Targeted cell adhesion on selectively micropatterned polymer arrays on a poly(dimethylsiloxane) surface Authors
First Online: 15 September 2009 DOI:
Cite this article as: Tang, L., Min, J., Lee, E. et al. Biomed Microdevices (2010) 12: 13. doi:10.1007/s10544-009-9353-1
Herein, we introduce the fabrication of polymer micropattern arrays on a chemically inert poly(dimethylsiloxane) (PDMS) surface and employ them for the selective adhesion of cells. To fabricate the micropattern arrays, a mercapto-ester—based photocurable adhesive was coated onto a mercaptosilane—coated PDMS surface and photopolymerized using a photomask to obtain patterned arrays at the microscale level. Robust polymer patterns, 380 µm in diameter, were successfully fabricated onto a PDMS surface, and cells were selectively targeted toward the patterned regions. Next, the performance of the cell adhesion was observed by anchoring cell adhesive linker, an RGD oligopeptide, on the surface of the mercapto-ester—based adhesive-cured layer. The successful anchoring of the RGD linker was confirmed through various surface characterizations such as water contact angle measurement, XPS analysis, FT-IR analysis, and AFM measurement. The micropatterning of a photocurable adhesive onto a PDMS surface can provide high structural rigidity, a highly–adhesive surface, and a physical pathway for selective cell adhesion, while the incorporated polymer micropattern arrays inside a PDMS microfluidic device can serve as a microfluidic platform for disease diagnoses and high-throughput drug screening.
Polymer micropattern array
J.A. Barron, P. Wu, H.D. Ladouceur, B.R. Ringeisen, Biological laser printing: A novel technique for creating heterogeneous 3-dimensional cell patterns. Biomed. Microdevices
, 139–147 (2004)
J.A. Burdick, A. Khademhosseini, R. Langer, Fabrication of gradient hydrogels using a microfluidics/photopolymerization process. Langmuir
, 5153–5156 (2004)
V.J. Cadarso, A. Llobera, G. Villanueva, V. Seidemann, S. Büttgenbach, J.A. Plaza, Polymer microoptoelectromechanical systems: Accelerometers and variable optical attenuators, Sens. Actuator, A
, 147–153 (2008)
S.C. Calaghan, E. White, S. Bedut, J.-Y. Le Guennec, Cytochalasin D reduces Ca
sensitivity and maximum tension via interactions with myofilaments in skinned rat cardiac myocytes. J. Physiol.
(2), 405–411 (2000)
E. Cimetta, S. Pizzato, S. Bollini, E. Serena, P. De Coppi, N. Elvassore, Production of arrays of cardiac and skeletal muscle myofibers by micropatterning techniques on a soft substrate. Biomed. Microdevices
, 389–400 (2009)
J. Fukuda, Y. Sakai, K. Nakazawa, Novel hepatocyte culture system developed using microfabrication and collagen/polyethylene glycol microcontact printing. Biomaterials
, 1061–1070 (2006)
M. Goto, T. Tsukahara, K. Sato, T. Kitamori, Micro- and nanometer-scale patterned surface in a microchannel for cell culture in microfluidic devices. Anal. Bioanal. Chem.
, 817–823 (2008)
D.S. Gray, J. Tien, C.S. Chen, Repositioning of cells by mechanotaxis on surfaces with micropatterned Young’s modulus. J. Biomed. Mater. Res.
, 605–614 (2003)
Y. Ito, Surface micropatterning to regulate cell functions. Biomaterials
, 2333–2342 (1999)
K. Itoga, J. Kobayashi, M. Yamato, A. Kikuchi, T. Okano, Maskless liquid-crystal-display projection photolithography for improved design flexibility of cellular micropatterns. Biomaterials
, 3005–3009 (2006)
X. Jiang, S. Takayama, X. Qian, E. Ostuni, H. Wu, N. Bowden, P. LeDuc, D.E. Ingber, G.M. Whitesides, Controlling mammalian cell spreading and cytoskeletal arrangement with conveniently fabricated continuous wavy features on poly(dimethylsiloxane). Langmuir
, 3273–3280 (2002)
J.N. Lee, X. Jiang, D. Ryan, G.M. Whitesides, Compatibility of mammalian cells on surfaces of poly(dimethylsiloxane). Langmuir
, 11684–11691 (2004)
N.Y. Lee, J.R. Lim, Y.S. Kim, Selective patterning and immobilization of biomolecules within precisely-defined micro-reservoirs. Biosens. Bioelectron.
, 2188–2193 (2006a)
N.Y. Lee, J.R. Lim, M.J. Lee, S. Park, Y.S. Kim, Multilayer transfer printing on microreservoir-patterned substrate employing hydrophilic composite mold for selective immobilization of biomolecules. Langmuir
, 7689–7694 (2006b)
D. Lehnert, B. Wehrle-Haller, C. David, U. Weiland, C. Ballestrem, B.A. Imhof, M. Bastmeyer, Cell behaviour on micropatterned substrata: limits of extracellular matrix geometry for spreading and adhesion. J. Cell. Sci.
, 41–52 (2004)
C.-M. Lo, H.-B. Wang, M. Dembo, Y.-L. Wang, Cell movement is guided by the rigidity of the substrate. Biophys J.
, 144–152 (2000)
R. Lovchik, C. von Arx, A. Viviani, E. Delamarche, Cellular microarrays for use with capillary-driven microfluidics. Anal. Bioanal. Chem.
, 801–808 (2008)
A. Mata, C. Boehm, A.J. Fleischman, G. Muschler, S. Roy, Growth of connective tissue progenitor cells on microtextured polydimethylsiloxane surfaces. J. Biomed. Mater. Res.
, 499–506 (2002)
M. Ochsner, M.R. Dusseiller, H.M. Grandin, S. Luna-Morris, M. Textor, V. Vogel, M.L. Smith, Micro-well arrays for 3D shape control and high resolution analysis of single cells. Lab Chip
, 1074–1077 (2007)
B. Pinto-Iguanero, A. Olivares-Pérez, I. Fuentes-Tapia, Holographic material film composed by Norland Noa 65 adhesive. Opt. Mater.
, 225–232 (2002)
L. Richert, F. Boulmedais, P. Lavalle, J. Mutterer, E. Ferreux, G. Decher, P. Schaaf, J.-C. Voegel, C. Picart, Improvement of stability and cell adhesion properties of polyelectrolyte multilayer films by chemical cross-linking. Biomacromolecules
, 284–294 (2004)
S. Svedhem, D. Dahlborg, J. Ekeroth, J. Kelly, F. Höök, J. Gold, In situ peptide-modified supported lipid bilayers for controlled cell attachment. Langmuir
, 6730–6736 (2003)
T. Tzvetkova-Chevolleau, A. Stéphanou, D. Fuard, J. Ohayon, P. Schiavone, P. Tracqui, The motility of normal and cancer cells in response to the combined influence of the substrate rigidity and anisotropic microstructure. Biomaterials
, 1541–1551 (2008)
S.Y.U. Venyaminov, N.N. Kalnin, Quantitative IR spectrophotometry of peptide compounds in water (H
O) solutions. I. Spectral parameters of amino acid residue absorption band. Biopolymers
, 1243–1257 (1990)
K. Yamauchi, H. Hojo, Y. Yamamoto, T. Tanabe, Enhanced cell adhesion on RGDS-carrying keratin film. Mat. Sci. Eng. C
23, 467–472 (2003) Copyright information
© Springer Science+Business Media, LLC 2009