Biomedical Microdevices

, Volume 12, Issue 1, pp 63–70

Nanostructured conducting polymer based reagentless capacitive immunosensor

Authors

  • Amay Jairaj Bandodkar
    • Department of Applied Chemistry, Institute of TechnologyBanaras Hindu University
  • Chetna Dhand
    • Department of Science and Technology Centre on Biomolecular ElectronicsNational Physical Laboratory
  • Sunil K. Arya
    • Department of Science and Technology Centre on Biomolecular ElectronicsNational Physical Laboratory
  • M. K. Pandey
    • Department of Science and Technology Centre on Biomolecular ElectronicsNational Physical Laboratory
    • Department of Science and Technology Centre on Biomolecular ElectronicsNational Physical Laboratory
    • Centre for NanoBioEngineering and SpintronicsChungnam National University
Article

DOI: 10.1007/s10544-009-9360-2

Cite this article as:
Bandodkar, A.J., Dhand, C., Arya, S.K. et al. Biomed Microdevices (2010) 12: 63. doi:10.1007/s10544-009-9360-2

Abstract

Nanostructured polyaniline (PANI) film electrophoretically fabricated onto indium-tin-oxide (ITO) coated glass plate has been utilized for development of an immunosensor based on capacitance change of a parallel plate capacitor (PPC) by covalently immobilizing anti-human IgG (Anti-HIgG) using N-ethyl-N’-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide chemistry. These fabricated PANI/ITO and Anti-HIgG/PANI/ITO plates have been characterized using scanning electron microscopy, cyclic voltammetry, differential pulse voltammetry and Fourier transform infra-red studies. The capacitance measurements indicate that dielectric medium of this biologically modified PPC (Anti-HIgG/PANI/ITO) is sensitive to HIgG in 5 − 5 × 105 ng mL−1 range and has lower detection limit of 1.87 ng mL−1. The observed results reveal that this Anti-HIgG modified PPC can be used as a robust, easy-to-use, reagentless, sensitive and selective immunosensor for estimation of human IgG.

Keywords

Polyaniline Electrophoretic deposition Parallel plate capacitor Immunosensor

Copyright information

© Springer Science+Business Media, LLC 2009