Article

Biomedical Microdevices

, Volume 12, Issue 1, pp 35-40

Electroporation based on hydrodynamic focusing of microfluidics with low dc voltage

  • Tao ZhuAffiliated withDepartment of Biomedical Engineering, College of Engineering, Peking UniversityAcademy of Advanced Interdisciplinary Studies, Peking University
  • , Chunxiong LuoAffiliated withCenter for Microfluidics and Nanotechnology, School of Physics, Peking UniversityAcademy of Advanced Interdisciplinary Studies, Peking University
  • , Jianyong HuangAffiliated withDepartment of Biomedical Engineering, College of Engineering, Peking UniversityAcademy of Advanced Interdisciplinary Studies, Peking University
  • , Chunyang XiongAffiliated withDepartment of Biomedical Engineering, College of Engineering, Peking UniversityAcademy of Advanced Interdisciplinary Studies, Peking University Email author 
  • , Qi OuyangAffiliated withCenter for Microfluidics and Nanotechnology, School of Physics, Peking UniversityAcademy of Advanced Interdisciplinary Studies, Peking University
  • , Jing FangAffiliated withDepartment of Biomedical Engineering, College of Engineering, Peking UniversityAcademy of Advanced Interdisciplinary Studies, Peking University

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Abstract

Microfluidics-based cell electroporation has many advantages in delivering small molecules into cells. In this study, hydrodynamic focusing of fluids with different conductivities has been used for high through-put cell electroporation at low voltage (<3 V) of continuous direct current (dc) power. Simulation results showed that an input voltage of only 1.5 V could generate an electric field intensity of about 1.17 kV cm−1 across the cell suspension flow in the squeezed area. The electropermeation of yeast cell was observed, showing a permeabilization percentage up to 70%.

Keywords

Cell electroporation Microfluidics Hydrodynamic focusing