Abstract
In this paper, we propose a method to construct three-dimensional curved microstructures with easy control of the size, position and shape, by exploiting the elasticity of poly(dimethylsiloxane) (PDMS) membranes and basic physics. For this end, we developed the method to handle thin PDMS membrane safely, and to replicate PDMS microstructure from the PDMS mold. Using this method, we demonstrated two potential applications: (1) the use of concave well for the formation of embryoid body (EB) to differentiate into neuronal cells, and (2) the fabrication of SU-8 and hydrogel microparticles having diverse curved shapes. The curved structures were successfully fabricated with simple process, and EBs were formed in the concave well and differentiated into the neuronal cells. Microparticles with diverse shapes were fabricated from a range of materials for potential use as drug carrier and pH responsive micro-actuator elements.
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Acknowledgements
This study was supported by a grant of the NRL (National Research Lab) program, the Korea Science and Engineering Foundation (KOSEF), Republic of Korea (R0A-2007-000-20086-0), and by a grant of the Korea Healthcare technology R&D Project, Ministry for Health, Welfare and Family Affairs, Republic of Korea (A040032). Dr. J. Y. Park was supported by the Korea Research Foundation Grant funded by the Korean Government (KRF-2008-357-D00030).
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Dae Ho Lee and Joong Yull Park contributed equally to this work.
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Lee, D.H., Park, J.Y., Lee, EJ. et al. Fabrication of three-dimensional microarray structures by controlling the thickness and elasticity of poly(dimethylsiloxane) membrane. Biomed Microdevices 12, 49–54 (2010). https://doi.org/10.1007/s10544-009-9357-x
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DOI: https://doi.org/10.1007/s10544-009-9357-x