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3D microfabricated bioreactor with capillaries

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Abstract

We present in this paper the implementation of an innovative three dimensional (3D) microfabrication technology coupled with numerical simulation to enhance the mass transport in 3D cell culture. The core of this microfabrication technology is a high-resolution projection micro stereolithography (PμSL) using a spatial light modulator as a dynamic mask which enables a parallel fabrication of highly complex 3D microstructures. In this work, a set of poly (ethylene glycol) microfabricated bioreactors are demonstrated with PμSL technology. We observed both experimentally and numerically the regulation of metabolism and the growth of yeast cells by controlling the density of micro-capillaries. Further development of these 3D microfabricated bioreactors is expected to provide artificially constructed tissues for clinical applications.

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Acknowledgement

This project was supported by the Gauthier Exploratory Research Foundation.

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Authors and Affiliations

  1. Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, 158 Mechanical Engineering Building, MC-244, 1206 West Green Street, Urbana, IL, 61801-2906, USA

    Chunguang Xia & Nicholas X. Fang

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  1. Chunguang Xia
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  2. Nicholas X. Fang
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Correspondence to Nicholas X. Fang.

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Xia, C., Fang, N.X. 3D microfabricated bioreactor with capillaries. Biomed Microdevices 11, 1309–1315 (2009). https://doi.org/10.1007/s10544-009-9350-4

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