffiliated withInstitute of Bio-medical Engineering, National Cheng Kung University
  • , C. C. LinAffiliated withInstitute of Bio-medical Engineering, National Cheng Kung University

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Abstract

The glass-based microfluidic chip has widely been applied to the lab-on-a-chip for clotting tests. Here, we have demonstrated a capillary driven flow chip using the water-assisted CO2 laser ablation for crackless fluidic channels and holes as well as the modified low-temperature glass bonding with assistance of adhesive polymer film at 300°C. Effect of water depth on the laser ablation of glass quality was investigated. The surface hydrophilic property of glass and polymer film was measured by static contact angle method for hydrophilicity examination in comparison with the conventional polydimethylsiloxane (PDMS) material. Both low-viscosity deionized water and high-viscosity whole blood were used for testing the capillary-driving flow behavior. The preliminary coagulation testing in the Y-channel chip was also performed using whole blood and CaCl2 solution. The water-assisted CO2 laser processing can cool down glass during ablation for less temperature gradient to eliminate the crack. The modified glass bonding can simplify the conventional complex fabrication procedure of glass chips, such as high-temperature bonding, long consuming time and high cost. Moreover, the developed fluidic glass chip has the merit of hydrophilic behavior conquering the problem of traditional hydrophobic recovery of polymer fluidic chips and shows the ability to drive high-viscosity bio-fluids.

Keywords

Glass chip Microfluidic CO2 laser Hydrophilic Blood