Biomedical Microdevices

, Volume 12, Issue 1, pp 49–54

Fabrication of three-dimensional microarray structures by controlling the thickness and elasticity of poly(dimethylsiloxane) membrane

  • Dae Ho Lee
  • Joong Yull Park
  • Eun-Joong Lee
  • Yoon Young Choi
  • Gu Han Kwon
  • Beop-Min Kim
  • Sang-Hoon Lee
Article

DOI: 10.1007/s10544-009-9357-x

Cite this article as:
Lee, D.H., Park, J.Y., Lee, E. et al. Biomed Microdevices (2010) 12: 49. doi:10.1007/s10544-009-9357-x

Abstract

In this paper, we propose a method to construct three-dimensional curved microstructures with easy control of the size, position and shape, by exploiting the elasticity of poly(dimethylsiloxane) (PDMS) membranes and basic physics. For this end, we developed the method to handle thin PDMS membrane safely, and to replicate PDMS microstructure from the PDMS mold. Using this method, we demonstrated two potential applications: (1) the use of concave well for the formation of embryoid body (EB) to differentiate into neuronal cells, and (2) the fabrication of SU-8 and hydrogel microparticles having diverse curved shapes. The curved structures were successfully fabricated with simple process, and EBs were formed in the concave well and differentiated into the neuronal cells. Microparticles with diverse shapes were fabricated from a range of materials for potential use as drug carrier and pH responsive micro-actuator elements.

Keywords

PDMS membraneCurved microstructureElasticity of PDMSEmbryoid body

Supplementary material

10544_2009_9357_MOESM1_ESM.pdf (673 kb)
ESM1(PDF 672 kb)

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Dae Ho Lee
    • 1
  • Joong Yull Park
    • 1
    • 2
  • Eun-Joong Lee
    • 1
  • Yoon Young Choi
    • 1
  • Gu Han Kwon
    • 1
  • Beop-Min Kim
    • 1
  • Sang-Hoon Lee
    • 1
  1. 1.Department of Biomedical Engineering, College of Health ScienceKorea UniversitySeoulRepublic of Korea
  2. 2.Department of Biomedical Engineering, College of EngineeringUniversity of MichiganAnn ArborUSA