Biomedical Microdevices

, 11:1289

Disposable plastic microreactors for genomic analyses

  • S. Sabella
  • G. Vecchio
  • P. P. Pompa
  • G. Maruccio
  • L. Sanarica
  • A. Della Torre
  • G. De Bellis
  • G. Caramenti
  • C. Consolandi
  • M. Severgnini
  • R. Cingolani
  • R. Rinaldi
Article

DOI: 10.1007/s10544-009-9348-y

Cite this article as:
Sabella, S., Vecchio, G., Pompa, P.P. et al. Biomed Microdevices (2009) 11: 1289. doi:10.1007/s10544-009-9348-y
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Abstract

We show the design, development and assessment of disposable, biocompatible, fully plastic microreactors, which are demonstrated to be highly efficient for genomic analyses, such as amplification of DNA, quantitative analyses in real time, multiplex PCR (both in terms of efficiency and selectivity), as compared to conventional laboratory equipment for PCR. The plastic microreactors can easily be coupled to reusable hardware, enabling heating/cooling processes and, in the case of qPCR applications, the real-time detection of the signal from a suitable fluorescent reporter present in the reaction mixture during the analysis. The low cost production of these polymeric microreactors, along with their applicability to a wide range of biochemical targets, may open new perspectives towards practical applications of biochips for point of care diagnostics.

Keywords

Plastic microreactorsGenomic analysesReal time PCRBiochip

Abbreviations

PCR

Polymerase Chain Reaction

qPCR

quantitative PCR

PDMS

Polydimethylsiloxane

PMMA

Polymethyl-methacrylate

BSA

Bovine serum albumin

PEG

Polyethylene-glycol

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • S. Sabella
    • 1
    • 3
  • G. Vecchio
    • 1
  • P. P. Pompa
    • 1
  • G. Maruccio
    • 1
  • L. Sanarica
    • 1
  • A. Della Torre
    • 1
  • G. De Bellis
    • 2
  • G. Caramenti
    • 2
  • C. Consolandi
    • 2
  • M. Severgnini
    • 2
  • R. Cingolani
    • 1
  • R. Rinaldi
    • 1
  1. 1.National Nanotechnology Laboratory of CNR-INFMIIT Research UnitLecceItaly
  2. 2.Institute of Biomedical TechnologiesNational Research Council (ITB-CNR)SegrateItaly
  3. 3.National Nanotechnology Laboratory of INFM-CNRItalian Institute of Technology (IIT)LecceItaly