Biomedical Microdevices

, Volume 12, Issue 1, pp 13–21

Targeted cell adhesion on selectively micropatterned polymer arrays on a poly(dimethylsiloxane) surface

Authors

  • Linzhi Tang
    • Gachon BioNano Research Institute & Division of BioNano Technology and College of BioNano TechnologyKyungwon University
  • Junhong Min
    • Gachon BioNano Research Institute & Division of BioNano Technology and College of BioNano TechnologyKyungwon University
  • Eun-Cheol Lee
    • Gachon BioNano Research Institute & Division of BioNano Technology and College of BioNano TechnologyKyungwon University
  • Jong Sung Kim
    • Department of Chemical & BioengineeringKyungwon University
    • Gachon BioNano Research Institute & Division of BioNano Technology and College of BioNano TechnologyKyungwon University
Article

DOI: 10.1007/s10544-009-9353-1

Cite this article as:
Tang, L., Min, J., Lee, E. et al. Biomed Microdevices (2010) 12: 13. doi:10.1007/s10544-009-9353-1

Abstract

Herein, we introduce the fabrication of polymer micropattern arrays on a chemically inert poly(dimethylsiloxane) (PDMS) surface and employ them for the selective adhesion of cells. To fabricate the micropattern arrays, a mercapto-ester—based photocurable adhesive was coated onto a mercaptosilane—coated PDMS surface and photopolymerized using a photomask to obtain patterned arrays at the microscale level. Robust polymer patterns, 380 µm in diameter, were successfully fabricated onto a PDMS surface, and cells were selectively targeted toward the patterned regions. Next, the performance of the cell adhesion was observed by anchoring cell adhesive linker, an RGD oligopeptide, on the surface of the mercapto-ester—based adhesive-cured layer. The successful anchoring of the RGD linker was confirmed through various surface characterizations such as water contact angle measurement, XPS analysis, FT-IR analysis, and AFM measurement. The micropatterning of a photocurable adhesive onto a PDMS surface can provide high structural rigidity, a highly–adhesive surface, and a physical pathway for selective cell adhesion, while the incorporated polymer micropattern arrays inside a PDMS microfluidic device can serve as a microfluidic platform for disease diagnoses and high-throughput drug screening.

Keywords

Polymer micropattern array Mercapto-ester adhesive Mercaptosilane Photopolymerization Cell adhesion RGD linker

Copyright information

© Springer Science+Business Media, LLC 2009