Biomedical Microdevices

, 11:1279

A self-contained fully-enclosed microfluidic cartridge for lab on a chip

Authors

    • Institute of Microelectronics, 11 Science Park Road
  • Lih Feng Cheow
    • Institute of Microelectronics, 11 Science Park Road
  • Kum-Cheong Tang
    • Institute of Microelectronics, 11 Science Park Road
  • Shien-Eit Yong
    • Institute of Microelectronics, 11 Science Park Road
  • Eleana Kye-Zheng Ong
    • Institute of Microelectronics, 11 Science Park Road
  • Lionel Wong
    • Institute of Microelectronics, 11 Science Park Road
  • William Cheng-Yong Teo
    • Institute of Microelectronics, 11 Science Park Road
  • Hongmiao Ji
    • Institute of Microelectronics, 11 Science Park Road
  • Siti Rafeah
    • Institute of Microelectronics, 11 Science Park Road
  • Chen Yu
    • Institute of Microelectronics, 11 Science Park Road
Article

DOI: 10.1007/s10544-009-9347-z

Cite this article as:
Yobas, L., Feng Cheow, L., Tang, K. et al. Biomed Microdevices (2009) 11: 1279. doi:10.1007/s10544-009-9347-z

Abstract

We describe a self-contained fully-enclosed cartridge for lab-on-a-chip applications where sample and reagents can be applied sequentially as is performed in a heterogeneous immunoassay, or nucleic acid extraction. Both the self-contained and fully-enclosed features of the cartridge are sought to ensure its safe use in the field by unskilled staff. Simplicity in cartridge design and operation is obtained via adopting a valveless concept whereby reagents are stored and used in the form of liquid plugs isolated by air spacers around a fluidic loop. Functional components integrated in the loop include a microfluidic chip specific to the target application, a novel peristaltic pump to displace the liquid plugs, and a pair of removable tubing segments where one is used to introduce biological sample and while the other is to collect eluant. The novel pump is fabricated through soft-lithography technique and works by pinching a planar channel under stainless-steel ball bearings that have been magnetically loaded. The utility of the cartridge is demonstrated for automated extraction and purification of nucleic acids (DNA) from a cell lysate on a battery-operated portable system. The cartridge shown here can be further extended to sample-in-answer-out diagnostic tests.

Keywords

Microfluidics Lab on a chip Cartridge Nucleic acid Point of care

Copyright information

© Springer Science+Business Media, LLC 2009