Article

Biomedical Microdevices

, Volume 11, Issue 6, pp 1289-1295

Disposable plastic microreactors for genomic analyses

  • S. SabellaAffiliated withNational Nanotechnology Laboratory of CNR-INFM, IIT Research UnitNational Nanotechnology Laboratory of INFM-CNR, Italian Institute of Technology (IIT) Email author 
  • , G. VecchioAffiliated withNational Nanotechnology Laboratory of CNR-INFM, IIT Research Unit
  • , P. P. PompaAffiliated withNational Nanotechnology Laboratory of CNR-INFM, IIT Research Unit
  • , G. MaruccioAffiliated withNational Nanotechnology Laboratory of CNR-INFM, IIT Research Unit
  • , L. SanaricaAffiliated withNational Nanotechnology Laboratory of CNR-INFM, IIT Research Unit
  • , A. Della TorreAffiliated withNational Nanotechnology Laboratory of CNR-INFM, IIT Research Unit
  • , G. De BellisAffiliated withInstitute of Biomedical Technologies, National Research Council (ITB-CNR)
  • , G. CaramentiAffiliated withInstitute of Biomedical Technologies, National Research Council (ITB-CNR)
  • , C. ConsolandiAffiliated withInstitute of Biomedical Technologies, National Research Council (ITB-CNR)
    • , M. SevergniniAffiliated withInstitute of Biomedical Technologies, National Research Council (ITB-CNR)
    • , R. CingolaniAffiliated withNational Nanotechnology Laboratory of CNR-INFM, IIT Research Unit
    • , R. RinaldiAffiliated withNational Nanotechnology Laboratory of CNR-INFM, IIT Research Unit

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Abstract

We show the design, development and assessment of disposable, biocompatible, fully plastic microreactors, which are demonstrated to be highly efficient for genomic analyses, such as amplification of DNA, quantitative analyses in real time, multiplex PCR (both in terms of efficiency and selectivity), as compared to conventional laboratory equipment for PCR. The plastic microreactors can easily be coupled to reusable hardware, enabling heating/cooling processes and, in the case of qPCR applications, the real-time detection of the signal from a suitable fluorescent reporter present in the reaction mixture during the analysis. The low cost production of these polymeric microreactors, along with their applicability to a wide range of biochemical targets, may open new perspectives towards practical applications of biochips for point of care diagnostics.

Keywords

Plastic microreactors Genomic analyses Real time PCR Biochip