Biomedical Microdevices

, Volume 12, Issue 1, pp 35–40

Electroporation based on hydrodynamic focusing of microfluidics with low dc voltage

  • Tao Zhu
  • Chunxiong Luo
  • Jianyong Huang
  • Chunyang Xiong
  • Qi Ouyang
  • Jing Fang
Article

DOI: 10.1007/s10544-009-9355-z

Cite this article as:
Zhu, T., Luo, C., Huang, J. et al. Biomed Microdevices (2010) 12: 35. doi:10.1007/s10544-009-9355-z
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Abstract

Microfluidics-based cell electroporation has many advantages in delivering small molecules into cells. In this study, hydrodynamic focusing of fluids with different conductivities has been used for high through-put cell electroporation at low voltage (<3 V) of continuous direct current (dc) power. Simulation results showed that an input voltage of only 1.5 V could generate an electric field intensity of about 1.17 kV cm−1 across the cell suspension flow in the squeezed area. The electropermeation of yeast cell was observed, showing a permeabilization percentage up to 70%.

Keywords

Cell electroporationMicrofluidicsHydrodynamic focusing

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Tao Zhu
    • 1
    • 3
  • Chunxiong Luo
    • 2
    • 3
  • Jianyong Huang
    • 1
    • 3
  • Chunyang Xiong
    • 1
    • 3
  • Qi Ouyang
    • 2
    • 3
  • Jing Fang
    • 1
    • 3
  1. 1.Department of Biomedical Engineering, College of EngineeringPeking UniversityBeijingChina
  2. 2.Center for Microfluidics and Nanotechnology, School of PhysicsPeking UniversityBeijingChina
  3. 3.Academy of Advanced Interdisciplinary StudiesPeking UniversityBeijingChina